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A transcriptomics analysis of the Tbx5 paralogues in zebrafish
TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and ret...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287840/ https://www.ncbi.nlm.nih.gov/pubmed/30532148 http://dx.doi.org/10.1371/journal.pone.0208766 |
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author | Boyle Anderson, Erin A. T. Ho, Robert K. |
author_facet | Boyle Anderson, Erin A. T. Ho, Robert K. |
author_sort | Boyle Anderson, Erin A. T. |
collection | PubMed |
description | TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels. |
format | Online Article Text |
id | pubmed-6287840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62878402018-12-28 A transcriptomics analysis of the Tbx5 paralogues in zebrafish Boyle Anderson, Erin A. T. Ho, Robert K. PLoS One Research Article TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels. Public Library of Science 2018-12-10 /pmc/articles/PMC6287840/ /pubmed/30532148 http://dx.doi.org/10.1371/journal.pone.0208766 Text en © 2018 Boyle Anderson, Ho http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Boyle Anderson, Erin A. T. Ho, Robert K. A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title | A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title_full | A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title_fullStr | A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title_full_unstemmed | A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title_short | A transcriptomics analysis of the Tbx5 paralogues in zebrafish |
title_sort | transcriptomics analysis of the tbx5 paralogues in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287840/ https://www.ncbi.nlm.nih.gov/pubmed/30532148 http://dx.doi.org/10.1371/journal.pone.0208766 |
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