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Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats

Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD(−)). Discussion on the impact of obesity/VD(−) on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD(−) on viability and plasma membrane integ...

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Autores principales: Merino, O., Sánchez, R., Gregorio, B. M., Sampaio, F. J., Risopatrón, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286761/
https://www.ncbi.nlm.nih.gov/pubmed/30596095
http://dx.doi.org/10.1155/2018/5479057
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author Merino, O.
Sánchez, R.
Gregorio, B. M.
Sampaio, F. J.
Risopatrón, J.
author_facet Merino, O.
Sánchez, R.
Gregorio, B. M.
Sampaio, F. J.
Risopatrón, J.
author_sort Merino, O.
collection PubMed
description Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD(−)). Discussion on the impact of obesity/VD(−) on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD(−) on viability and plasma membrane integrity (PMI), superoxide anion (O(2)(−)) level, and DNA fragmentation (DNA(frag)) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD(+)), G2: control diet without vitamin D (C/VD(−)), G3: high-fat diet with vitamin D (HF/VD(+)), and G4: high-fat diet without vitamin D (HF/VD(−)). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O(2)(−) levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD(−) produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.
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spelling pubmed-62867612018-12-30 Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats Merino, O. Sánchez, R. Gregorio, B. M. Sampaio, F. J. Risopatrón, J. Biomed Res Int Research Article Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD(−)). Discussion on the impact of obesity/VD(−) on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD(−) on viability and plasma membrane integrity (PMI), superoxide anion (O(2)(−)) level, and DNA fragmentation (DNA(frag)) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD(+)), G2: control diet without vitamin D (C/VD(−)), G3: high-fat diet with vitamin D (HF/VD(+)), and G4: high-fat diet without vitamin D (HF/VD(−)). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O(2)(−) levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD(−) produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa. Hindawi 2018-11-25 /pmc/articles/PMC6286761/ /pubmed/30596095 http://dx.doi.org/10.1155/2018/5479057 Text en Copyright © 2018 O. Merino et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Merino, O.
Sánchez, R.
Gregorio, B. M.
Sampaio, F. J.
Risopatrón, J.
Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title_full Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title_fullStr Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title_full_unstemmed Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title_short Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
title_sort effects of diet-induced obesity and deficient in vitamin d on spermatozoa function and dna integrity in sprague-dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286761/
https://www.ncbi.nlm.nih.gov/pubmed/30596095
http://dx.doi.org/10.1155/2018/5479057
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