Cargando…

Improvement of solubility and refolding of an anti-human epidermal growth factor receptor 2 single-chain antibody fragment inclusion bodies

Single chain variable fragment antibodies (scFvs) have attracted many attentions due to their small size, faster bio-distribution and better penetration in to the target tissues, and ease of expression in Escherichia coli. Although, scFv expression in E. coli usually leads to formation of inclusion...

Descripción completa

Detalles Bibliográficos
Autores principales:	Salehinia, Javad, Sadeghi, Hamid Mir Mohammad, Abedi, Daryoush, Akbari, Vajihe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Medknow Publications & Media Pvt Ltd 2018
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288993/ https://www.ncbi.nlm.nih.gov/pubmed/30607154 http://dx.doi.org/10.4103/1735-5362.245968

Ejemplares similares

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies
por: Esmaili, Iman, et al.
Publicado: (2018)

Optimization of the expression of phaC2 encoding poly (3-hydroxyalkanoate) synthase from Pseudomonas aeruginosa PTCC1310 in Fad B deleted Escherichia coli
por: Abedi, Daryoush, et al.
Publicado: (2016)

Optimization of the Expression of Genes Encoding Poly (3-hydroxyalkanoate) Synthase from Pseudomonas aeruginosa PTCC 1310 in Escherichia coli
por: Abedi, Daryoush, et al.
Publicado: (2012)

Chemical Assistance in Refolding of Bacterial Inclusion Bodies
por: Alibolandi, Mona, et al.
Publicado: (2011)

Enhancement of Solubility, Purification, and Inclusion Body Refolding of Active Human Mitochondrial Aldehyde Dehydrogenase 2
por: Zhao, Tingting, et al.
Publicado: (2021)

Optimization of a single-chain antibody fragment overexpression in Escherichia coli using response surface methodology
por: Akbari, V., et al.
Publicado: (2015)

Release Studies on Ciprofloxacin Loaded Non-ionic Surfactant Vesicles
por: Akbari, Vajihe, et al.
Publicado: (2015)

Surface plasmon resonance (SPR) based binding studies of refolded single chain antibody fragments
por: Singh, Pranveer
Publicado: (2018)

Co-Expression of Chaperones for Improvement of Soluble Expression and Purification of An Anti-HER2 scFv in Escherichia Coli
por: Estabragh, Amir Mirzapour, et al.
Publicado: (2022)

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer
por: Fazeli, Behnaz, et al.
Publicado: (2019)

Highly Efficient Production of Soluble Proteins from Insoluble Inclusion Bodies by a Two-Step-Denaturing and Refolding Method
por: Yang, Zhong, et al.
Publicado: (2011)

Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2
por: Nasrabadi, Davood, et al.
Publicado: (2018)

Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding
por: Piao, Da-Chuan, et al.
Publicado: (2016)

Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins
por: Vallejo, Luis Felipe, et al.
Publicado: (2004)

Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies
por: Yamaguchi, Hiroshi, et al.
Publicado: (2014)

Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies
por: Qu, Jian-Bo, et al.
Publicado: (2020)

Retraction of “Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies”
por: Qu, Jian-Bo, et al.
Publicado: (2021)

Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method
por: Singhvi, Priyank, et al.
Publicado: (2021)

Refolded scFv Antibody Fragment against Myoglobin Shows Rapid Reaction Kinetics
por: Song, Hyung-Nam, et al.
Publicado: (2014)

Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity
por: Maksum, Iman Permana, et al.
Publicado: (2022)

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology
por: Zare, Hamze, et al.
Publicado: (2019)

Rational design of a new mutant of tobacco etch virus protease in order to increase the in vitro solubility
por: Mohammadian, Hossein, et al.
Publicado: (2020)

Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein
por: Upadhyay, Arun K., et al.
Publicado: (2014)

Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies
por: Heim, Bastian, et al.
Publicado: (2021)

Self-reporting and refoldable profluorescent single-chain nanoparticles
por: Fischer, Tobias S., et al.
Publicado: (2018)

Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts
por: Ban, Bhupal, et al.
Publicado: (2020)

Optimization of Buffer Additives for Efficient Recovery of hGM-CSF from Inclusion Bodies Using Response Surface Methodology
por: Ahmadian, Mina, et al.
Publicado: (2020)

The REFOLD database: a tool for the optimization of protein expression and refolding
por: Chow, Michelle K. M., et al.
Publicado: (2006)

MB109 as bioactive human bone morphogenetic protein-9 refolded and purified from E. coli inclusion bodies
por: Kuo, Mario Meng-Chiang, et al.
Publicado: (2014)

A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form
por: Qi, Xingmei, et al.
Publicado: (2015)

Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus
por: Rosa da Silva, Cleide Mara, et al.
Publicado: (2018)

Prokaryotic expression, refolding, and purification of fragment 450–650 of the spike protein of SARS-coronavirus
por: Zhao, Jin-Cun, et al.
Publicado: (2005)

Effect of Twine-arginine Translocation-signaling Fusion System and Chaperones Co-expression on Secretory Expression of Somatropin
por: Bagherinejad, Mohammad Reza, et al.
Publicado: (2018)

Accelerated simulation of unfolding and refolding of a large single chain globular protein
por: Seddon, Gavin M., et al.
Publicado: (2012)

Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding
por: Chura-Chambi, Rosa Maria, et al.
Publicado: (2022)

Bioassay-guided isolation of glycolipids from the seaweed Gracilaria corticata
por: Akbari, Vajihe, et al.
Publicado: (2020)

Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes
por: Wang, Nan, et al.
Publicado: (2016)

Twin arginine translocation system in secretory expression of recombinant human growth hormone
por: Bagherinejad, Mohammad Reza, et al.
Publicado: (2016)

Production of a Single‐chain Variable Fragment Antibody Recognizing Type III Mutant Epidermal Growth Factor Receptor
por: Nakayashiki, Norihisa, et al.
Publicado: (2000)

A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments
por: Wang, Yang, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_4lliupgthuur1lkrqgehst8m5a