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Cell-Selective Pore Forming Antimicrobial Peptides of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence Mapping, Membrane Disruption, and Atomic-Resolution Structure and Dynamics
[Image: see text] Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain of furin, a serine protease, expressed in all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial peptides we...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289565/ https://www.ncbi.nlm.nih.gov/pubmed/30555984 http://dx.doi.org/10.1021/acsomega.8b01876 |
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author | Sinha, Sheetal Harioudh, Munesh Kumar Dewangan, Rikeshwer P. Ng, Wun Jern Ghosh, Jimut Kanti Bhattacharjya, Surajit |
author_facet | Sinha, Sheetal Harioudh, Munesh Kumar Dewangan, Rikeshwer P. Ng, Wun Jern Ghosh, Jimut Kanti Bhattacharjya, Surajit |
author_sort | Sinha, Sheetal |
collection | PubMed |
description | [Image: see text] Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain of furin, a serine protease, expressed in all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial peptides were mapped by extensive analyses of overlapping peptide fragments of the prodomain of human furin. Two peptides, YR26 and YR23, were active against bacterial cells including MRSA-resistant Staphylococcus aureus and Staphylococcus epidermis 51625. Peptides were largely devoid of hemolytic and cytotoxic activity. Bacterial cell killing occurred as a result of the disruption of the permeability barrier of the lipopolysaccharide (LPS)-outer membrane and fragmentation of LPS into small micelles. Furthermore, antibacterial peptides specifically interacted with the negatively charged lipids causing membrane leakage and fusion. The YR26 peptide in sodium dodecyl sulfate micelles demonstrated a long-helix-turn-short-helix structure exhibiting restricted backbone motions. The cell-selective activity of the furin peptides and their unique mode of action on membranes have a significant potential for the development of therapeutics. |
format | Online Article Text |
id | pubmed-6289565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-62895652018-12-12 Cell-Selective Pore Forming Antimicrobial Peptides of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence Mapping, Membrane Disruption, and Atomic-Resolution Structure and Dynamics Sinha, Sheetal Harioudh, Munesh Kumar Dewangan, Rikeshwer P. Ng, Wun Jern Ghosh, Jimut Kanti Bhattacharjya, Surajit ACS Omega [Image: see text] Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain of furin, a serine protease, expressed in all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial peptides were mapped by extensive analyses of overlapping peptide fragments of the prodomain of human furin. Two peptides, YR26 and YR23, were active against bacterial cells including MRSA-resistant Staphylococcus aureus and Staphylococcus epidermis 51625. Peptides were largely devoid of hemolytic and cytotoxic activity. Bacterial cell killing occurred as a result of the disruption of the permeability barrier of the lipopolysaccharide (LPS)-outer membrane and fragmentation of LPS into small micelles. Furthermore, antibacterial peptides specifically interacted with the negatively charged lipids causing membrane leakage and fusion. The YR26 peptide in sodium dodecyl sulfate micelles demonstrated a long-helix-turn-short-helix structure exhibiting restricted backbone motions. The cell-selective activity of the furin peptides and their unique mode of action on membranes have a significant potential for the development of therapeutics. American Chemical Society 2018-11-01 /pmc/articles/PMC6289565/ /pubmed/30555984 http://dx.doi.org/10.1021/acsomega.8b01876 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Sinha, Sheetal Harioudh, Munesh Kumar Dewangan, Rikeshwer P. Ng, Wun Jern Ghosh, Jimut Kanti Bhattacharjya, Surajit Cell-Selective Pore Forming Antimicrobial Peptides of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence Mapping, Membrane Disruption, and Atomic-Resolution Structure and Dynamics |
title | Cell-Selective Pore Forming Antimicrobial Peptides
of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence
Mapping, Membrane Disruption, and Atomic-Resolution Structure and
Dynamics |
title_full | Cell-Selective Pore Forming Antimicrobial Peptides
of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence
Mapping, Membrane Disruption, and Atomic-Resolution Structure and
Dynamics |
title_fullStr | Cell-Selective Pore Forming Antimicrobial Peptides
of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence
Mapping, Membrane Disruption, and Atomic-Resolution Structure and
Dynamics |
title_full_unstemmed | Cell-Selective Pore Forming Antimicrobial Peptides
of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence
Mapping, Membrane Disruption, and Atomic-Resolution Structure and
Dynamics |
title_short | Cell-Selective Pore Forming Antimicrobial Peptides
of the Prodomain of Human Furin: A Conserved Aromatic/Cationic Sequence
Mapping, Membrane Disruption, and Atomic-Resolution Structure and
Dynamics |
title_sort | cell-selective pore forming antimicrobial peptides
of the prodomain of human furin: a conserved aromatic/cationic sequence
mapping, membrane disruption, and atomic-resolution structure and
dynamics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289565/ https://www.ncbi.nlm.nih.gov/pubmed/30555984 http://dx.doi.org/10.1021/acsomega.8b01876 |
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