Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs

Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states o...

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Detalles Bibliográficos
Autores principales: Hellwig, Nils, Peetz, Oliver, Ahdash, Zainab, Tascón, Igor, Booth, Paula J., Mikusevic, Vedrana, Diskowski, Marina, Politis, Argyris, Hellmich, Yvonne, Hänelt, Inga, Reading, Eamonn, Morgner, Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289172/
https://www.ncbi.nlm.nih.gov/pubmed/30452022
http://dx.doi.org/10.1039/c8cc06284f
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author Hellwig, Nils
Peetz, Oliver
Ahdash, Zainab
Tascón, Igor
Booth, Paula J.
Mikusevic, Vedrana
Diskowski, Marina
Politis, Argyris
Hellmich, Yvonne
Hänelt, Inga
Reading, Eamonn
Morgner, Nina
author_facet Hellwig, Nils
Peetz, Oliver
Ahdash, Zainab
Tascón, Igor
Booth, Paula J.
Mikusevic, Vedrana
Diskowski, Marina
Politis, Argyris
Hellmich, Yvonne
Hänelt, Inga
Reading, Eamonn
Morgner, Nina
author_sort Hellwig, Nils
collection PubMed
description Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs.
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spelling pubmed-62891722019-01-09 Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs Hellwig, Nils Peetz, Oliver Ahdash, Zainab Tascón, Igor Booth, Paula J. Mikusevic, Vedrana Diskowski, Marina Politis, Argyris Hellmich, Yvonne Hänelt, Inga Reading, Eamonn Morgner, Nina Chem Commun (Camb) Chemistry Other than more widely used methods, the use of styrene maleic acid allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding. Here we present the combined use of SMALPs and LILBID-MS, allowing determination of oligomeric states of membrane proteins of different functionality directly from the native nanodiscs. Royal Society of Chemistry 2018-12-18 2018-11-19 /pmc/articles/PMC6289172/ /pubmed/30452022 http://dx.doi.org/10.1039/c8cc06284f Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Hellwig, Nils
Peetz, Oliver
Ahdash, Zainab
Tascón, Igor
Booth, Paula J.
Mikusevic, Vedrana
Diskowski, Marina
Politis, Argyris
Hellmich, Yvonne
Hänelt, Inga
Reading, Eamonn
Morgner, Nina
Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title_full Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title_fullStr Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title_full_unstemmed Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title_short Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
title_sort native mass spectrometry goes more native: investigation of membrane protein complexes directly from smalps
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289172/
https://www.ncbi.nlm.nih.gov/pubmed/30452022
http://dx.doi.org/10.1039/c8cc06284f
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