Cargando…

A transcriptomics analysis of the Tbx5 paralogues in zebrafish

TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and ret...

Descripción completa

Detalles Bibliográficos
Autores principales: Boyle Anderson, Erin A. T., Ho, Robert K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287840/
https://www.ncbi.nlm.nih.gov/pubmed/30532148
http://dx.doi.org/10.1371/journal.pone.0208766
_version_ 1783379694685519872
author Boyle Anderson, Erin A. T.
Ho, Robert K.
author_facet Boyle Anderson, Erin A. T.
Ho, Robert K.
author_sort Boyle Anderson, Erin A. T.
collection PubMed
description TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.
format Online
Article
Text
id pubmed-6287840
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-62878402018-12-28 A transcriptomics analysis of the Tbx5 paralogues in zebrafish Boyle Anderson, Erin A. T. Ho, Robert K. PLoS One Research Article TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels. Public Library of Science 2018-12-10 /pmc/articles/PMC6287840/ /pubmed/30532148 http://dx.doi.org/10.1371/journal.pone.0208766 Text en © 2018 Boyle Anderson, Ho http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Boyle Anderson, Erin A. T.
Ho, Robert K.
A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title_full A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title_fullStr A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title_full_unstemmed A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title_short A transcriptomics analysis of the Tbx5 paralogues in zebrafish
title_sort transcriptomics analysis of the tbx5 paralogues in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287840/
https://www.ncbi.nlm.nih.gov/pubmed/30532148
http://dx.doi.org/10.1371/journal.pone.0208766
work_keys_str_mv AT boyleandersonerinat atranscriptomicsanalysisofthetbx5paraloguesinzebrafish
AT horobertk atranscriptomicsanalysisofthetbx5paraloguesinzebrafish
AT boyleandersonerinat transcriptomicsanalysisofthetbx5paraloguesinzebrafish
AT horobertk transcriptomicsanalysisofthetbx5paraloguesinzebrafish