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gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1
Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mec...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288258/ https://www.ncbi.nlm.nih.gov/pubmed/30564268 http://dx.doi.org/10.3389/fgene.2018.00577 |
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author | Guo, Lijin Huang, Weiling Chen, Biao Jebessa Bekele, Endashaw Chen, Xiaolan Cai, Bolin Nie, Qinghua |
author_facet | Guo, Lijin Huang, Weiling Chen, Biao Jebessa Bekele, Endashaw Chen, Xiaolan Cai, Bolin Nie, Qinghua |
author_sort | Guo, Lijin |
collection | PubMed |
description | Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1. |
format | Online Article Text |
id | pubmed-6288258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62882582018-12-18 gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 Guo, Lijin Huang, Weiling Chen, Biao Jebessa Bekele, Endashaw Chen, Xiaolan Cai, Bolin Nie, Qinghua Front Genet Genetics Non-coding RNAs play a regulatory role in the growth and development of skeletal muscle. Our previous study suggested that gga-mir-133a-3p was a potential candidate for regulating myoblast proliferation and differentiation in skeletal muscle. The purpose of our study was to reveal the regulatory mechanism of gga-mir-133a-3p in the proliferation and differentiation of chicken myoblasts. Through the detection of cell proliferation activity, cell cycle progression and EdU, we found that gga-mir-133a-3p can significantly inhibit the proliferation of myoblasts. In the process of myogenic differentiation, gga-mir-133a-3p is up-regulated, while gga-mir-133a-3p can significantly promote the up-regulation of differentiation-related muscle-derived factors, indicating that gga-mir-133a-3p can promote the differentiation of myoblasts. Validation at the transcriptional level and protein level proved that gga-mir-133a-3p can inhibit the expression of PRRX1, and the dual-luciferase assay also showed their direct targeting relationship. Correspondingly, PRRX1 can significantly promote myoblast proliferation and inhibit myoblast differentiation. In our study, we confirmed that gga-mir-133a-3p participates in the regulation of proliferation and differentiation of myoblasts by targeting PRRX1. Frontiers Media S.A. 2018-12-04 /pmc/articles/PMC6288258/ /pubmed/30564268 http://dx.doi.org/10.3389/fgene.2018.00577 Text en Copyright © 2018 Guo, Huang, Chen, Jebessa Bekele, Chen, Cai and Nie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Guo, Lijin Huang, Weiling Chen, Biao Jebessa Bekele, Endashaw Chen, Xiaolan Cai, Bolin Nie, Qinghua gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title | gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title_full | gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title_fullStr | gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title_full_unstemmed | gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title_short | gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1 |
title_sort | gga-mir-133a-3p regulates myoblasts proliferation and differentiation by targeting prrx1 |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288258/ https://www.ncbi.nlm.nih.gov/pubmed/30564268 http://dx.doi.org/10.3389/fgene.2018.00577 |
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