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Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

BACKGROUND: Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reagg...

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Detalles Bibliográficos
Autores principales:	Rosa da Silva, Cleide Mara, Chura-Chambi, Rosa Maria, Ramos Pereira, Lennon, Cordeiro, Yraima, de Souza Ferreira, Luís Carlos, Morganti, Ligia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291932/ https://www.ncbi.nlm.nih.gov/pubmed/30541520 http://dx.doi.org/10.1186/s12896-018-0486-2

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