Cargando…
Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.)
BACKGROUND: Many large-effect quantitative trait loci (QTLs) for yield and disease resistance related traits have been identified in different mapping populations of peanut (Arachis hypogaea L.) under multiple environments. However, only a limited number of QTLs have been used in marker-assisted sel...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286586/ https://www.ncbi.nlm.nih.gov/pubmed/30526476 http://dx.doi.org/10.1186/s12864-018-5288-3 |
_version_ | 1783379487689277440 |
---|---|
author | Lu, Qing Liu, Hao Hong, Yanbin Li, Haifen Liu, Haiyan Li, Xingyu Wen, Shijie Zhou, Guiyuan Li, Shaoxiong Chen, Xiaoping Liang, Xuanqiang |
author_facet | Lu, Qing Liu, Hao Hong, Yanbin Li, Haifen Liu, Haiyan Li, Xingyu Wen, Shijie Zhou, Guiyuan Li, Shaoxiong Chen, Xiaoping Liang, Xuanqiang |
author_sort | Lu, Qing |
collection | PubMed |
description | BACKGROUND: Many large-effect quantitative trait loci (QTLs) for yield and disease resistance related traits have been identified in different mapping populations of peanut (Arachis hypogaea L.) under multiple environments. However, only a limited number of QTLs have been used in marker-assisted selection (MAS) because of unfavorable epistatic interactions between QTLs in different genetic backgrounds. Thus, it is essential to identify consensus QTLs across different environments and genetic backgrounds for use in MAS. Here, we used QTL meta-analysis to identify a set of consensus QTLs for yield and disease resistance related traits in peanut. RESULTS: A new integrated consensus genetic map with 5874 loci was constructed. The map comprised 20 linkage groups (LGs) and was up to a total length of 2918.62 cM with average marker density of 2.01 loci per centimorgan (cM). A total of 292 initial QTLs were projected on the new consensus map, and 40 meta-QTLs (MQTLs) for yield and disease resistance related traits were detected on four LGs. The genetic intervals of these consensus MQTLs varied from 0.20 cM to 7.4 cM, which is narrower than the genetic intervals of the initial QTLs, meaning they may be suitable for use in MAS. Importantly, a region of the map that previously co-localized multiple major QTLs for pod traits was narrowed from 3.7 cM to 0.7 cM using an overlap region of four MQTLs for yield related traits on LG A05, which corresponds to a physical region of about 630.3 kb on the A05 pseudomolecule of peanut, including 38 annotated candidate genes (54 transcripts) related to catalytic activity and metabolic process. Additionally, one major MQTL for late leaf spot (LLS) was identified in a region of about 0.38 cM. BLAST searches identified 26 candidate genes (30 different transcripts) in this region, some of which were annotated as related to regulation of disease resistance in different plant species. CONCLUSIONS: Combined with the high-density marker consensus map, all the detected MQTLs could be useful in MAS. The biological functions of the 64 candidate genes should be validated to unravel the molecular mechanisms of yield and disease resistance in peanut. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5288-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6286586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62865862018-12-14 Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) Lu, Qing Liu, Hao Hong, Yanbin Li, Haifen Liu, Haiyan Li, Xingyu Wen, Shijie Zhou, Guiyuan Li, Shaoxiong Chen, Xiaoping Liang, Xuanqiang BMC Genomics Research Article BACKGROUND: Many large-effect quantitative trait loci (QTLs) for yield and disease resistance related traits have been identified in different mapping populations of peanut (Arachis hypogaea L.) under multiple environments. However, only a limited number of QTLs have been used in marker-assisted selection (MAS) because of unfavorable epistatic interactions between QTLs in different genetic backgrounds. Thus, it is essential to identify consensus QTLs across different environments and genetic backgrounds for use in MAS. Here, we used QTL meta-analysis to identify a set of consensus QTLs for yield and disease resistance related traits in peanut. RESULTS: A new integrated consensus genetic map with 5874 loci was constructed. The map comprised 20 linkage groups (LGs) and was up to a total length of 2918.62 cM with average marker density of 2.01 loci per centimorgan (cM). A total of 292 initial QTLs were projected on the new consensus map, and 40 meta-QTLs (MQTLs) for yield and disease resistance related traits were detected on four LGs. The genetic intervals of these consensus MQTLs varied from 0.20 cM to 7.4 cM, which is narrower than the genetic intervals of the initial QTLs, meaning they may be suitable for use in MAS. Importantly, a region of the map that previously co-localized multiple major QTLs for pod traits was narrowed from 3.7 cM to 0.7 cM using an overlap region of four MQTLs for yield related traits on LG A05, which corresponds to a physical region of about 630.3 kb on the A05 pseudomolecule of peanut, including 38 annotated candidate genes (54 transcripts) related to catalytic activity and metabolic process. Additionally, one major MQTL for late leaf spot (LLS) was identified in a region of about 0.38 cM. BLAST searches identified 26 candidate genes (30 different transcripts) in this region, some of which were annotated as related to regulation of disease resistance in different plant species. CONCLUSIONS: Combined with the high-density marker consensus map, all the detected MQTLs could be useful in MAS. The biological functions of the 64 candidate genes should be validated to unravel the molecular mechanisms of yield and disease resistance in peanut. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5288-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-07 /pmc/articles/PMC6286586/ /pubmed/30526476 http://dx.doi.org/10.1186/s12864-018-5288-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lu, Qing Liu, Hao Hong, Yanbin Li, Haifen Liu, Haiyan Li, Xingyu Wen, Shijie Zhou, Guiyuan Li, Shaoxiong Chen, Xiaoping Liang, Xuanqiang Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title | Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title_full | Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title_fullStr | Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title_full_unstemmed | Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title_short | Consensus map integration and QTL meta-analysis narrowed a locus for yield traits to 0.7 cM and refined a region for late leaf spot resistance traits to 0.38 cM on linkage group A05 in peanut (Arachis hypogaea L.) |
title_sort | consensus map integration and qtl meta-analysis narrowed a locus for yield traits to 0.7 cm and refined a region for late leaf spot resistance traits to 0.38 cm on linkage group a05 in peanut (arachis hypogaea l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286586/ https://www.ncbi.nlm.nih.gov/pubmed/30526476 http://dx.doi.org/10.1186/s12864-018-5288-3 |
work_keys_str_mv | AT luqing consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT liuhao consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT hongyanbin consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT lihaifen consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT liuhaiyan consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT lixingyu consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT wenshijie consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT zhouguiyuan consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT lishaoxiong consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT chenxiaoping consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal AT liangxuanqiang consensusmapintegrationandqtlmetaanalysisnarrowedalocusforyieldtraitsto07cmandrefinedaregionforlateleafspotresistancetraitsto038cmonlinkagegroupa05inpeanutarachishypogaeal |