Cargando…
Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans
RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288150/ https://www.ncbi.nlm.nih.gov/pubmed/30531803 http://dx.doi.org/10.1038/s41598-018-35691-y |
_version_ | 1783379741956374528 |
---|---|
author | Poush, Julian A. Blouin, Nicolas A. Di Bona, Kristin R. Lažetić, Vladimir Fay, David S. |
author_facet | Poush, Julian A. Blouin, Nicolas A. Di Bona, Kristin R. Lažetić, Vladimir Fay, David S. |
author_sort | Poush, Julian A. |
collection | PubMed |
description | RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within the Caenorhabditis clade in recent history, a characteristic shared by some genes involved in germline development. We then examined the effects of deleting all ARI1 family members in the nematode Caenorhabditis elegans, which to our knowledge represents the first complete knockout of ARI1 function in a metazoan. Hermaphrodites that lacked or had strongly reduced ARI1 activity had low fecundity and were partially defective in initiation of oocyte differentiation. We provide evidence that the C. elegans ARI1s likely function downstream or in parallel to FBF-1 and FBF-2, two closely related RNA-binding proteins that are required for the switch from spermatogenesis to oogenesis during late larval development. Previous studies have shown that the E2 enzymes UBC-18/UBCH7 and UBC-3/CDC34 can functionally collaborate with ARI1 family members. Our data indicated that UBC-18, but not UBC-3, specifically cooperates with the ARI1s in germline development. These findings provide new insights into the functions of RING-between-RING proteins and Ariadne E3s during development. |
format | Online Article Text |
id | pubmed-6288150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62881502018-12-19 Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans Poush, Julian A. Blouin, Nicolas A. Di Bona, Kristin R. Lažetić, Vladimir Fay, David S. Sci Rep Article RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within the Caenorhabditis clade in recent history, a characteristic shared by some genes involved in germline development. We then examined the effects of deleting all ARI1 family members in the nematode Caenorhabditis elegans, which to our knowledge represents the first complete knockout of ARI1 function in a metazoan. Hermaphrodites that lacked or had strongly reduced ARI1 activity had low fecundity and were partially defective in initiation of oocyte differentiation. We provide evidence that the C. elegans ARI1s likely function downstream or in parallel to FBF-1 and FBF-2, two closely related RNA-binding proteins that are required for the switch from spermatogenesis to oogenesis during late larval development. Previous studies have shown that the E2 enzymes UBC-18/UBCH7 and UBC-3/CDC34 can functionally collaborate with ARI1 family members. Our data indicated that UBC-18, but not UBC-3, specifically cooperates with the ARI1s in germline development. These findings provide new insights into the functions of RING-between-RING proteins and Ariadne E3s during development. Nature Publishing Group UK 2018-12-10 /pmc/articles/PMC6288150/ /pubmed/30531803 http://dx.doi.org/10.1038/s41598-018-35691-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Poush, Julian A. Blouin, Nicolas A. Di Bona, Kristin R. Lažetić, Vladimir Fay, David S. Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title | Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title_full | Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title_fullStr | Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title_full_unstemmed | Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title_short | Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans |
title_sort | regulation of germ cell development by ari1 family ubiquitin ligases in c. elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288150/ https://www.ncbi.nlm.nih.gov/pubmed/30531803 http://dx.doi.org/10.1038/s41598-018-35691-y |
work_keys_str_mv | AT poushjuliana regulationofgermcelldevelopmentbyari1familyubiquitinligasesincelegans AT blouinnicolasa regulationofgermcelldevelopmentbyari1familyubiquitinligasesincelegans AT dibonakristinr regulationofgermcelldevelopmentbyari1familyubiquitinligasesincelegans AT lazeticvladimir regulationofgermcelldevelopmentbyari1familyubiquitinligasesincelegans AT faydavids regulationofgermcelldevelopmentbyari1familyubiquitinligasesincelegans |