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Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients
BACKGROUND: Demineralized dentin matrix (DDM) is commonly used as a bone-graft substitute. This study measured and compared human hepatitis B viruses (HBV) DNA in fresh dentin to that of dentin processed into DDM extracted during dental treatment from HBV-infected patients. The hypothesis was that t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292124/ https://www.ncbi.nlm.nih.gov/pubmed/30541555 http://dx.doi.org/10.1186/s12967-018-1719-9 |
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author | Um, Inwoong Choi, Sungweon Kim, Youngkyun Pang, Kangmi Lee, Jongho Lee, Minsun Kim, Bongju |
author_facet | Um, Inwoong Choi, Sungweon Kim, Youngkyun Pang, Kangmi Lee, Jongho Lee, Minsun Kim, Bongju |
author_sort | Um, Inwoong |
collection | PubMed |
description | BACKGROUND: Demineralized dentin matrix (DDM) is commonly used as a bone-graft substitute. This study measured and compared human hepatitis B viruses (HBV) DNA in fresh dentin to that of dentin processed into DDM extracted during dental treatment from HBV-infected patients. The hypothesis was that the processing procedure for DDM would inactivate or eliminate HBV in the dentin matrix obtained from infected patients. METHODS: Dentin from eighteen HBV-infected patients was collected and each dentin specimen was divided into two fragments. One fragment was used before processing as fresh dentin (control group) and the other was processed into DDM (experimental group). DNA was extracted and purified from each fresh and processed dentin specimen and the HBV DNA copy number quantitated by real time polymerase chain reaction. The HBV DNA copy number in the fresh dentin specimens were compared relative to serologic test results. The second parameter was to evaluate the effectiveness of the processing procedure (defatting, demineralization, freeze-drying, and sterilization) to inactivate or eliminate HBV by comparing the DNA copy number in the processed DDM with that in the matched fresh dentin specimens. All results were analyzed using Mann–Whitney U test to compare numerical measurements between groups and differences were considered statistically significant at P-values less than 0.05. RESULTS: The presence of HBV DNA was detected in 55.56% (10/18) of the fresh dentin specimens. For the ten HBV DNA-positive fresh dentin specimens, HBV DNA was detected in two (20%) of the matched processed dentin specimens. The copy number of HBV DNA in the two positive processed dentin specimens was 1.79 and 4.03, which were statistically lower than that of the fresh dentin specimens (P = 0.0167). CONCLUSIONS: The results from this study suggested that fresh dentin may be a carrier of HBV and that the procedure used to generate DDM extensively reduced the levels of HBV DNA. Further studies are needed to evaluate the infectivity of HBV in processed dentin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-018-1719-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6292124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62921242018-12-17 Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients Um, Inwoong Choi, Sungweon Kim, Youngkyun Pang, Kangmi Lee, Jongho Lee, Minsun Kim, Bongju J Transl Med Research BACKGROUND: Demineralized dentin matrix (DDM) is commonly used as a bone-graft substitute. This study measured and compared human hepatitis B viruses (HBV) DNA in fresh dentin to that of dentin processed into DDM extracted during dental treatment from HBV-infected patients. The hypothesis was that the processing procedure for DDM would inactivate or eliminate HBV in the dentin matrix obtained from infected patients. METHODS: Dentin from eighteen HBV-infected patients was collected and each dentin specimen was divided into two fragments. One fragment was used before processing as fresh dentin (control group) and the other was processed into DDM (experimental group). DNA was extracted and purified from each fresh and processed dentin specimen and the HBV DNA copy number quantitated by real time polymerase chain reaction. The HBV DNA copy number in the fresh dentin specimens were compared relative to serologic test results. The second parameter was to evaluate the effectiveness of the processing procedure (defatting, demineralization, freeze-drying, and sterilization) to inactivate or eliminate HBV by comparing the DNA copy number in the processed DDM with that in the matched fresh dentin specimens. All results were analyzed using Mann–Whitney U test to compare numerical measurements between groups and differences were considered statistically significant at P-values less than 0.05. RESULTS: The presence of HBV DNA was detected in 55.56% (10/18) of the fresh dentin specimens. For the ten HBV DNA-positive fresh dentin specimens, HBV DNA was detected in two (20%) of the matched processed dentin specimens. The copy number of HBV DNA in the two positive processed dentin specimens was 1.79 and 4.03, which were statistically lower than that of the fresh dentin specimens (P = 0.0167). CONCLUSIONS: The results from this study suggested that fresh dentin may be a carrier of HBV and that the procedure used to generate DDM extensively reduced the levels of HBV DNA. Further studies are needed to evaluate the infectivity of HBV in processed dentin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-018-1719-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-12 /pmc/articles/PMC6292124/ /pubmed/30541555 http://dx.doi.org/10.1186/s12967-018-1719-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Um, Inwoong Choi, Sungweon Kim, Youngkyun Pang, Kangmi Lee, Jongho Lee, Minsun Kim, Bongju Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title | Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title_full | Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title_fullStr | Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title_full_unstemmed | Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title_short | Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients |
title_sort | measurement of hepatitis b virus dna in fresh versus processed dentin from chronically infected patients |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292124/ https://www.ncbi.nlm.nih.gov/pubmed/30541555 http://dx.doi.org/10.1186/s12967-018-1719-9 |
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