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Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum

The eukaryotic ribonucleic acid (RNA) exosome is a versatile multiribonuclease complex that mediates the processing, surveillance, and degradation of virtually all classes of RNA in both the nucleus and cytoplasm. The complex, composed of 10 to 11 subunits, has been widely described in many organism...

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Autores principales: Jiang, Ning, Yu, Shengchao, Yang, Na, Feng, Ying, Sang, Xiaoyu, Wang, Yao, Wahlgren, Mats, Chen, Qijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282785/
https://www.ncbi.nlm.nih.gov/pubmed/29664138
http://dx.doi.org/10.1111/jeu.12625
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author Jiang, Ning
Yu, Shengchao
Yang, Na
Feng, Ying
Sang, Xiaoyu
Wang, Yao
Wahlgren, Mats
Chen, Qijun
author_facet Jiang, Ning
Yu, Shengchao
Yang, Na
Feng, Ying
Sang, Xiaoyu
Wang, Yao
Wahlgren, Mats
Chen, Qijun
author_sort Jiang, Ning
collection PubMed
description The eukaryotic ribonucleic acid (RNA) exosome is a versatile multiribonuclease complex that mediates the processing, surveillance, and degradation of virtually all classes of RNA in both the nucleus and cytoplasm. The complex, composed of 10 to 11 subunits, has been widely described in many organisms. Bioinformatic analyses revealed that there may be also an exosome‐like complex in Plasmodium falciparum, a parasite of great importance in public health, with eight predicted subunits having high sequence similarity to their counterparts in yeast and human. In this work, the putative RNA catalytic components, designated as PfRrp4, PfRrp41, PfDis3, and PfRrp6, were identified and systematically analyzed. Quantitative polymerase chain reaction (QPCR) analyses suggested that all of them were transcribed steadily throughout the asexual stage. The expression of these proteins was determined by Western blot, and their localization narrowed to the cytoplasm of the parasite by indirect immunofluorescence. The recombinant proteins of PfRrp41, PfDis3, and PfRrp6 exhibited catalytic activity for single‐stranded RNA (ssRNA), whereas PfRrp4 showed no processing activity of both ssRNA and dsRNA. The identification of these putative components of the RNA exosome complex opens up new perspectives for a deep understanding of RNA metabolism in the malarial parasite P. falciparum.
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spelling pubmed-62827852018-12-11 Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum Jiang, Ning Yu, Shengchao Yang, Na Feng, Ying Sang, Xiaoyu Wang, Yao Wahlgren, Mats Chen, Qijun J Eukaryot Microbiol Original Articles The eukaryotic ribonucleic acid (RNA) exosome is a versatile multiribonuclease complex that mediates the processing, surveillance, and degradation of virtually all classes of RNA in both the nucleus and cytoplasm. The complex, composed of 10 to 11 subunits, has been widely described in many organisms. Bioinformatic analyses revealed that there may be also an exosome‐like complex in Plasmodium falciparum, a parasite of great importance in public health, with eight predicted subunits having high sequence similarity to their counterparts in yeast and human. In this work, the putative RNA catalytic components, designated as PfRrp4, PfRrp41, PfDis3, and PfRrp6, were identified and systematically analyzed. Quantitative polymerase chain reaction (QPCR) analyses suggested that all of them were transcribed steadily throughout the asexual stage. The expression of these proteins was determined by Western blot, and their localization narrowed to the cytoplasm of the parasite by indirect immunofluorescence. The recombinant proteins of PfRrp41, PfDis3, and PfRrp6 exhibited catalytic activity for single‐stranded RNA (ssRNA), whereas PfRrp4 showed no processing activity of both ssRNA and dsRNA. The identification of these putative components of the RNA exosome complex opens up new perspectives for a deep understanding of RNA metabolism in the malarial parasite P. falciparum. John Wiley and Sons Inc. 2018-05-07 2018 /pmc/articles/PMC6282785/ /pubmed/29664138 http://dx.doi.org/10.1111/jeu.12625 Text en © 2018 The Authors Journal of Eukaryotic Microbiology published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Jiang, Ning
Yu, Shengchao
Yang, Na
Feng, Ying
Sang, Xiaoyu
Wang, Yao
Wahlgren, Mats
Chen, Qijun
Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title_full Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title_fullStr Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title_full_unstemmed Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title_short Characterization of the Catalytic Subunits of the RNA Exosome‐like Complex in Plasmodium falciparum
title_sort characterization of the catalytic subunits of the rna exosome‐like complex in plasmodium falciparum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282785/
https://www.ncbi.nlm.nih.gov/pubmed/29664138
http://dx.doi.org/10.1111/jeu.12625
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