Cargando…

Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution

INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However...

Descripción completa

Detalles Bibliográficos
Autores principales: Rank, Andreas, Nieuwland, Rienk, Köhler, Anton, Franz, Cordula, Waidhauser, Johanna, Toth, Bettina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283575/
https://www.ncbi.nlm.nih.gov/pubmed/30521543
http://dx.doi.org/10.1371/journal.pone.0207950
_version_ 1783379186310709248
author Rank, Andreas
Nieuwland, Rienk
Köhler, Anton
Franz, Cordula
Waidhauser, Johanna
Toth, Bettina
author_facet Rank, Andreas
Nieuwland, Rienk
Köhler, Anton
Franz, Cordula
Waidhauser, Johanna
Toth, Bettina
author_sort Rank, Andreas
collection PubMed
description INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB. METHODS: EV in BM and PB from 12 healthy stem cell donors were measured by flow-cytometry using Annexin V and cell-specific antibodies for hematopoietic stem cells, leucocytes, platelets, red blood cells, and endothelial cells. Additionally, concentrations of tissue factor-bearing EV were evaluated. RESULTS: High numbers of total EV were present in BM (median value [25–75 percentile]: 14.8 x10(9)/l [8.5–19.3]). Non-significantly lower numbers of total EV were measured in PB (9.2 x10(9)/l [3.8–14.5]). However, distribuation of EV subtypes showed substantial differences between BM and PB: In PB, distribution of EV fractions was similar as previously described. Most EV originated from platelets (93.9%), and only few EV were derived from leucocytes (4.5%), erythrocytes (1.8%), endothelial cells (1.0%), and hematopoietic stem cells (0.7%). In contrast, major fractions of BM-EV were derived from red blood cells or erythropoietic cells (43.2%), followed by megacaryocytes / platelets (27.6%), and by leucocytes as well as their progenitor cells (25,7%); only low EV proportions originated from endothelial cells and hematopoietic stem cells (2.0% and 1.5%, respectively). Similar fractions of tissue factor—bearing EV were found in BM and PB (1.3% and 0.9%). CONCULSION: Taken together, we describe EV numbers and their subtype distribution in the BM compartment for the first time. The tissue specific EV distribution reflects BM cell composition and favours the idea of a BM–PB barrier existing not only for cells, but also for EV.
format Online
Article
Text
id pubmed-6283575
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-62835752018-12-20 Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution Rank, Andreas Nieuwland, Rienk Köhler, Anton Franz, Cordula Waidhauser, Johanna Toth, Bettina PLoS One Research Article INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB. METHODS: EV in BM and PB from 12 healthy stem cell donors were measured by flow-cytometry using Annexin V and cell-specific antibodies for hematopoietic stem cells, leucocytes, platelets, red blood cells, and endothelial cells. Additionally, concentrations of tissue factor-bearing EV were evaluated. RESULTS: High numbers of total EV were present in BM (median value [25–75 percentile]: 14.8 x10(9)/l [8.5–19.3]). Non-significantly lower numbers of total EV were measured in PB (9.2 x10(9)/l [3.8–14.5]). However, distribuation of EV subtypes showed substantial differences between BM and PB: In PB, distribution of EV fractions was similar as previously described. Most EV originated from platelets (93.9%), and only few EV were derived from leucocytes (4.5%), erythrocytes (1.8%), endothelial cells (1.0%), and hematopoietic stem cells (0.7%). In contrast, major fractions of BM-EV were derived from red blood cells or erythropoietic cells (43.2%), followed by megacaryocytes / platelets (27.6%), and by leucocytes as well as their progenitor cells (25,7%); only low EV proportions originated from endothelial cells and hematopoietic stem cells (2.0% and 1.5%, respectively). Similar fractions of tissue factor—bearing EV were found in BM and PB (1.3% and 0.9%). CONCULSION: Taken together, we describe EV numbers and their subtype distribution in the BM compartment for the first time. The tissue specific EV distribution reflects BM cell composition and favours the idea of a BM–PB barrier existing not only for cells, but also for EV. Public Library of Science 2018-12-06 /pmc/articles/PMC6283575/ /pubmed/30521543 http://dx.doi.org/10.1371/journal.pone.0207950 Text en © 2018 Rank et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rank, Andreas
Nieuwland, Rienk
Köhler, Anton
Franz, Cordula
Waidhauser, Johanna
Toth, Bettina
Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title_full Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title_fullStr Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title_full_unstemmed Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title_short Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
title_sort human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283575/
https://www.ncbi.nlm.nih.gov/pubmed/30521543
http://dx.doi.org/10.1371/journal.pone.0207950
work_keys_str_mv AT rankandreas humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution
AT nieuwlandrienk humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution
AT kohleranton humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution
AT franzcordula humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution
AT waidhauserjohanna humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution
AT tothbettina humanbonemarrowcontainshighlevelsofextracellularvesicleswithatissuespecificsubtypedistribution