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Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283575/ https://www.ncbi.nlm.nih.gov/pubmed/30521543 http://dx.doi.org/10.1371/journal.pone.0207950 |
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author | Rank, Andreas Nieuwland, Rienk Köhler, Anton Franz, Cordula Waidhauser, Johanna Toth, Bettina |
author_facet | Rank, Andreas Nieuwland, Rienk Köhler, Anton Franz, Cordula Waidhauser, Johanna Toth, Bettina |
author_sort | Rank, Andreas |
collection | PubMed |
description | INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB. METHODS: EV in BM and PB from 12 healthy stem cell donors were measured by flow-cytometry using Annexin V and cell-specific antibodies for hematopoietic stem cells, leucocytes, platelets, red blood cells, and endothelial cells. Additionally, concentrations of tissue factor-bearing EV were evaluated. RESULTS: High numbers of total EV were present in BM (median value [25–75 percentile]: 14.8 x10(9)/l [8.5–19.3]). Non-significantly lower numbers of total EV were measured in PB (9.2 x10(9)/l [3.8–14.5]). However, distribuation of EV subtypes showed substantial differences between BM and PB: In PB, distribution of EV fractions was similar as previously described. Most EV originated from platelets (93.9%), and only few EV were derived from leucocytes (4.5%), erythrocytes (1.8%), endothelial cells (1.0%), and hematopoietic stem cells (0.7%). In contrast, major fractions of BM-EV were derived from red blood cells or erythropoietic cells (43.2%), followed by megacaryocytes / platelets (27.6%), and by leucocytes as well as their progenitor cells (25,7%); only low EV proportions originated from endothelial cells and hematopoietic stem cells (2.0% and 1.5%, respectively). Similar fractions of tissue factor—bearing EV were found in BM and PB (1.3% and 0.9%). CONCULSION: Taken together, we describe EV numbers and their subtype distribution in the BM compartment for the first time. The tissue specific EV distribution reflects BM cell composition and favours the idea of a BM–PB barrier existing not only for cells, but also for EV. |
format | Online Article Text |
id | pubmed-6283575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62835752018-12-20 Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution Rank, Andreas Nieuwland, Rienk Köhler, Anton Franz, Cordula Waidhauser, Johanna Toth, Bettina PLoS One Research Article INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB. METHODS: EV in BM and PB from 12 healthy stem cell donors were measured by flow-cytometry using Annexin V and cell-specific antibodies for hematopoietic stem cells, leucocytes, platelets, red blood cells, and endothelial cells. Additionally, concentrations of tissue factor-bearing EV were evaluated. RESULTS: High numbers of total EV were present in BM (median value [25–75 percentile]: 14.8 x10(9)/l [8.5–19.3]). Non-significantly lower numbers of total EV were measured in PB (9.2 x10(9)/l [3.8–14.5]). However, distribuation of EV subtypes showed substantial differences between BM and PB: In PB, distribution of EV fractions was similar as previously described. Most EV originated from platelets (93.9%), and only few EV were derived from leucocytes (4.5%), erythrocytes (1.8%), endothelial cells (1.0%), and hematopoietic stem cells (0.7%). In contrast, major fractions of BM-EV were derived from red blood cells or erythropoietic cells (43.2%), followed by megacaryocytes / platelets (27.6%), and by leucocytes as well as their progenitor cells (25,7%); only low EV proportions originated from endothelial cells and hematopoietic stem cells (2.0% and 1.5%, respectively). Similar fractions of tissue factor—bearing EV were found in BM and PB (1.3% and 0.9%). CONCULSION: Taken together, we describe EV numbers and their subtype distribution in the BM compartment for the first time. The tissue specific EV distribution reflects BM cell composition and favours the idea of a BM–PB barrier existing not only for cells, but also for EV. Public Library of Science 2018-12-06 /pmc/articles/PMC6283575/ /pubmed/30521543 http://dx.doi.org/10.1371/journal.pone.0207950 Text en © 2018 Rank et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rank, Andreas Nieuwland, Rienk Köhler, Anton Franz, Cordula Waidhauser, Johanna Toth, Bettina Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title | Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title_full | Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title_fullStr | Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title_full_unstemmed | Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title_short | Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
title_sort | human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283575/ https://www.ncbi.nlm.nih.gov/pubmed/30521543 http://dx.doi.org/10.1371/journal.pone.0207950 |
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