Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states o...

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Detalles Bibliográficos
Autores principales: Hellwig, Nils, Peetz, Oliver, Ahdash, Zainab, Tascón, Igor, Booth, Paula J., Mikusevic, Vedrana, Diskowski, Marina, Politis, Argyris, Hellmich, Yvonne, Hänelt, Inga, Reading, Eamonn, Morgner, Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289172/
https://www.ncbi.nlm.nih.gov/pubmed/30452022
http://dx.doi.org/10.1039/c8cc06284f
Descripción
Sumario:Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.

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