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Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells
Foetal calf serum (FCS) is a common supplement of cell culture medium and a known source of contaminating extracellular vesicles (EV) containing RNA. Because of a high degree of sequence similarity among homologous non-coding RNAs of mammalian species, residual FCS-RNA in culture medium may interfer...
Autores principales: | Driedonks, Tom A. P., Nijen Twilhaar, Maarten K., Nolte-‘t Hoen, Esther N. M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292350/ https://www.ncbi.nlm.nih.gov/pubmed/30559953 http://dx.doi.org/10.1080/20013078.2018.1552059 |
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