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Alterations of HDL Particles in Children with End-stage Renal Disease
BACKGROUND: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. I...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294087/ https://www.ncbi.nlm.nih.gov/pubmed/30581333 http://dx.doi.org/10.1515/jomb-2017-0019 |
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author | Stefanović, Aleksandra Ristovski-Kornic, Danijela Kotur-Stevuljević, Jelena Spasojević-Kalimanovska, Vesna Vekić, Jelena Miljković, Milica Paripović, Dusan Peco-Antic, Amira Jelić-Ivanović, Zorana Zeljković, Aleksandra |
author_facet | Stefanović, Aleksandra Ristovski-Kornic, Danijela Kotur-Stevuljević, Jelena Spasojević-Kalimanovska, Vesna Vekić, Jelena Miljković, Milica Paripović, Dusan Peco-Antic, Amira Jelić-Ivanović, Zorana Zeljković, Aleksandra |
author_sort | Stefanović, Aleksandra |
collection | PubMed |
description | BACKGROUND: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. METHODS: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). RESULTS: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses when compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. CONCLUSION: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality are not routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information. |
format | Online Article Text |
id | pubmed-6294087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-62940872018-12-21 Alterations of HDL Particles in Children with End-stage Renal Disease Stefanović, Aleksandra Ristovski-Kornic, Danijela Kotur-Stevuljević, Jelena Spasojević-Kalimanovska, Vesna Vekić, Jelena Miljković, Milica Paripović, Dusan Peco-Antic, Amira Jelić-Ivanović, Zorana Zeljković, Aleksandra J Med Biochem Original Paper BACKGROUND: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. METHODS: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). RESULTS: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses when compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. CONCLUSION: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality are not routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information. Sciendo 2017-10-28 /pmc/articles/PMC6294087/ /pubmed/30581333 http://dx.doi.org/10.1515/jomb-2017-0019 Text en © 2017 Aleksandra Stefanovič, Danijela Ristovski-Kornic, Jelena Kotur-Stevuljevič, Vesna Spasojevič-Kalimanovska, Jelena Vekič, Milica Miljkovič, Dusan Paripovič, Amira Peco-Antic, Zorana Jelič-Ivanovič, Aleksandra Zeljkovič, published by Sciendo http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. |
spellingShingle | Original Paper Stefanović, Aleksandra Ristovski-Kornic, Danijela Kotur-Stevuljević, Jelena Spasojević-Kalimanovska, Vesna Vekić, Jelena Miljković, Milica Paripović, Dusan Peco-Antic, Amira Jelić-Ivanović, Zorana Zeljković, Aleksandra Alterations of HDL Particles in Children with End-stage Renal Disease |
title | Alterations of HDL Particles in Children with End-stage Renal Disease |
title_full | Alterations of HDL Particles in Children with End-stage Renal Disease |
title_fullStr | Alterations of HDL Particles in Children with End-stage Renal Disease |
title_full_unstemmed | Alterations of HDL Particles in Children with End-stage Renal Disease |
title_short | Alterations of HDL Particles in Children with End-stage Renal Disease |
title_sort | alterations of hdl particles in children with end-stage renal disease |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294087/ https://www.ncbi.nlm.nih.gov/pubmed/30581333 http://dx.doi.org/10.1515/jomb-2017-0019 |
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