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OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus
OPCML hypermethylation is considered a promising cancer biomarker. We examined methylation levels in the first exon of OPCML in two patient cohorts within the esophageal adenocarcinoma and gastric adenocarcinoma cascades and in a range of cell-lines using a custom PyroMark CpG assay. Methylation lev...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286609/ https://www.ncbi.nlm.nih.gov/pubmed/30555700 http://dx.doi.org/10.1186/s40364-018-0150-y |
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author | Castaño-Rodríguez, Natalia Popple, Georgia L. Porras-Hurtado, Gloria Liliana Cardona-Deazza, José Luis Montoya-Martinez, Juan José Cadavid-Velez, Antonio Javier Toro-Hidalgo, Héctor William Cobo-Alvarado, Alba Ruth del Socorro Hincapié-Rincón, Ofelia Riordan, Stephen M. Kaakoush, Nadeem O. |
author_facet | Castaño-Rodríguez, Natalia Popple, Georgia L. Porras-Hurtado, Gloria Liliana Cardona-Deazza, José Luis Montoya-Martinez, Juan José Cadavid-Velez, Antonio Javier Toro-Hidalgo, Héctor William Cobo-Alvarado, Alba Ruth del Socorro Hincapié-Rincón, Ofelia Riordan, Stephen M. Kaakoush, Nadeem O. |
author_sort | Castaño-Rodríguez, Natalia |
collection | PubMed |
description | OPCML hypermethylation is considered a promising cancer biomarker. We examined methylation levels in the first exon of OPCML in two patient cohorts within the esophageal adenocarcinoma and gastric adenocarcinoma cascades and in a range of cell-lines using a custom PyroMark CpG assay. Methylation levels were significantly higher in esophageal tissue with histologically confirmed glandular mucosa as compared to tissue from normal esophagi or gastro-esophageal reflux disease. Higher levels of OPCML methylation were absent in the adjacent normal esophageal tissue of patients with glandular mucosa. Higher levels of methylation were confirmed in cell-lines derived from patients with adenocarcinoma, but also detected in two cell-lines with signs of dysplasia. We validated our assay by showing no differences in methylation levels in DNA extracted from blood of patients within the gastric adenocarcinoma cascade. OPCML hypermethylation is present in a subset of patients with metaplastic changes in their esophagus. |
format | Online Article Text |
id | pubmed-6286609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62866092018-12-14 OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus Castaño-Rodríguez, Natalia Popple, Georgia L. Porras-Hurtado, Gloria Liliana Cardona-Deazza, José Luis Montoya-Martinez, Juan José Cadavid-Velez, Antonio Javier Toro-Hidalgo, Héctor William Cobo-Alvarado, Alba Ruth del Socorro Hincapié-Rincón, Ofelia Riordan, Stephen M. Kaakoush, Nadeem O. Biomark Res Short Report OPCML hypermethylation is considered a promising cancer biomarker. We examined methylation levels in the first exon of OPCML in two patient cohorts within the esophageal adenocarcinoma and gastric adenocarcinoma cascades and in a range of cell-lines using a custom PyroMark CpG assay. Methylation levels were significantly higher in esophageal tissue with histologically confirmed glandular mucosa as compared to tissue from normal esophagi or gastro-esophageal reflux disease. Higher levels of OPCML methylation were absent in the adjacent normal esophageal tissue of patients with glandular mucosa. Higher levels of methylation were confirmed in cell-lines derived from patients with adenocarcinoma, but also detected in two cell-lines with signs of dysplasia. We validated our assay by showing no differences in methylation levels in DNA extracted from blood of patients within the gastric adenocarcinoma cascade. OPCML hypermethylation is present in a subset of patients with metaplastic changes in their esophagus. BioMed Central 2018-12-07 /pmc/articles/PMC6286609/ /pubmed/30555700 http://dx.doi.org/10.1186/s40364-018-0150-y Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Castaño-Rodríguez, Natalia Popple, Georgia L. Porras-Hurtado, Gloria Liliana Cardona-Deazza, José Luis Montoya-Martinez, Juan José Cadavid-Velez, Antonio Javier Toro-Hidalgo, Héctor William Cobo-Alvarado, Alba Ruth del Socorro Hincapié-Rincón, Ofelia Riordan, Stephen M. Kaakoush, Nadeem O. OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title_full | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title_fullStr | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title_full_unstemmed | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title_short | OPCML is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
title_sort | opcml is hypermethylated in a subset of patients with metaplastic changes in their esophagus |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286609/ https://www.ncbi.nlm.nih.gov/pubmed/30555700 http://dx.doi.org/10.1186/s40364-018-0150-y |
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