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Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression

BACKGROUND: Cancer treatment is based on tumor staging. Curative intent is only applied to localized tumors. Recent studies show that oligometastatic patients who have limited number of metastases may benefit from metastasis-directed local treatments to achieve long-term survival. However, mechanism...

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Autores principales: Sun, Zhiwei, Zhou, Shixia, Tang, Junling, Ye, Ting, Li, Jingyuan, Liu, Doudou, Zhou, Jian, Wang, Jianyu, Rosie Xing, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6284370/
https://www.ncbi.nlm.nih.gov/pubmed/30301603
http://dx.doi.org/10.1016/j.ebiom.2018.10.002
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author Sun, Zhiwei
Zhou, Shixia
Tang, Junling
Ye, Ting
Li, Jingyuan
Liu, Doudou
Zhou, Jian
Wang, Jianyu
Rosie Xing, H.
author_facet Sun, Zhiwei
Zhou, Shixia
Tang, Junling
Ye, Ting
Li, Jingyuan
Liu, Doudou
Zhou, Jian
Wang, Jianyu
Rosie Xing, H.
author_sort Sun, Zhiwei
collection PubMed
description BACKGROUND: Cancer treatment is based on tumor staging. Curative intent is only applied to localized tumors. Recent studies show that oligometastatic patients who have limited number of metastases may benefit from metastasis-directed local treatments to achieve long-term survival. However, mechanisms underlying oligometastatic to polymetastatic progression remains elusive. METHODS: The effects of miR-200c and Sec23a on tumor metastasis were verified both in vitro and in vivo. The secretome changes were detected by mass spectrometry. FINDINGS: We established a pair of homologous lung-metastasis derived oligometastatic and polymetastatic cell lines from human melanoma cancer cell line M14. Using the two cell lines, we have identified Sec23a, a gene target of miR-200c, suppresses miR-200c augmented oligometastatic to polymetastatic progression via its secretome. Firstly, miR-200c over-expression and Sec23a interference accelerated oligometastatic to polymetatic progression. Secondly, Sec23a functions downstream of miR-200c. Thirdly, mass spectrometric analysis of the secretory protein profile suggests that Sec23a-dependent secretome may impact metastatic colonization by modifying tumor microenvironment. Fourthly, the survival analysis using The Cancer Genome Atlas database shows Sec23a as a favorable prognostic marker for skin cutaneous melanoma, supporting the clinical relevance of our findings. INTERPRETATION: The finding that Sec23a is a suppressor of oligometastatic to polymetastatic progression has clinical implications. First, it provides a new theoretical framework for the development of treatments that prevent oligometastasis to polymetastasis. Second, Sec23a may be used as a favorable prognostic marker for the selection of patients with stable oligometastatic disease for oligometastasis-based local therapies of curative intent. FUND: National Natural Science Foundations of China.
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spelling pubmed-62843702018-12-13 Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression Sun, Zhiwei Zhou, Shixia Tang, Junling Ye, Ting Li, Jingyuan Liu, Doudou Zhou, Jian Wang, Jianyu Rosie Xing, H. EBioMedicine Research paper BACKGROUND: Cancer treatment is based on tumor staging. Curative intent is only applied to localized tumors. Recent studies show that oligometastatic patients who have limited number of metastases may benefit from metastasis-directed local treatments to achieve long-term survival. However, mechanisms underlying oligometastatic to polymetastatic progression remains elusive. METHODS: The effects of miR-200c and Sec23a on tumor metastasis were verified both in vitro and in vivo. The secretome changes were detected by mass spectrometry. FINDINGS: We established a pair of homologous lung-metastasis derived oligometastatic and polymetastatic cell lines from human melanoma cancer cell line M14. Using the two cell lines, we have identified Sec23a, a gene target of miR-200c, suppresses miR-200c augmented oligometastatic to polymetastatic progression via its secretome. Firstly, miR-200c over-expression and Sec23a interference accelerated oligometastatic to polymetatic progression. Secondly, Sec23a functions downstream of miR-200c. Thirdly, mass spectrometric analysis of the secretory protein profile suggests that Sec23a-dependent secretome may impact metastatic colonization by modifying tumor microenvironment. Fourthly, the survival analysis using The Cancer Genome Atlas database shows Sec23a as a favorable prognostic marker for skin cutaneous melanoma, supporting the clinical relevance of our findings. INTERPRETATION: The finding that Sec23a is a suppressor of oligometastatic to polymetastatic progression has clinical implications. First, it provides a new theoretical framework for the development of treatments that prevent oligometastasis to polymetastasis. Second, Sec23a may be used as a favorable prognostic marker for the selection of patients with stable oligometastatic disease for oligometastasis-based local therapies of curative intent. FUND: National Natural Science Foundations of China. Elsevier 2018-10-06 /pmc/articles/PMC6284370/ /pubmed/30301603 http://dx.doi.org/10.1016/j.ebiom.2018.10.002 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research paper
Sun, Zhiwei
Zhou, Shixia
Tang, Junling
Ye, Ting
Li, Jingyuan
Liu, Doudou
Zhou, Jian
Wang, Jianyu
Rosie Xing, H.
Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title_full Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title_fullStr Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title_full_unstemmed Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title_short Sec23a mediates miR-200c augmented oligometastatic to polymetastatic progression
title_sort sec23a mediates mir-200c augmented oligometastatic to polymetastatic progression
topic Research paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6284370/
https://www.ncbi.nlm.nih.gov/pubmed/30301603
http://dx.doi.org/10.1016/j.ebiom.2018.10.002
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