The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)

Gsdf is a key gene for testicular differentiation in teleost. However, little is known about the function of Gsdf in Chinese tongue sole (Cynoglossus semilaevis). In this study, we obtained the full-length Gsdf gene (CS-Gsdf), and functional characterization revealed its potential participation duri...

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Autores principales: Zhu, Ying, Meng, Liang, Xu, Wenteng, Cui, Zhongkai, Zhang, Nianwei, Guo, Hua, Wang, Na, Shao, Changwei, Chen, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286346/
https://www.ncbi.nlm.nih.gov/pubmed/30531973
http://dx.doi.org/10.1038/s41598-018-35553-7
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author Zhu, Ying
Meng, Liang
Xu, Wenteng
Cui, Zhongkai
Zhang, Nianwei
Guo, Hua
Wang, Na
Shao, Changwei
Chen, Songlin
author_facet Zhu, Ying
Meng, Liang
Xu, Wenteng
Cui, Zhongkai
Zhang, Nianwei
Guo, Hua
Wang, Na
Shao, Changwei
Chen, Songlin
author_sort Zhu, Ying
collection PubMed
description Gsdf is a key gene for testicular differentiation in teleost. However, little is known about the function of Gsdf in Chinese tongue sole (Cynoglossus semilaevis). In this study, we obtained the full-length Gsdf gene (CS-Gsdf), and functional characterization revealed its potential participation during germ cell differentiation in testes. CS-Gsdf transcription was predominantly detected in gonads, while the levels in testes were significantly higher than those in ovaries. During the different developmental stages in male gonads, the mRNA level was significantly upregulated at 86 dph, and a peak appeared at 120 dph; then, the level decreased at 1 and 2 yph. In situ hybridization revealed that CS-Gsdf mRNA was mainly localized in the Sertoli cells, spermatogonia, and spermatids in mature testes. After CS-Gsdf knockdown in the male testes cell line by RNA interference, a series of sex-related genes was influenced, including several sex differentiation genes, CS-Wnt4a, CS-Cyp19a1a and CS-Star. Based on these data, we speculated that CS-Gsdf may play a positive role in germ differentiation and proliferation via influencing genes related to sex differentiation.
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spelling pubmed-62863462018-12-19 The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis) Zhu, Ying Meng, Liang Xu, Wenteng Cui, Zhongkai Zhang, Nianwei Guo, Hua Wang, Na Shao, Changwei Chen, Songlin Sci Rep Article Gsdf is a key gene for testicular differentiation in teleost. However, little is known about the function of Gsdf in Chinese tongue sole (Cynoglossus semilaevis). In this study, we obtained the full-length Gsdf gene (CS-Gsdf), and functional characterization revealed its potential participation during germ cell differentiation in testes. CS-Gsdf transcription was predominantly detected in gonads, while the levels in testes were significantly higher than those in ovaries. During the different developmental stages in male gonads, the mRNA level was significantly upregulated at 86 dph, and a peak appeared at 120 dph; then, the level decreased at 1 and 2 yph. In situ hybridization revealed that CS-Gsdf mRNA was mainly localized in the Sertoli cells, spermatogonia, and spermatids in mature testes. After CS-Gsdf knockdown in the male testes cell line by RNA interference, a series of sex-related genes was influenced, including several sex differentiation genes, CS-Wnt4a, CS-Cyp19a1a and CS-Star. Based on these data, we speculated that CS-Gsdf may play a positive role in germ differentiation and proliferation via influencing genes related to sex differentiation. Nature Publishing Group UK 2018-12-07 /pmc/articles/PMC6286346/ /pubmed/30531973 http://dx.doi.org/10.1038/s41598-018-35553-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhu, Ying
Meng, Liang
Xu, Wenteng
Cui, Zhongkai
Zhang, Nianwei
Guo, Hua
Wang, Na
Shao, Changwei
Chen, Songlin
The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title_full The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title_fullStr The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title_full_unstemmed The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title_short The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis)
title_sort autosomal gsdf gene plays a role in male gonad development in chinese tongue sole (cynoglossus semilaevis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286346/
https://www.ncbi.nlm.nih.gov/pubmed/30531973
http://dx.doi.org/10.1038/s41598-018-35553-7
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