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gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1

Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mec...

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Autores principales: Guo, Lijin, Huang, Weiling, Chen, Biao, Jebessa Bekele, Endashaw, Chen, Xiaolan, Cai, Bolin, Nie, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288258/
https://www.ncbi.nlm.nih.gov/pubmed/30564268
http://dx.doi.org/10.3389/fgene.2018.00577
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author Guo, Lijin
Huang, Weiling
Chen, Biao
Jebessa Bekele, Endashaw
Chen, Xiaolan
Cai, Bolin
Nie, Qinghua
author_facet Guo, Lijin
Huang, Weiling
Chen, Biao
Jebessa Bekele, Endashaw
Chen, Xiaolan
Cai, Bolin
Nie, Qinghua
author_sort Guo, Lijin
collection PubMed
description Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1.
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spelling pubmed-62882582018-12-18 gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 Guo, Lijin Huang, Weiling Chen, Biao Jebessa Bekele, Endashaw Chen, Xiaolan Cai, Bolin Nie, Qinghua Front Genet Genetics Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1. Frontiers Media S.A. 2018-12-04 /pmc/articles/PMC6288258/ /pubmed/30564268 http://dx.doi.org/10.3389/fgene.2018.00577 Text en Copyright © 2018 Guo, Huang, Chen, Jebessa Bekele, Chen, Cai and Nie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Guo, Lijin
Huang, Weiling
Chen, Biao
Jebessa Bekele, Endashaw
Chen, Xiaolan
Cai, Bolin
Nie, Qinghua
gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title_full gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title_fullStr gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title_full_unstemmed gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title_short gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
title_sort gga-mir-133a-3p regulates myoblasts proliferation and differentiation by targeting prrx1
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288258/
https://www.ncbi.nlm.nih.gov/pubmed/30564268
http://dx.doi.org/10.3389/fgene.2018.00577
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