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Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules

Deterministic lateral displacement (DLD) has been extensively implemented in the last decade for size-based sample preparation, owing to its high separation performances for a wide range of particle dimensions. However, separating particles from 1 μm to 10 μm in one single DLD device is challenging...

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Autores principales: Pariset, Eloise, Parent, Charlotte, Fouillet, Yves, François, Boizot, Verplanck, Nicolas, Revol-Cavalier, Frédéric, Thuaire, Aurélie, Agache, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288093/
https://www.ncbi.nlm.nih.gov/pubmed/30531826
http://dx.doi.org/10.1038/s41598-018-34958-8
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author Pariset, Eloise
Parent, Charlotte
Fouillet, Yves
François, Boizot
Verplanck, Nicolas
Revol-Cavalier, Frédéric
Thuaire, Aurélie
Agache, Vincent
author_facet Pariset, Eloise
Parent, Charlotte
Fouillet, Yves
François, Boizot
Verplanck, Nicolas
Revol-Cavalier, Frédéric
Thuaire, Aurélie
Agache, Vincent
author_sort Pariset, Eloise
collection PubMed
description Deterministic lateral displacement (DLD) has been extensively implemented in the last decade for size-based sample preparation, owing to its high separation performances for a wide range of particle dimensions. However, separating particles from 1 μm to 10 μm in one single DLD device is challenging because of the required diversity of pillar dimensions and inherent fabrication issues. This paper presents an alternative approach to achieve the extraction of E. coli bacteria from blood samples spiked with prostate cancer cells. Our approach consists in cascading individual DLD devices in a single automated platform, using flexible chambers that successively collect and inject the sample between each DLD stage without any external sample manipulation. Operating DLD separations independently enables to maximize the sorting efficiency at each step, without any disturbance from downstream stages. The proposed two-step automated protocol is applied to the separation of three types of components (bacteria, blood particles and cancer cells), with a depletion yield of 100% for cancer cells and 93% for red blood cells. This cascaded approach is presented for the first time with two DLD modules and is upscalable to improve the dynamic range of currently available DLD devices.
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spelling pubmed-62880932018-12-19 Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules Pariset, Eloise Parent, Charlotte Fouillet, Yves François, Boizot Verplanck, Nicolas Revol-Cavalier, Frédéric Thuaire, Aurélie Agache, Vincent Sci Rep Article Deterministic lateral displacement (DLD) has been extensively implemented in the last decade for size-based sample preparation, owing to its high separation performances for a wide range of particle dimensions. However, separating particles from 1 μm to 10 μm in one single DLD device is challenging because of the required diversity of pillar dimensions and inherent fabrication issues. This paper presents an alternative approach to achieve the extraction of E. coli bacteria from blood samples spiked with prostate cancer cells. Our approach consists in cascading individual DLD devices in a single automated platform, using flexible chambers that successively collect and inject the sample between each DLD stage without any external sample manipulation. Operating DLD separations independently enables to maximize the sorting efficiency at each step, without any disturbance from downstream stages. The proposed two-step automated protocol is applied to the separation of three types of components (bacteria, blood particles and cancer cells), with a depletion yield of 100% for cancer cells and 93% for red blood cells. This cascaded approach is presented for the first time with two DLD modules and is upscalable to improve the dynamic range of currently available DLD devices. Nature Publishing Group UK 2018-12-10 /pmc/articles/PMC6288093/ /pubmed/30531826 http://dx.doi.org/10.1038/s41598-018-34958-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pariset, Eloise
Parent, Charlotte
Fouillet, Yves
François, Boizot
Verplanck, Nicolas
Revol-Cavalier, Frédéric
Thuaire, Aurélie
Agache, Vincent
Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title_full Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title_fullStr Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title_full_unstemmed Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title_short Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules
title_sort separation of biological particles in a modular platform of cascaded deterministic lateral displacement modules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288093/
https://www.ncbi.nlm.nih.gov/pubmed/30531826
http://dx.doi.org/10.1038/s41598-018-34958-8
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