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Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos

We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescen...

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Detalles Bibliográficos
Autores principales: Murko, Christina, Vieceli, Felipe Monteleone, Bronner, Marianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288396/
https://www.ncbi.nlm.nih.gov/pubmed/30761336
http://dx.doi.org/10.1016/j.dib.2018.11.109
Descripción
Sumario:We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.