Cargando…
Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot
BACKGROUND: Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify the possible source of infection, the geographical origin of the parasite and to a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291991/ https://www.ncbi.nlm.nih.gov/pubmed/30541617 http://dx.doi.org/10.1186/s13071-018-3244-3 |
_version_ | 1783380322966044672 |
---|---|
author | Gómez-Morales, Maria Angeles Ludovisi, Alessandra Amati, Marco Cherchi, Simona Tonanzi, Daniele Pozio, Edoardo |
author_facet | Gómez-Morales, Maria Angeles Ludovisi, Alessandra Amati, Marco Cherchi, Simona Tonanzi, Daniele Pozio, Edoardo |
author_sort | Gómez-Morales, Maria Angeles |
collection | PubMed |
description | BACKGROUND: Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify the possible source of infection, the geographical origin of the parasite and to assess risk of infection for domestic pigs and humans. Specific identification of the etiological agent is not always feasible using direct methods since the source of infection can be untraceable. The aim of this study was to develop a diagnostic tool to infer the causative Trichinella species using western blot patterns of sera derived from infected animal and human hosts. METHODS: Sera from mice experimentally infected with Trichinella spiralis, Trichinella britovi, Trichinella pseudospiralis and Trichinella papuae were tested by western blot using homologous and heterologous crude worm extracts (CWE) and a highly sensitive detection system based on chemiluminescence. In addition, sera from pigs experimentally infected with T. spiralis, T. britovi and T. pseudospiralis and from patients with confirmed T. spiralis, T. britovi and T. pseudospiralis infections, were also included. RESULTS: Sera from mice infected with one Trichinella species reacted with CWE proteins from all four investigated species. Likewise, sera derived from pigs and humans infected with one Trichinella species reacted with CWE proteins from all the three investigated species. Using T. spiralis CWE, sera from T. pseudospiralis-infected hosts yielded a characteristic pattern of reactivity using Wb, which differed to that produced by T. spiralis/T. britovi- or T. papuae-infected host sera. CONCLUSIONS: The present study suggests that western blot using T. spiralis CWE may be a useful tool to distinguish Trichinella infections caused by T. pseudospiralis from those caused by T. spiralis or T. britovi. This method may support epidemiological investigations, particularly when the source of infection is not traceable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3244-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6291991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62919912018-12-17 Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot Gómez-Morales, Maria Angeles Ludovisi, Alessandra Amati, Marco Cherchi, Simona Tonanzi, Daniele Pozio, Edoardo Parasit Vectors Research BACKGROUND: Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify the possible source of infection, the geographical origin of the parasite and to assess risk of infection for domestic pigs and humans. Specific identification of the etiological agent is not always feasible using direct methods since the source of infection can be untraceable. The aim of this study was to develop a diagnostic tool to infer the causative Trichinella species using western blot patterns of sera derived from infected animal and human hosts. METHODS: Sera from mice experimentally infected with Trichinella spiralis, Trichinella britovi, Trichinella pseudospiralis and Trichinella papuae were tested by western blot using homologous and heterologous crude worm extracts (CWE) and a highly sensitive detection system based on chemiluminescence. In addition, sera from pigs experimentally infected with T. spiralis, T. britovi and T. pseudospiralis and from patients with confirmed T. spiralis, T. britovi and T. pseudospiralis infections, were also included. RESULTS: Sera from mice infected with one Trichinella species reacted with CWE proteins from all four investigated species. Likewise, sera derived from pigs and humans infected with one Trichinella species reacted with CWE proteins from all the three investigated species. Using T. spiralis CWE, sera from T. pseudospiralis-infected hosts yielded a characteristic pattern of reactivity using Wb, which differed to that produced by T. spiralis/T. britovi- or T. papuae-infected host sera. CONCLUSIONS: The present study suggests that western blot using T. spiralis CWE may be a useful tool to distinguish Trichinella infections caused by T. pseudospiralis from those caused by T. spiralis or T. britovi. This method may support epidemiological investigations, particularly when the source of infection is not traceable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3244-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-12 /pmc/articles/PMC6291991/ /pubmed/30541617 http://dx.doi.org/10.1186/s13071-018-3244-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Gómez-Morales, Maria Angeles Ludovisi, Alessandra Amati, Marco Cherchi, Simona Tonanzi, Daniele Pozio, Edoardo Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title | Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title_full | Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title_fullStr | Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title_full_unstemmed | Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title_short | Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot |
title_sort | differentiation of trichinella species (trichinella spiralis/trichinella britovi versus trichinella pseudospiralis) using western blot |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291991/ https://www.ncbi.nlm.nih.gov/pubmed/30541617 http://dx.doi.org/10.1186/s13071-018-3244-3 |
work_keys_str_mv | AT gomezmoralesmariaangeles differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot AT ludovisialessandra differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot AT amatimarco differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot AT cherchisimona differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot AT tonanzidaniele differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot AT pozioedoardo differentiationoftrichinellaspeciestrichinellaspiralistrichinellabritoviversustrichinellapseudospiralisusingwesternblot |