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The study of multivalent carbohydrate–protein interactions by bio-layer interferometry

The study of complex multivalent carbohydrate–protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Us...

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Detalles Bibliográficos
Autores principales: Laigre, Eugénie, Goyard, David, Tiertant, Claire, Dejeu, Jérôme, Renaudet, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289105/
https://www.ncbi.nlm.nih.gov/pubmed/30264842
http://dx.doi.org/10.1039/c8ob01664j
Descripción
Sumario:The study of complex multivalent carbohydrate–protein interactions remains highly complicated and sometimes rendered impossible due to aggregation problems. In this study, we demonstrate that bio-layer interferometry is an excellent complementary method to standard techniques such as SPR and ITC. Using tetra- and hexadecavalent GalNAc glycoconjugates and Helix pomatia agglutinin (HPA) as a model lectin, we were able to measure reliable kinetic and thermodynamic parameters of multivalent interactions going from the micro to the nanomolar range.