Cargando…
Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices
In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expressio...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293036/ https://www.ncbi.nlm.nih.gov/pubmed/30582064 http://dx.doi.org/10.1016/j.ibror.2018.11.006 |
_version_ | 1783380483462135808 |
---|---|
author | Ueno, Hiroshi Suemitsu, Shunsuke Murakami, Shinji Kitamura, Naoya Wani, Kenta Matsumoto, Yosuke Okamoto, Motoi Ishihara, Takeshi |
author_facet | Ueno, Hiroshi Suemitsu, Shunsuke Murakami, Shinji Kitamura, Naoya Wani, Kenta Matsumoto, Yosuke Okamoto, Motoi Ishihara, Takeshi |
author_sort | Ueno, Hiroshi |
collection | PubMed |
description | In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expression level of granular ECM molecules (diffuse ECM) present in the mature CNS remain unclear. Diffuse ECM molecules in the CNS share the components of PNNs and are likely functional. As cortical functions are greatly region-dependent, we hypothesized that ECM molecules would differ in distribution, expression level, and components in a region- and layer-dependent manner. We examined the layer-specific expression of several chondroitin sulfate proteoglycans (aggrecan, neurocan, and brevican), tenascin-R, Wisteria floribunda agglutinin (WFA)-positive molecules, hyaluronic acid, and link protein in the somatosensory and piriform cortices of mature mice. Furthermore, we investigated expression changes in WFA-positive molecules due to aging. In the somatosensory cortex, PNN density was particularly high at layer 4 (L4), but not all diffuse ECM molecules were highly expressed at L4 compared to the other layers. There was almost no change in tenascin-R and hyaluronic acid in any somatosensory-cortex layer. Neurocan showed high expression in L1 of the somatosensory cortex. In the piriform cortex, many ECM molecules showed higher expression in L1 than in the other layers. However, hyaluronic acid showed high expression in deep layers. Here, we clarified that ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. Region-specific expression of ECM molecules is possibly related to functions such as region-specific plasticity and vulnerability. |
format | Online Article Text |
id | pubmed-6293036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-62930362018-12-21 Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices Ueno, Hiroshi Suemitsu, Shunsuke Murakami, Shinji Kitamura, Naoya Wani, Kenta Matsumoto, Yosuke Okamoto, Motoi Ishihara, Takeshi IBRO Rep Article In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expression level of granular ECM molecules (diffuse ECM) present in the mature CNS remain unclear. Diffuse ECM molecules in the CNS share the components of PNNs and are likely functional. As cortical functions are greatly region-dependent, we hypothesized that ECM molecules would differ in distribution, expression level, and components in a region- and layer-dependent manner. We examined the layer-specific expression of several chondroitin sulfate proteoglycans (aggrecan, neurocan, and brevican), tenascin-R, Wisteria floribunda agglutinin (WFA)-positive molecules, hyaluronic acid, and link protein in the somatosensory and piriform cortices of mature mice. Furthermore, we investigated expression changes in WFA-positive molecules due to aging. In the somatosensory cortex, PNN density was particularly high at layer 4 (L4), but not all diffuse ECM molecules were highly expressed at L4 compared to the other layers. There was almost no change in tenascin-R and hyaluronic acid in any somatosensory-cortex layer. Neurocan showed high expression in L1 of the somatosensory cortex. In the piriform cortex, many ECM molecules showed higher expression in L1 than in the other layers. However, hyaluronic acid showed high expression in deep layers. Here, we clarified that ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. Region-specific expression of ECM molecules is possibly related to functions such as region-specific plasticity and vulnerability. Elsevier 2018-11-28 /pmc/articles/PMC6293036/ /pubmed/30582064 http://dx.doi.org/10.1016/j.ibror.2018.11.006 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Ueno, Hiroshi Suemitsu, Shunsuke Murakami, Shinji Kitamura, Naoya Wani, Kenta Matsumoto, Yosuke Okamoto, Motoi Ishihara, Takeshi Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title | Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title_full | Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title_fullStr | Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title_full_unstemmed | Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title_short | Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
title_sort | layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293036/ https://www.ncbi.nlm.nih.gov/pubmed/30582064 http://dx.doi.org/10.1016/j.ibror.2018.11.006 |
work_keys_str_mv | AT uenohiroshi layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT suemitsushunsuke layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT murakamishinji layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT kitamuranaoya layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT wanikenta layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT matsumotoyosuke layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT okamotomotoi layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices AT ishiharatakeshi layerspecificexpressionofextracellularmatrixmoleculesinthemousesomatosensoryandpiriformcortices |