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Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites

Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied h...

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Autores principales: Taware, Ravindra, Taunk, Khushman, Pereira, Jorge A. M., Shirolkar, Amey, Soneji, Dharmesh, Câmara, José S., Nagarajaram, H. A., Rapole, Srikanth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286361/
https://www.ncbi.nlm.nih.gov/pubmed/30531924
http://dx.doi.org/10.1038/s41598-018-35854-x
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author Taware, Ravindra
Taunk, Khushman
Pereira, Jorge A. M.
Shirolkar, Amey
Soneji, Dharmesh
Câmara, José S.
Nagarajaram, H. A.
Rapole, Srikanth
author_facet Taware, Ravindra
Taunk, Khushman
Pereira, Jorge A. M.
Shirolkar, Amey
Soneji, Dharmesh
Câmara, José S.
Nagarajaram, H. A.
Rapole, Srikanth
author_sort Taware, Ravindra
collection PubMed
description Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC − 32, Control − 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log(2) fold change (log(2) FC) value of ≥0.58/≤−0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future.
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spelling pubmed-62863612018-12-19 Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites Taware, Ravindra Taunk, Khushman Pereira, Jorge A. M. Shirolkar, Amey Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth Sci Rep Article Head and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC − 32, Control − 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log(2) fold change (log(2) FC) value of ≥0.58/≤−0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future. Nature Publishing Group UK 2018-12-07 /pmc/articles/PMC6286361/ /pubmed/30531924 http://dx.doi.org/10.1038/s41598-018-35854-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Taware, Ravindra
Taunk, Khushman
Pereira, Jorge A. M.
Shirolkar, Amey
Soneji, Dharmesh
Câmara, José S.
Nagarajaram, H. A.
Rapole, Srikanth
Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title_full Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title_fullStr Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title_full_unstemmed Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title_short Volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
title_sort volatilomic insight of head and neck cancer via the effects observed on saliva metabolites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286361/
https://www.ncbi.nlm.nih.gov/pubmed/30531924
http://dx.doi.org/10.1038/s41598-018-35854-x
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