Cargando…

MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows

MiR‐24‐3p, a broadly conserved, small, noncoding RNA, is abundantly expressed in mammary tissue. However, its regulatory role in this tissue remains poorly understood. It was predicted that miR‐24‐3p targets the 3′ untranslated region (3′‐UTR) of multiple endocrine neoplasia type 1 (MEN1), an import...

Descripción completa

Detalles Bibliográficos
Autores principales: Qiaoqiao, Cao, Li, Honghui, Liu, Xue, Yan, Zhengui, Zhao, Meng, Xu, Zhongjin, Wang, Zhonghua, Shi, Kerong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282567/
https://www.ncbi.nlm.nih.gov/pubmed/30221364
http://dx.doi.org/10.1002/jcp.27017
_version_ 1783379022134116352
author Qiaoqiao, Cao
Li, Honghui
Liu, Xue
Yan, Zhengui
Zhao, Meng
Xu, Zhongjin
Wang, Zhonghua
Shi, Kerong
author_facet Qiaoqiao, Cao
Li, Honghui
Liu, Xue
Yan, Zhengui
Zhao, Meng
Xu, Zhongjin
Wang, Zhonghua
Shi, Kerong
author_sort Qiaoqiao, Cao
collection PubMed
description MiR‐24‐3p, a broadly conserved, small, noncoding RNA, is abundantly expressed in mammary tissue. However, its regulatory role in this tissue remains poorly understood. It was predicted that miR‐24‐3p targets the 3′ untranslated region (3′‐UTR) of multiple endocrine neoplasia type 1 (MEN1), an important regulatory factor in mammary tissue. The objective of this study was to investigate the function of miR‐24‐3p in mammary cells. Using a luciferase assay in mammary epithelial cells (MAC‐T), miR‐24‐3p was confirmed to target the 3′‐UTR of MEN1. Furthermore, miR‐24‐3p negatively regulated the expression of the MEN1 gene and its encoded protein, menin. miR‐24‐3p enhanced proliferation of MAC‐T by promoting G1/S phase progression. MiR‐24‐3p also regulated the expression of key factors involved in phosphatidylinositol‐3‐kinase/protein kinase B/mammalian target of rapamycin and Janus kinase/signal transducer and activators of transcription signaling pathways, therefore controlling milk protein synthesis in epithelial cells. Thus, miR‐24‐3p appears to act on MAC‐T by targeting MEN1. The expression of miR‐24‐3p was controlled by MEN1/menin, indicating a negative feedback loop between miR‐24‐3p and MEN1/menin. The negatively inhibited expression pattern of miR‐24‐3p and MEN1 was active in mammary tissues at different lactation stages. The feedback mechanism is a new concept to further understand the lactation cycle of mammary glands and can possibly to be manipulated to improve milk yield and quality.
format Online
Article
Text
id pubmed-6282567
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-62825672018-12-11 MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows Qiaoqiao, Cao Li, Honghui Liu, Xue Yan, Zhengui Zhao, Meng Xu, Zhongjin Wang, Zhonghua Shi, Kerong J Cell Physiol Original Research Articles MiR‐24‐3p, a broadly conserved, small, noncoding RNA, is abundantly expressed in mammary tissue. However, its regulatory role in this tissue remains poorly understood. It was predicted that miR‐24‐3p targets the 3′ untranslated region (3′‐UTR) of multiple endocrine neoplasia type 1 (MEN1), an important regulatory factor in mammary tissue. The objective of this study was to investigate the function of miR‐24‐3p in mammary cells. Using a luciferase assay in mammary epithelial cells (MAC‐T), miR‐24‐3p was confirmed to target the 3′‐UTR of MEN1. Furthermore, miR‐24‐3p negatively regulated the expression of the MEN1 gene and its encoded protein, menin. miR‐24‐3p enhanced proliferation of MAC‐T by promoting G1/S phase progression. MiR‐24‐3p also regulated the expression of key factors involved in phosphatidylinositol‐3‐kinase/protein kinase B/mammalian target of rapamycin and Janus kinase/signal transducer and activators of transcription signaling pathways, therefore controlling milk protein synthesis in epithelial cells. Thus, miR‐24‐3p appears to act on MAC‐T by targeting MEN1. The expression of miR‐24‐3p was controlled by MEN1/menin, indicating a negative feedback loop between miR‐24‐3p and MEN1/menin. The negatively inhibited expression pattern of miR‐24‐3p and MEN1 was active in mammary tissues at different lactation stages. The feedback mechanism is a new concept to further understand the lactation cycle of mammary glands and can possibly to be manipulated to improve milk yield and quality. John Wiley and Sons Inc. 2018-09-17 2019-02 /pmc/articles/PMC6282567/ /pubmed/30221364 http://dx.doi.org/10.1002/jcp.27017 Text en © 2018 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Research Articles
Qiaoqiao, Cao
Li, Honghui
Liu, Xue
Yan, Zhengui
Zhao, Meng
Xu, Zhongjin
Wang, Zhonghua
Shi, Kerong
MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title_full MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title_fullStr MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title_full_unstemmed MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title_short MiR‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
title_sort mir‐24‐3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282567/
https://www.ncbi.nlm.nih.gov/pubmed/30221364
http://dx.doi.org/10.1002/jcp.27017
work_keys_str_mv AT qiaoqiaocao mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT lihonghui mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT liuxue mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT yanzhengui mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT zhaomeng mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT xuzhongjin mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT wangzhonghua mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows
AT shikerong mir243pregulatescellproliferationandmilkproteinsynthesisofmammaryepithelialcellsthroughmeninindairycows