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Engineering and modification of microbial chassis for systems and synthetic biology

Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural, veterinary, and food industries. The two primary strategies for constructing a microbial chassi...

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Autores principales: Chi, Haotian, Wang, Xiaoli, Shao, Yue, Qin, Ying, Deng, Zixin, Wang, Lianrong, Chen, Shi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290258/
https://www.ncbi.nlm.nih.gov/pubmed/30560208
http://dx.doi.org/10.1016/j.synbio.2018.12.001
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author Chi, Haotian
Wang, Xiaoli
Shao, Yue
Qin, Ying
Deng, Zixin
Wang, Lianrong
Chen, Shi
author_facet Chi, Haotian
Wang, Xiaoli
Shao, Yue
Qin, Ying
Deng, Zixin
Wang, Lianrong
Chen, Shi
author_sort Chi, Haotian
collection PubMed
description Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural, veterinary, and food industries. The two primary strategies for constructing a microbial chassis are the top-down approach (genome reduction) and the bottom-up approach (genome synthesis). Research programs on this topic have been funded in several countries. The ‘Minimum genome factory’ (MGF) project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use. One of the best examples of the results of this project is E. coli MGF-01, which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain [1]. The ‘cell factory’ project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU (European Union), which tried to comprehensively understand microorganisms used in the application field. One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis, which was summarized as the ‘secretome’ [2]. The GTL (Genomes to Life) program began in 2002 in the United States. In this program, researchers aimed to create artificial cells both in silico and in vitro, such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group [3]. This review provides an update on recent advances in engineering, modification and application of synthetic microbial chassis, with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications.
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spelling pubmed-62902582018-12-17 Engineering and modification of microbial chassis for systems and synthetic biology Chi, Haotian Wang, Xiaoli Shao, Yue Qin, Ying Deng, Zixin Wang, Lianrong Chen, Shi Synth Syst Biotechnol Article Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural, veterinary, and food industries. The two primary strategies for constructing a microbial chassis are the top-down approach (genome reduction) and the bottom-up approach (genome synthesis). Research programs on this topic have been funded in several countries. The ‘Minimum genome factory’ (MGF) project was launched in 2001 in Japan with the goal of constructing microorganisms with smaller genomes for industrial use. One of the best examples of the results of this project is E. coli MGF-01, which has a reduced-genome size and exhibits better growth and higher threonine production characteristics than the parental strain [1]. The ‘cell factory’ project was carried out from 1998 to 2002 in the Fifth Framework Program of the EU (European Union), which tried to comprehensively understand microorganisms used in the application field. One of the outstanding results of this project was the elucidation of proteins secreted by Bacillus subtilis, which was summarized as the ‘secretome’ [2]. The GTL (Genomes to Life) program began in 2002 in the United States. In this program, researchers aimed to create artificial cells both in silico and in vitro, such as the successful design and synthesis of a minimal bacterial genome by John Craig Venter's group [3]. This review provides an update on recent advances in engineering, modification and application of synthetic microbial chassis, with particular emphasis on the value of learning about chassis as a way to better understand life and improve applications. KeAi Publishing 2018-12-11 /pmc/articles/PMC6290258/ /pubmed/30560208 http://dx.doi.org/10.1016/j.synbio.2018.12.001 Text en © 2019 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Chi, Haotian
Wang, Xiaoli
Shao, Yue
Qin, Ying
Deng, Zixin
Wang, Lianrong
Chen, Shi
Engineering and modification of microbial chassis for systems and synthetic biology
title Engineering and modification of microbial chassis for systems and synthetic biology
title_full Engineering and modification of microbial chassis for systems and synthetic biology
title_fullStr Engineering and modification of microbial chassis for systems and synthetic biology
title_full_unstemmed Engineering and modification of microbial chassis for systems and synthetic biology
title_short Engineering and modification of microbial chassis for systems and synthetic biology
title_sort engineering and modification of microbial chassis for systems and synthetic biology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290258/
https://www.ncbi.nlm.nih.gov/pubmed/30560208
http://dx.doi.org/10.1016/j.synbio.2018.12.001
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