A Photoenzymatic NADH Regeneration System

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD(+) into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The...

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Autores principales: Höfler, Georg T., Fernández‐Fueyo, Elena, Pesic, Milja, Younes, Sabry H., Choi, Eun‐Gyu, Kim, Yong H., Urlacher, Vlada B., Arends, Isabel W. C. E., Hollmann, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283237/
https://www.ncbi.nlm.nih.gov/pubmed/30192991
http://dx.doi.org/10.1002/cbic.201800530
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author Höfler, Georg T.
Fernández‐Fueyo, Elena
Pesic, Milja
Younes, Sabry H.
Choi, Eun‐Gyu
Kim, Yong H.
Urlacher, Vlada B.
Arends, Isabel W. C. E.
Hollmann, Frank
author_facet Höfler, Georg T.
Fernández‐Fueyo, Elena
Pesic, Milja
Younes, Sabry H.
Choi, Eun‐Gyu
Kim, Yong H.
Urlacher, Vlada B.
Arends, Isabel W. C. E.
Hollmann, Frank
author_sort Höfler, Georg T.
collection PubMed
description A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD(+) into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.
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spelling pubmed-62832372018-12-14 A Photoenzymatic NADH Regeneration System Höfler, Georg T. Fernández‐Fueyo, Elena Pesic, Milja Younes, Sabry H. Choi, Eun‐Gyu Kim, Yong H. Urlacher, Vlada B. Arends, Isabel W. C. E. Hollmann, Frank Chembiochem Communications A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD(+) into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified. John Wiley and Sons Inc. 2018-10-23 2018-11-16 /pmc/articles/PMC6283237/ /pubmed/30192991 http://dx.doi.org/10.1002/cbic.201800530 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Communications
Höfler, Georg T.
Fernández‐Fueyo, Elena
Pesic, Milja
Younes, Sabry H.
Choi, Eun‐Gyu
Kim, Yong H.
Urlacher, Vlada B.
Arends, Isabel W. C. E.
Hollmann, Frank
A Photoenzymatic NADH Regeneration System
title A Photoenzymatic NADH Regeneration System
title_full A Photoenzymatic NADH Regeneration System
title_fullStr A Photoenzymatic NADH Regeneration System
title_full_unstemmed A Photoenzymatic NADH Regeneration System
title_short A Photoenzymatic NADH Regeneration System
title_sort photoenzymatic nadh regeneration system
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283237/
https://www.ncbi.nlm.nih.gov/pubmed/30192991
http://dx.doi.org/10.1002/cbic.201800530
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