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Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model

BACKGROUND: The molecular mechanisms underlying stress-influenced immune function of chicken (Gallus Gallus) are not clear. The stress models can be established effectively by feeding chickens corticosterone (CORT) hormone. The bursa of Fabricius is a unique central immune organ of birds. RNA-Seq te...

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Autores principales: Zhang, Yanhua, Zhou, Yanting, Sun, Guirong, Li, Kui, Li, Zhuanjian, Su, Aru, Liu, Xiaojun, Li, Guoxi, Jiang, Ruirui, Han, Ruili, Tian, Yadong, Kang, Xiangtao, Yan, Fengbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293626/
https://www.ncbi.nlm.nih.gov/pubmed/30545299
http://dx.doi.org/10.1186/s12864-018-5333-2
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author Zhang, Yanhua
Zhou, Yanting
Sun, Guirong
Li, Kui
Li, Zhuanjian
Su, Aru
Liu, Xiaojun
Li, Guoxi
Jiang, Ruirui
Han, Ruili
Tian, Yadong
Kang, Xiangtao
Yan, Fengbin
author_facet Zhang, Yanhua
Zhou, Yanting
Sun, Guirong
Li, Kui
Li, Zhuanjian
Su, Aru
Liu, Xiaojun
Li, Guoxi
Jiang, Ruirui
Han, Ruili
Tian, Yadong
Kang, Xiangtao
Yan, Fengbin
author_sort Zhang, Yanhua
collection PubMed
description BACKGROUND: The molecular mechanisms underlying stress-influenced immune function of chicken (Gallus Gallus) are not clear. The stress models can be established effectively by feeding chickens corticosterone (CORT) hormone. The bursa of Fabricius is a unique central immune organ of birds. RNA-Seq technology was used to investigate differences in the expression profiles of immune-related genes and associated pathways in the bursa of Fabricius to clarify molecular mechanisms. The aim of this study was to broaden the understanding of the stress-influenced immune function in chickens. RESULTS: Differentially expressed genes (DEGs) in the bursa of Fabricius between experimental group (basal diet with added CORT 30 mg/kg; C_B group) and control group (basal diet; B_B group) were identified by using RNA-seq technology. In total, we found 1434 significant DEGs (SDEGs), which included 199 upregulated and 1235 downregulated genes in the C_B group compared with the B_B group. The immune system process GO term was the top significantly GO term, including MYD88, TLR4, IL15, VEGFA gene and so on. The cytokine-cytokine receptor interaction pathway and the Toll-like receptor signaling pathway were the key pathways affected by stress. The protein-protein interaction (PPI) analysis of the SDEGs showed that VEGFA, MyD88 and IL15 were hub genes and module analysis showed that MYD88, TLR4 and VEGFA play important roles in response to stress. CONCLUSION: This study showed that the VEGFA and ILs (such as IL15) via the cytokine-cytokine receptor interaction pathway, MYD88 and TLR4 via the Toll-like receptor signaling pathway may play important roles in the regulation of immune function under stress condition with CORT administration. The results of this study provide a reference for further studies of the molecular mechanisms of stress-influenced immune function. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5333-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-62936262018-12-18 Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model Zhang, Yanhua Zhou, Yanting Sun, Guirong Li, Kui Li, Zhuanjian Su, Aru Liu, Xiaojun Li, Guoxi Jiang, Ruirui Han, Ruili Tian, Yadong Kang, Xiangtao Yan, Fengbin BMC Genomics Research Article BACKGROUND: The molecular mechanisms underlying stress-influenced immune function of chicken (Gallus Gallus) are not clear. The stress models can be established effectively by feeding chickens corticosterone (CORT) hormone. The bursa of Fabricius is a unique central immune organ of birds. RNA-Seq technology was used to investigate differences in the expression profiles of immune-related genes and associated pathways in the bursa of Fabricius to clarify molecular mechanisms. The aim of this study was to broaden the understanding of the stress-influenced immune function in chickens. RESULTS: Differentially expressed genes (DEGs) in the bursa of Fabricius between experimental group (basal diet with added CORT 30 mg/kg; C_B group) and control group (basal diet; B_B group) were identified by using RNA-seq technology. In total, we found 1434 significant DEGs (SDEGs), which included 199 upregulated and 1235 downregulated genes in the C_B group compared with the B_B group. The immune system process GO term was the top significantly GO term, including MYD88, TLR4, IL15, VEGFA gene and so on. The cytokine-cytokine receptor interaction pathway and the Toll-like receptor signaling pathway were the key pathways affected by stress. The protein-protein interaction (PPI) analysis of the SDEGs showed that VEGFA, MyD88 and IL15 were hub genes and module analysis showed that MYD88, TLR4 and VEGFA play important roles in response to stress. CONCLUSION: This study showed that the VEGFA and ILs (such as IL15) via the cytokine-cytokine receptor interaction pathway, MYD88 and TLR4 via the Toll-like receptor signaling pathway may play important roles in the regulation of immune function under stress condition with CORT administration. The results of this study provide a reference for further studies of the molecular mechanisms of stress-influenced immune function. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5333-2) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-13 /pmc/articles/PMC6293626/ /pubmed/30545299 http://dx.doi.org/10.1186/s12864-018-5333-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Yanhua
Zhou, Yanting
Sun, Guirong
Li, Kui
Li, Zhuanjian
Su, Aru
Liu, Xiaojun
Li, Guoxi
Jiang, Ruirui
Han, Ruili
Tian, Yadong
Kang, Xiangtao
Yan, Fengbin
Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title_full Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title_fullStr Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title_full_unstemmed Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title_short Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model
title_sort transcriptome profile in bursa of fabricius reveals potential mode for stress-influenced immune function in chicken stress model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293626/
https://www.ncbi.nlm.nih.gov/pubmed/30545299
http://dx.doi.org/10.1186/s12864-018-5333-2
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