Cargando…
Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay
No-go Decay (NGD) is a process that has evolved to deal with stalled ribosomes resulting from structural blocks or aberrant mRNAs. The process is distinguished by an endonucleolytic cleavage prior to degradation of the transcript. While many of the details of the pathway have been described, the ide...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283612/ https://www.ncbi.nlm.nih.gov/pubmed/30475795 http://dx.doi.org/10.1371/journal.pgen.1007818 |
_version_ | 1783379194996064256 |
---|---|
author | Simms, Carrie L. Kim, Kyusik Q. Yan, Liewei L. Qiu, Jessica Zaher, Hani S. |
author_facet | Simms, Carrie L. Kim, Kyusik Q. Yan, Liewei L. Qiu, Jessica Zaher, Hani S. |
author_sort | Simms, Carrie L. |
collection | PubMed |
description | No-go Decay (NGD) is a process that has evolved to deal with stalled ribosomes resulting from structural blocks or aberrant mRNAs. The process is distinguished by an endonucleolytic cleavage prior to degradation of the transcript. While many of the details of the pathway have been described, the identity of the endonuclease remains unknown. Here we identify residues of the small subunit ribosomal protein Rps3 that are important for NGD by affecting the cleavage reaction. Mutation of residues within the ribosomal entry tunnel that contact the incoming mRNA leads to significantly reduced accumulation of cleavage products, independent of the type of stall sequence, and renders cells sensitive to damaging agents thought to trigger NGD. These phenotypes are distinct from those seen in combination with other NGD factors, suggesting a separate role for Rps3 in NGD. Conversely, ribosomal proteins ubiquitination is not affected by rps3 mutations, indicating that upstream ribosome quality control (RQC) events are not dependent on these residues. Together, these results suggest that Rps3 is important for quality control on the ribosome and strongly supports the notion that the ribosome itself plays a central role in the endonucleolytic cleavage reaction during NGD. |
format | Online Article Text |
id | pubmed-6283612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62836122018-12-19 Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay Simms, Carrie L. Kim, Kyusik Q. Yan, Liewei L. Qiu, Jessica Zaher, Hani S. PLoS Genet Research Article No-go Decay (NGD) is a process that has evolved to deal with stalled ribosomes resulting from structural blocks or aberrant mRNAs. The process is distinguished by an endonucleolytic cleavage prior to degradation of the transcript. While many of the details of the pathway have been described, the identity of the endonuclease remains unknown. Here we identify residues of the small subunit ribosomal protein Rps3 that are important for NGD by affecting the cleavage reaction. Mutation of residues within the ribosomal entry tunnel that contact the incoming mRNA leads to significantly reduced accumulation of cleavage products, independent of the type of stall sequence, and renders cells sensitive to damaging agents thought to trigger NGD. These phenotypes are distinct from those seen in combination with other NGD factors, suggesting a separate role for Rps3 in NGD. Conversely, ribosomal proteins ubiquitination is not affected by rps3 mutations, indicating that upstream ribosome quality control (RQC) events are not dependent on these residues. Together, these results suggest that Rps3 is important for quality control on the ribosome and strongly supports the notion that the ribosome itself plays a central role in the endonucleolytic cleavage reaction during NGD. Public Library of Science 2018-11-26 /pmc/articles/PMC6283612/ /pubmed/30475795 http://dx.doi.org/10.1371/journal.pgen.1007818 Text en © 2018 Simms et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Simms, Carrie L. Kim, Kyusik Q. Yan, Liewei L. Qiu, Jessica Zaher, Hani S. Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title | Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title_full | Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title_fullStr | Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title_full_unstemmed | Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title_short | Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
title_sort | interactions between the mrna and rps3/us3 at the entry tunnel of the ribosomal small subunit are important for no-go decay |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6283612/ https://www.ncbi.nlm.nih.gov/pubmed/30475795 http://dx.doi.org/10.1371/journal.pgen.1007818 |
work_keys_str_mv | AT simmscarriel interactionsbetweenthemrnaandrps3us3attheentrytunneloftheribosomalsmallsubunitareimportantfornogodecay AT kimkyusikq interactionsbetweenthemrnaandrps3us3attheentrytunneloftheribosomalsmallsubunitareimportantfornogodecay AT yanlieweil interactionsbetweenthemrnaandrps3us3attheentrytunneloftheribosomalsmallsubunitareimportantfornogodecay AT qiujessica interactionsbetweenthemrnaandrps3us3attheentrytunneloftheribosomalsmallsubunitareimportantfornogodecay AT zaherhanis interactionsbetweenthemrnaandrps3us3attheentrytunneloftheribosomalsmallsubunitareimportantfornogodecay |