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2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels

A variety of polycyclic pyridines have been proposed as inhibitors of the small conductance calcium-activated potassium (SK) channel. To this group belongs 2,6-bis(2-benzimidazolyl)pyridine (BBP), a commercially and readily available small organic compound which has earlier been described in a broad...

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Autores principales: Simó-Vicens, Rafel, Bomholtz, Sofia H., Sørensen, Ulrik S., Bentzen, Bo H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287599/
https://www.ncbi.nlm.nih.gov/pubmed/30559671
http://dx.doi.org/10.3389/fphar.2018.01409
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author Simó-Vicens, Rafel
Bomholtz, Sofia H.
Sørensen, Ulrik S.
Bentzen, Bo H.
author_facet Simó-Vicens, Rafel
Bomholtz, Sofia H.
Sørensen, Ulrik S.
Bentzen, Bo H.
author_sort Simó-Vicens, Rafel
collection PubMed
description A variety of polycyclic pyridines have been proposed as inhibitors of the small conductance calcium-activated potassium (SK) channel. To this group belongs 2,6-bis(2-benzimidazolyl)pyridine (BBP), a commercially and readily available small organic compound which has earlier been described in a broad range of chemical and biological uses. Here, we show how BBP can also be used as a potent and specific SK channel blocker in vitro. The potency of BBP was measured using automatic patch clamp on all three SK channel subtypes, resulting in similar IC(50) of 0.4 μM. We also assessed the selectivity of BBP on a panel of calcium-activated and voltage-activated potassium channels using two-electrode voltage clamp, automatic and manual patch clamp. BBP did not have any effect on IK, K(ir)2.1, K(ir)3.1+K(ir)3.4, K(v)1.5, K(v)4.3/K(CHIP)2 and K(v)7.1/KCNE1 currents and was 4.8-fold and 46-fold more potent on all SK channel subtypes vs. BK and hERG channels, respectively. Moreover, we were able to identify H491 as a critical amino acid for the pharmacological effect of BBP on the SK channel. From a medicinal chemistry perspective, BBP could be used as a starting point for the design of new and improved SK inhibitors.
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spelling pubmed-62875992018-12-17 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels Simó-Vicens, Rafel Bomholtz, Sofia H. Sørensen, Ulrik S. Bentzen, Bo H. Front Pharmacol Pharmacology A variety of polycyclic pyridines have been proposed as inhibitors of the small conductance calcium-activated potassium (SK) channel. To this group belongs 2,6-bis(2-benzimidazolyl)pyridine (BBP), a commercially and readily available small organic compound which has earlier been described in a broad range of chemical and biological uses. Here, we show how BBP can also be used as a potent and specific SK channel blocker in vitro. The potency of BBP was measured using automatic patch clamp on all three SK channel subtypes, resulting in similar IC(50) of 0.4 μM. We also assessed the selectivity of BBP on a panel of calcium-activated and voltage-activated potassium channels using two-electrode voltage clamp, automatic and manual patch clamp. BBP did not have any effect on IK, K(ir)2.1, K(ir)3.1+K(ir)3.4, K(v)1.5, K(v)4.3/K(CHIP)2 and K(v)7.1/KCNE1 currents and was 4.8-fold and 46-fold more potent on all SK channel subtypes vs. BK and hERG channels, respectively. Moreover, we were able to identify H491 as a critical amino acid for the pharmacological effect of BBP on the SK channel. From a medicinal chemistry perspective, BBP could be used as a starting point for the design of new and improved SK inhibitors. Frontiers Media S.A. 2018-12-03 /pmc/articles/PMC6287599/ /pubmed/30559671 http://dx.doi.org/10.3389/fphar.2018.01409 Text en Copyright © 2018 Simó-Vicens, Bomholtz, Sørensen and Bentzen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Simó-Vicens, Rafel
Bomholtz, Sofia H.
Sørensen, Ulrik S.
Bentzen, Bo H.
2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title_full 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title_fullStr 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title_full_unstemmed 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title_short 2,6-Bis(2-Benzimidazolyl)Pyridine (BBP) Is a Potent and Selective Inhibitor of Small Conductance Calcium-Activated Potassium (SK) Channels
title_sort 2,6-bis(2-benzimidazolyl)pyridine (bbp) is a potent and selective inhibitor of small conductance calcium-activated potassium (sk) channels
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287599/
https://www.ncbi.nlm.nih.gov/pubmed/30559671
http://dx.doi.org/10.3389/fphar.2018.01409
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