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Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots

Background and Aims: Understanding interactions between water and nitrate fluxes in response to nitrate availability and transpiration rate is crucial to select more efficient plants for the use of water and nitrate. Methods: Some of these interactions were investigated in intact Brassica napus plan...

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Autores principales: Orieux, Charline, Demarest, Gilles, Decau, Marie-Laure, Beauclair, Patrick, Bataillé, Marie-Paule, Le Deunff, Erwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287045/
https://www.ncbi.nlm.nih.gov/pubmed/30559754
http://dx.doi.org/10.3389/fpls.2018.01751
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author Orieux, Charline
Demarest, Gilles
Decau, Marie-Laure
Beauclair, Patrick
Bataillé, Marie-Paule
Le Deunff, Erwan
author_facet Orieux, Charline
Demarest, Gilles
Decau, Marie-Laure
Beauclair, Patrick
Bataillé, Marie-Paule
Le Deunff, Erwan
author_sort Orieux, Charline
collection PubMed
description Background and Aims: Understanding interactions between water and nitrate fluxes in response to nitrate availability and transpiration rate is crucial to select more efficient plants for the use of water and nitrate. Methods: Some of these interactions were investigated in intact Brassica napus plants by combining a non-destructive gravimetric device with (15)NO(3)(-) labeling. The set-up allowed high-resolution measurement of the effects of a cross-combination of two concentrations of KNO(3) or KCl (0.5 and 5 mM) with two different rates of transpiration controlled by the relative humidity during a day–night cycle. Key Results: Results show that (1) high external nitrate concentrations increased root water uptake significantly whatever the transpiration rate, (2) nitrate translocation depended both on the rate of nitrate uptake and loading into xylem (3) dilution-concentration effect of nitrate in the xylem was mainly modulated by both external nitrate availability and transpiration rate, (4) dynamic changes in (15)N translocation in the xylem modified shoot growth and capacitance, and (5) variations in tissue concentrations of NO(3)(-) induced by the experimental conditions were balanced by changes in concentrations of chloride and sulfate ions. These effects were even more amplified under low transpiration condition and 0.5 mM external nitrate concentration. Conclusion: Taken together, these results highlight the fine and rapid adjustment of anion contents, nitrate and water flows to changes in transpiration rate and nitrate availability during a day–night cycle. The use of this non-invasive gravimetric device is therefore a powerful tool to assess candidates genes involved in nitrogen and water use efficiency.
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spelling pubmed-62870452018-12-17 Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots Orieux, Charline Demarest, Gilles Decau, Marie-Laure Beauclair, Patrick Bataillé, Marie-Paule Le Deunff, Erwan Front Plant Sci Plant Science Background and Aims: Understanding interactions between water and nitrate fluxes in response to nitrate availability and transpiration rate is crucial to select more efficient plants for the use of water and nitrate. Methods: Some of these interactions were investigated in intact Brassica napus plants by combining a non-destructive gravimetric device with (15)NO(3)(-) labeling. The set-up allowed high-resolution measurement of the effects of a cross-combination of two concentrations of KNO(3) or KCl (0.5 and 5 mM) with two different rates of transpiration controlled by the relative humidity during a day–night cycle. Key Results: Results show that (1) high external nitrate concentrations increased root water uptake significantly whatever the transpiration rate, (2) nitrate translocation depended both on the rate of nitrate uptake and loading into xylem (3) dilution-concentration effect of nitrate in the xylem was mainly modulated by both external nitrate availability and transpiration rate, (4) dynamic changes in (15)N translocation in the xylem modified shoot growth and capacitance, and (5) variations in tissue concentrations of NO(3)(-) induced by the experimental conditions were balanced by changes in concentrations of chloride and sulfate ions. These effects were even more amplified under low transpiration condition and 0.5 mM external nitrate concentration. Conclusion: Taken together, these results highlight the fine and rapid adjustment of anion contents, nitrate and water flows to changes in transpiration rate and nitrate availability during a day–night cycle. The use of this non-invasive gravimetric device is therefore a powerful tool to assess candidates genes involved in nitrogen and water use efficiency. Frontiers Media S.A. 2018-12-03 /pmc/articles/PMC6287045/ /pubmed/30559754 http://dx.doi.org/10.3389/fpls.2018.01751 Text en Copyright © 2018 Orieux, Demarest, Decau, Beauclair, Bataillé and Le Deunff. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Orieux, Charline
Demarest, Gilles
Decau, Marie-Laure
Beauclair, Patrick
Bataillé, Marie-Paule
Le Deunff, Erwan
Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title_full Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title_fullStr Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title_full_unstemmed Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title_short Changes in (15)NO(3)(-) Availability and Transpiration Rate Are Associated With a Rapid Diurnal Adjustment of Anion Contents as Well as (15)N and Water Fluxes Between the Roots and Shoots
title_sort changes in (15)no(3)(-) availability and transpiration rate are associated with a rapid diurnal adjustment of anion contents as well as (15)n and water fluxes between the roots and shoots
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287045/
https://www.ncbi.nlm.nih.gov/pubmed/30559754
http://dx.doi.org/10.3389/fpls.2018.01751
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