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Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion

PURPOSE: Negative elongation factor complex member C/D (NELFCD), mapped to chromosome 20q13.32, has been found to be significantly overexpressed in colorectal cancer (CRC) by our previous research. However, whether its overexpression contributes to CRC development is unknown. We aimed to explore the...

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Autores principales: Song, Shenglei, Li, Daojiang, Yang, Chunxing, Yan, Peicheng, Bai, Yang, Zhang, Yi, Hu, Gui, Lin, Changwei, Li, Xiaorong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287418/
https://www.ncbi.nlm.nih.gov/pubmed/30584332
http://dx.doi.org/10.2147/OTT.S186266
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author Song, Shenglei
Li, Daojiang
Yang, Chunxing
Yan, Peicheng
Bai, Yang
Zhang, Yi
Hu, Gui
Lin, Changwei
Li, Xiaorong
author_facet Song, Shenglei
Li, Daojiang
Yang, Chunxing
Yan, Peicheng
Bai, Yang
Zhang, Yi
Hu, Gui
Lin, Changwei
Li, Xiaorong
author_sort Song, Shenglei
collection PubMed
description PURPOSE: Negative elongation factor complex member C/D (NELFCD), mapped to chromosome 20q13.32, has been found to be significantly overexpressed in colorectal cancer (CRC) by our previous research. However, whether its overexpression contributes to CRC development is unknown. We aimed to explore the biological and clinical roles of NELFCD in CRC. MATERIALS AND METHODS: The expression of NELFCD was detected by qRT-PCR and Western blot. The biological function of NELFCD on CRC cell proliferation, migration, invasion, and apoptosis was detected by cell counting kit-8, plate colony formation assay, transwell migration and invasion assays, and flow cytometry in vitro and by murine xenograft tumor growth in vivo. Moreover, we evaluated the correction between its expression level and clinicopathologic parameters. RESULTS: We found NELFCD was overexpressed in 50 pairs of CRC tissues in comparison to the adjacent nontumor tissues (P<0.05). Knockdown of NELFCD significantly impaired cell proliferation, migration and invasion abilities, facilitated cell apoptosis in vitro, and inhibited tumorigenesis of CRC cells in vivo. NELFCD levels were remarkably connected with tumor location in CRC patients. CONCLUSION: NELFCD is overexpressed and plays an oncogenic role in CRC. Targeting NELFCD may provide a potential therapeutic option for NELFCD-amplified tumors.
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spelling pubmed-62874182018-12-24 Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion Song, Shenglei Li, Daojiang Yang, Chunxing Yan, Peicheng Bai, Yang Zhang, Yi Hu, Gui Lin, Changwei Li, Xiaorong Onco Targets Ther Original Research PURPOSE: Negative elongation factor complex member C/D (NELFCD), mapped to chromosome 20q13.32, has been found to be significantly overexpressed in colorectal cancer (CRC) by our previous research. However, whether its overexpression contributes to CRC development is unknown. We aimed to explore the biological and clinical roles of NELFCD in CRC. MATERIALS AND METHODS: The expression of NELFCD was detected by qRT-PCR and Western blot. The biological function of NELFCD on CRC cell proliferation, migration, invasion, and apoptosis was detected by cell counting kit-8, plate colony formation assay, transwell migration and invasion assays, and flow cytometry in vitro and by murine xenograft tumor growth in vivo. Moreover, we evaluated the correction between its expression level and clinicopathologic parameters. RESULTS: We found NELFCD was overexpressed in 50 pairs of CRC tissues in comparison to the adjacent nontumor tissues (P<0.05). Knockdown of NELFCD significantly impaired cell proliferation, migration and invasion abilities, facilitated cell apoptosis in vitro, and inhibited tumorigenesis of CRC cells in vivo. NELFCD levels were remarkably connected with tumor location in CRC patients. CONCLUSION: NELFCD is overexpressed and plays an oncogenic role in CRC. Targeting NELFCD may provide a potential therapeutic option for NELFCD-amplified tumors. Dove Medical Press 2018-12-05 /pmc/articles/PMC6287418/ /pubmed/30584332 http://dx.doi.org/10.2147/OTT.S186266 Text en © 2018 Song et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Song, Shenglei
Li, Daojiang
Yang, Chunxing
Yan, Peicheng
Bai, Yang
Zhang, Yi
Hu, Gui
Lin, Changwei
Li, Xiaorong
Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title_full Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title_fullStr Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title_full_unstemmed Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title_short Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
title_sort overexpression of nelfcd promotes colorectal cancer cells proliferation, migration, and invasion
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287418/
https://www.ncbi.nlm.nih.gov/pubmed/30584332
http://dx.doi.org/10.2147/OTT.S186266
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