Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions

A new simple scheme for constructing recombinant vectors that does not require any restriction enzyme, ligase, or any other special enzyme treatment has been developed. By using caged primers in PCR, unnatural sticky-ends of any sequence, which are sufficiently long for ligation-independent cloning...

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Detalles Bibliográficos
Autores principales: Kuzuya, Akinori, Tanaka, Keita, Katada, Hitoshi, Komiyama, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290560/
https://www.ncbi.nlm.nih.gov/pubmed/22210171
http://dx.doi.org/10.3390/molecules17010328

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290560/
https://www.ncbi.nlm.nih.gov/pubmed/22210171
http://dx.doi.org/10.3390/molecules17010328

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