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Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease

Introduction of hyperpolarized magnetic resonance in preclinical studies and lately translation to patients provides new detailed in vivo information of metabolic flux in organs. Hyperpolarized magnetic resonance based on (13)C enriched pyruvate is performed without ionizing radiation and allows qua...

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Autores principales: Kjærgaard, Uffe, Laustsen, Christoffer, Nørlinger, Thomas, Tougaard, Rasmus S., Mikkelsen, Emmeli, Qi, Haiyun, Bertelsen, Lotte B., Jessen, Niels, Stødkilde‐Jørgensen, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289910/
https://www.ncbi.nlm.nih.gov/pubmed/30548433
http://dx.doi.org/10.14814/phy2.13943
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author Kjærgaard, Uffe
Laustsen, Christoffer
Nørlinger, Thomas
Tougaard, Rasmus S.
Mikkelsen, Emmeli
Qi, Haiyun
Bertelsen, Lotte B.
Jessen, Niels
Stødkilde‐Jørgensen, Hans
author_facet Kjærgaard, Uffe
Laustsen, Christoffer
Nørlinger, Thomas
Tougaard, Rasmus S.
Mikkelsen, Emmeli
Qi, Haiyun
Bertelsen, Lotte B.
Jessen, Niels
Stødkilde‐Jørgensen, Hans
author_sort Kjærgaard, Uffe
collection PubMed
description Introduction of hyperpolarized magnetic resonance in preclinical studies and lately translation to patients provides new detailed in vivo information of metabolic flux in organs. Hyperpolarized magnetic resonance based on (13)C enriched pyruvate is performed without ionizing radiation and allows quantification of the pyruvate conversion products: alanine, lactate and bicarbonate in real time. Thus, this methodology has a promising potential for in vivo monitoring of energetic alterations in hepatic diseases. Using (13)C pyruvate, we investigated the metabolism in the porcine liver before and after intravenous injection of glucose. The overall mean lactate to pyruvate ratio increased significantly after the injection of glucose whereas the bicarbonate to pyruvate ratio was unaffected, representative of the levels of pyruvate entering the tricarboxylic acid cycle. Similarly, alanine to pyruvate ratio did not change. The increased lactate to pyruvate ratio over time showed an exponential correlation with insulin, glucagon and free fatty acids. Together, these data, obtained by hyperpolarized (13)C magnetic resonance spectroscopy and by blood sampling, indicate a hepatic metabolic shift in glucose utilization following a glucose challenge. Our findings demonstrate the capacity of hyperpolarized (13)C magnetic resonance spectroscopy for quantifying hepatic substrate metabolism in accordance with well‐known physiological processes. When combined with concentration of blood insulin, glucagon and free fatty acids in the blood, the results indicate the potential of hyperpolarized magnetic resonance spectroscopy as a future clinical method for quantification of hepatic substrate metabolism.
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spelling pubmed-62899102018-12-17 Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease Kjærgaard, Uffe Laustsen, Christoffer Nørlinger, Thomas Tougaard, Rasmus S. Mikkelsen, Emmeli Qi, Haiyun Bertelsen, Lotte B. Jessen, Niels Stødkilde‐Jørgensen, Hans Physiol Rep Original Research Introduction of hyperpolarized magnetic resonance in preclinical studies and lately translation to patients provides new detailed in vivo information of metabolic flux in organs. Hyperpolarized magnetic resonance based on (13)C enriched pyruvate is performed without ionizing radiation and allows quantification of the pyruvate conversion products: alanine, lactate and bicarbonate in real time. Thus, this methodology has a promising potential for in vivo monitoring of energetic alterations in hepatic diseases. Using (13)C pyruvate, we investigated the metabolism in the porcine liver before and after intravenous injection of glucose. The overall mean lactate to pyruvate ratio increased significantly after the injection of glucose whereas the bicarbonate to pyruvate ratio was unaffected, representative of the levels of pyruvate entering the tricarboxylic acid cycle. Similarly, alanine to pyruvate ratio did not change. The increased lactate to pyruvate ratio over time showed an exponential correlation with insulin, glucagon and free fatty acids. Together, these data, obtained by hyperpolarized (13)C magnetic resonance spectroscopy and by blood sampling, indicate a hepatic metabolic shift in glucose utilization following a glucose challenge. Our findings demonstrate the capacity of hyperpolarized (13)C magnetic resonance spectroscopy for quantifying hepatic substrate metabolism in accordance with well‐known physiological processes. When combined with concentration of blood insulin, glucagon and free fatty acids in the blood, the results indicate the potential of hyperpolarized magnetic resonance spectroscopy as a future clinical method for quantification of hepatic substrate metabolism. John Wiley and Sons Inc. 2018-12-11 /pmc/articles/PMC6289910/ /pubmed/30548433 http://dx.doi.org/10.14814/phy2.13943 Text en © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Kjærgaard, Uffe
Laustsen, Christoffer
Nørlinger, Thomas
Tougaard, Rasmus S.
Mikkelsen, Emmeli
Qi, Haiyun
Bertelsen, Lotte B.
Jessen, Niels
Stødkilde‐Jørgensen, Hans
Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title_full Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title_fullStr Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title_full_unstemmed Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title_short Hyperpolarized [1‐(13)C] pyruvate as a possible diagnostic tool in liver disease
title_sort hyperpolarized [1‐(13)c] pyruvate as a possible diagnostic tool in liver disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289910/
https://www.ncbi.nlm.nih.gov/pubmed/30548433
http://dx.doi.org/10.14814/phy2.13943
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