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Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans

RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within...

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Autores principales: Poush, Julian A., Blouin, Nicolas A., Di Bona, Kristin R., Lažetić, Vladimir, Fay, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288150/
https://www.ncbi.nlm.nih.gov/pubmed/30531803
http://dx.doi.org/10.1038/s41598-018-35691-y
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author Poush, Julian A.
Blouin, Nicolas A.
Di Bona, Kristin R.
Lažetić, Vladimir
Fay, David S.
author_facet Poush, Julian A.
Blouin, Nicolas A.
Di Bona, Kristin R.
Lažetić, Vladimir
Fay, David S.
author_sort Poush, Julian A.
collection PubMed
description RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within the Caenorhabditis clade in recent history, a characteristic shared by some genes involved in germline development. We then examined the effects of deleting all ARI1 family members in the nematode Caenorhabditis elegans, which to our knowledge represents the first complete knockout of ARI1 function in a metazoan. Hermaphrodites that lacked or had strongly reduced ARI1 activity had low fecundity and were partially defective in initiation of oocyte differentiation. We provide evidence that the C. elegans ARI1s likely function downstream or in parallel to FBF-1 and FBF-2, two closely related RNA-binding proteins that are required for the switch from spermatogenesis to oogenesis during late larval development. Previous studies have shown that the E2 enzymes UBC-18/UBCH7 and UBC-3/CDC34 can functionally collaborate with ARI1 family members. Our data indicated that UBC-18, but not UBC-3, specifically cooperates with the ARI1s in germline development. These findings provide new insights into the functions of RING-between-RING proteins and Ariadne E3s during development.
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spelling pubmed-62881502018-12-19 Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans Poush, Julian A. Blouin, Nicolas A. Di Bona, Kristin R. Lažetić, Vladimir Fay, David S. Sci Rep Article RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within the Caenorhabditis clade in recent history, a characteristic shared by some genes involved in germline development. We then examined the effects of deleting all ARI1 family members in the nematode Caenorhabditis elegans, which to our knowledge represents the first complete knockout of ARI1 function in a metazoan. Hermaphrodites that lacked or had strongly reduced ARI1 activity had low fecundity and were partially defective in initiation of oocyte differentiation. We provide evidence that the C. elegans ARI1s likely function downstream or in parallel to FBF-1 and FBF-2, two closely related RNA-binding proteins that are required for the switch from spermatogenesis to oogenesis during late larval development. Previous studies have shown that the E2 enzymes UBC-18/UBCH7 and UBC-3/CDC34 can functionally collaborate with ARI1 family members. Our data indicated that UBC-18, but not UBC-3, specifically cooperates with the ARI1s in germline development. These findings provide new insights into the functions of RING-between-RING proteins and Ariadne E3s during development. Nature Publishing Group UK 2018-12-10 /pmc/articles/PMC6288150/ /pubmed/30531803 http://dx.doi.org/10.1038/s41598-018-35691-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Poush, Julian A.
Blouin, Nicolas A.
Di Bona, Kristin R.
Lažetić, Vladimir
Fay, David S.
Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title_full Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title_fullStr Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title_full_unstemmed Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title_short Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
title_sort regulation of germ cell development by ari1 family ubiquitin ligases in c. elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288150/
https://www.ncbi.nlm.nih.gov/pubmed/30531803
http://dx.doi.org/10.1038/s41598-018-35691-y
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