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Diversity of Rickettsia species in border regions of northwestern China

BACKGROUND: Rickettsia species belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least ten SFG Rickettsia species are known to occur in China. However, the distribution of rickettsiae in ticks and fleas in the border region of northwestern China...

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Autores principales: Song, Shengnan, Chen, Chuangfu, Yang, Meihua, Zhao, Shanshan, Wang, Baoju, Hornok, Sándor, Makhatov, Bolatkhan, Rizabek, Kadyken, Wang, Yuanzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293579/
https://www.ncbi.nlm.nih.gov/pubmed/30545379
http://dx.doi.org/10.1186/s13071-018-3233-6
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author Song, Shengnan
Chen, Chuangfu
Yang, Meihua
Zhao, Shanshan
Wang, Baoju
Hornok, Sándor
Makhatov, Bolatkhan
Rizabek, Kadyken
Wang, Yuanzhi
author_facet Song, Shengnan
Chen, Chuangfu
Yang, Meihua
Zhao, Shanshan
Wang, Baoju
Hornok, Sándor
Makhatov, Bolatkhan
Rizabek, Kadyken
Wang, Yuanzhi
author_sort Song, Shengnan
collection PubMed
description BACKGROUND: Rickettsia species belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least ten SFG Rickettsia species are known to occur in China. However, the distribution of rickettsiae in ticks and fleas in the border region of northwestern China have not been systematically studied to date. RESULTS: A total of 982 ticks (Rhipicephalus turanicus, Dermacentor marginatus, D. nuttalli and Haemaphysalis punctata) and 5052 fleas (18 flea species from 14 species of wild mammals) were collected in ten and five counties, respectively, of Xinjiang Uygur Autonomous Region (northwestern China). Tick and flea species were identified according to morphological and molecular characteristics. Seven sets of primers for amplifying the 17-kDa antigen gene (17-kDa), citrate synthase gene (gltA), 16S rRNA gene (rrs), outer membrane protein A and B genes (ompA, ompB), surface cell antigen 1 gene (sca1) and PS120-protein encoding gene (gene D) were used to identify the species of rickettsiae. Nine Rickettsia species have been detected, seven of them in ticks: R. aeschlimannii, R. conorii, R. raoultii, Rickettsia sibirica, R. slovaca, R. massiliae and “Candidatus R. barbariae”. In addition, R. bellii and two genotypes of a rickettsia endosymbiont (phylogenetically in an ancestral position to R. bellii) have been detected from flea pools. CONCLUSIONS: This study provides molecular evidence for the occurrence of several SFG rickettsiae in Rhipicephalus turanicus, Dermacentor nuttalli and D. marginatus. Furthermore, R. bellii and two ancestral rickettsia endosymbionts are present in fleas infesting wild rodents in the border regions of northwestern China. These data extend our knowledge on the diversity of rickettsiae in Central Asia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3233-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-62935792018-12-18 Diversity of Rickettsia species in border regions of northwestern China Song, Shengnan Chen, Chuangfu Yang, Meihua Zhao, Shanshan Wang, Baoju Hornok, Sándor Makhatov, Bolatkhan Rizabek, Kadyken Wang, Yuanzhi Parasit Vectors Short Report BACKGROUND: Rickettsia species belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least ten SFG Rickettsia species are known to occur in China. However, the distribution of rickettsiae in ticks and fleas in the border region of northwestern China have not been systematically studied to date. RESULTS: A total of 982 ticks (Rhipicephalus turanicus, Dermacentor marginatus, D. nuttalli and Haemaphysalis punctata) and 5052 fleas (18 flea species from 14 species of wild mammals) were collected in ten and five counties, respectively, of Xinjiang Uygur Autonomous Region (northwestern China). Tick and flea species were identified according to morphological and molecular characteristics. Seven sets of primers for amplifying the 17-kDa antigen gene (17-kDa), citrate synthase gene (gltA), 16S rRNA gene (rrs), outer membrane protein A and B genes (ompA, ompB), surface cell antigen 1 gene (sca1) and PS120-protein encoding gene (gene D) were used to identify the species of rickettsiae. Nine Rickettsia species have been detected, seven of them in ticks: R. aeschlimannii, R. conorii, R. raoultii, Rickettsia sibirica, R. slovaca, R. massiliae and “Candidatus R. barbariae”. In addition, R. bellii and two genotypes of a rickettsia endosymbiont (phylogenetically in an ancestral position to R. bellii) have been detected from flea pools. CONCLUSIONS: This study provides molecular evidence for the occurrence of several SFG rickettsiae in Rhipicephalus turanicus, Dermacentor nuttalli and D. marginatus. Furthermore, R. bellii and two ancestral rickettsia endosymbionts are present in fleas infesting wild rodents in the border regions of northwestern China. These data extend our knowledge on the diversity of rickettsiae in Central Asia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3233-6) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-13 /pmc/articles/PMC6293579/ /pubmed/30545379 http://dx.doi.org/10.1186/s13071-018-3233-6 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Song, Shengnan
Chen, Chuangfu
Yang, Meihua
Zhao, Shanshan
Wang, Baoju
Hornok, Sándor
Makhatov, Bolatkhan
Rizabek, Kadyken
Wang, Yuanzhi
Diversity of Rickettsia species in border regions of northwestern China
title Diversity of Rickettsia species in border regions of northwestern China
title_full Diversity of Rickettsia species in border regions of northwestern China
title_fullStr Diversity of Rickettsia species in border regions of northwestern China
title_full_unstemmed Diversity of Rickettsia species in border regions of northwestern China
title_short Diversity of Rickettsia species in border regions of northwestern China
title_sort diversity of rickettsia species in border regions of northwestern china
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293579/
https://www.ncbi.nlm.nih.gov/pubmed/30545379
http://dx.doi.org/10.1186/s13071-018-3233-6
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