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Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells
Genital inflammatory cytokine responses increase HIV risk. Since male partner semen is a complex mixture of immune-modulatory prostaglandins and cytokines, we hypothesized that exposure to semen may influence genital inflammation in women. Here, we investigated cytokine response kinetics of cervical...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290331/ https://www.ncbi.nlm.nih.gov/pubmed/30568652 http://dx.doi.org/10.3389/fimmu.2018.02721 |
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author | Rametse, Cosnet L. Adefuye, Anthonio O. Olivier, Abraham J. Curry, Lyle Gamieldien, Hoyam Burgers, Wendy A. Lewis, David A. Williamson, Anna-Lise Katz, Arieh A. Passmore, Jo-Ann S. |
author_facet | Rametse, Cosnet L. Adefuye, Anthonio O. Olivier, Abraham J. Curry, Lyle Gamieldien, Hoyam Burgers, Wendy A. Lewis, David A. Williamson, Anna-Lise Katz, Arieh A. Passmore, Jo-Ann S. |
author_sort | Rametse, Cosnet L. |
collection | PubMed |
description | Genital inflammatory cytokine responses increase HIV risk. Since male partner semen is a complex mixture of immune-modulatory prostaglandins and cytokines, we hypothesized that exposure to semen may influence genital inflammation in women. Here, we investigated cytokine response kinetics of cervical cells following stimulation with seminal plasma from HIV-negative and HIV-positive men characterized as having low or high concentrations of inflammatory cytokines. Irrespective of the HIV status or semen cytokine profile, in vitro stimulation of cervical cells with seminal plasma resulted in significantly elevated concentrations of secreted IL-6, IL-8, TNF-β, MCP-1, GM-CSF, and VEGF within 8 h of stimulation, which tended to decline by 24 h, although this was only significant for TNF-β. Consistent with this, cervical cells responded to seminal plasma with increases in IL-8 and IL-1β mRNA expression of 10-fold. These findings suggest that the impact of semen on local female genital cytokines is likely transient. Although these findings suggest that the impact of semen on local female genital cytokines may not be sustained long-term, this heightened genital inflammation may have implications for HIV risk in women. |
format | Online Article Text |
id | pubmed-6290331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62903312018-12-19 Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells Rametse, Cosnet L. Adefuye, Anthonio O. Olivier, Abraham J. Curry, Lyle Gamieldien, Hoyam Burgers, Wendy A. Lewis, David A. Williamson, Anna-Lise Katz, Arieh A. Passmore, Jo-Ann S. Front Immunol Immunology Genital inflammatory cytokine responses increase HIV risk. Since male partner semen is a complex mixture of immune-modulatory prostaglandins and cytokines, we hypothesized that exposure to semen may influence genital inflammation in women. Here, we investigated cytokine response kinetics of cervical cells following stimulation with seminal plasma from HIV-negative and HIV-positive men characterized as having low or high concentrations of inflammatory cytokines. Irrespective of the HIV status or semen cytokine profile, in vitro stimulation of cervical cells with seminal plasma resulted in significantly elevated concentrations of secreted IL-6, IL-8, TNF-β, MCP-1, GM-CSF, and VEGF within 8 h of stimulation, which tended to decline by 24 h, although this was only significant for TNF-β. Consistent with this, cervical cells responded to seminal plasma with increases in IL-8 and IL-1β mRNA expression of 10-fold. These findings suggest that the impact of semen on local female genital cytokines is likely transient. Although these findings suggest that the impact of semen on local female genital cytokines may not be sustained long-term, this heightened genital inflammation may have implications for HIV risk in women. Frontiers Media S.A. 2018-12-04 /pmc/articles/PMC6290331/ /pubmed/30568652 http://dx.doi.org/10.3389/fimmu.2018.02721 Text en Copyright © 2018 Rametse, Adefuye, Olivier, Curry, Gamieldien, Burgers, Lewis, Williamson, Katz and Passmore. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Rametse, Cosnet L. Adefuye, Anthonio O. Olivier, Abraham J. Curry, Lyle Gamieldien, Hoyam Burgers, Wendy A. Lewis, David A. Williamson, Anna-Lise Katz, Arieh A. Passmore, Jo-Ann S. Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title | Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title_full | Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title_fullStr | Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title_full_unstemmed | Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title_short | Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells |
title_sort | inflammatory cytokine profiles of semen influence cytokine responses of cervicovaginal epithelial cells |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290331/ https://www.ncbi.nlm.nih.gov/pubmed/30568652 http://dx.doi.org/10.3389/fimmu.2018.02721 |
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