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The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells
A growing body of research supports the idea that the fallopian tube epithelium (FTE) is the precursor for most high-grade serous ovarian cancers (HGSOCs) but that the ovary plays a critical role in tumor metastasis. Cortical inclusion cysts (CICs) in the ovarian cortex have been hypothesized to cre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294138/ https://www.ncbi.nlm.nih.gov/pubmed/30556046 http://dx.doi.org/10.1063/1.5022595 |
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author | Fleszar, Andrew J. Walker, Alyssa Porubsky, Veronica Flanigan, Will James, Darian Campagnola, Paul J. Weisman, Paul S. Kreeger, Pamela K. |
author_facet | Fleszar, Andrew J. Walker, Alyssa Porubsky, Veronica Flanigan, Will James, Darian Campagnola, Paul J. Weisman, Paul S. Kreeger, Pamela K. |
author_sort | Fleszar, Andrew J. |
collection | PubMed |
description | A growing body of research supports the idea that the fallopian tube epithelium (FTE) is the precursor for most high-grade serous ovarian cancers (HGSOCs) but that the ovary plays a critical role in tumor metastasis. Cortical inclusion cysts (CICs) in the ovarian cortex have been hypothesized to create a niche environment that plays a role in HGSOC progression. Through histological analysis of pathology samples from human ovaries, we determined that collagen I and III were elevated near CICs and that the collagen fibers in this dense region were oriented parallel to the cyst boundary. Using this information from human samples as design parameters, we engineered an in vitro model that recreates the size, shape, and extracellular matrix properties of CICs. We found that FTE cells within our model underwent robust invasion that was responsive to stimulation with follicular fluid, while ovarian surface epithelial cells, the native cells of the ovary, were not invasive. We provide experimental evidence to support a role of the extracellular matrix in modulating FTE cell invasion, as a decrease in collagen I concentration or the addition of collagen III to the matrix surrounding FTE cells increased FTE cell invasion. Taken together, we show that an in vitro model of CICs obtained from the analysis of human tissue can act as an important tool for understanding FTE cell interactions with their environment. |
format | Online Article Text |
id | pubmed-6294138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-62941382018-12-14 The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells Fleszar, Andrew J. Walker, Alyssa Porubsky, Veronica Flanigan, Will James, Darian Campagnola, Paul J. Weisman, Paul S. Kreeger, Pamela K. APL Bioeng Special Topic: Bioengineering of Cancer A growing body of research supports the idea that the fallopian tube epithelium (FTE) is the precursor for most high-grade serous ovarian cancers (HGSOCs) but that the ovary plays a critical role in tumor metastasis. Cortical inclusion cysts (CICs) in the ovarian cortex have been hypothesized to create a niche environment that plays a role in HGSOC progression. Through histological analysis of pathology samples from human ovaries, we determined that collagen I and III were elevated near CICs and that the collagen fibers in this dense region were oriented parallel to the cyst boundary. Using this information from human samples as design parameters, we engineered an in vitro model that recreates the size, shape, and extracellular matrix properties of CICs. We found that FTE cells within our model underwent robust invasion that was responsive to stimulation with follicular fluid, while ovarian surface epithelial cells, the native cells of the ovary, were not invasive. We provide experimental evidence to support a role of the extracellular matrix in modulating FTE cell invasion, as a decrease in collagen I concentration or the addition of collagen III to the matrix surrounding FTE cells increased FTE cell invasion. Taken together, we show that an in vitro model of CICs obtained from the analysis of human tissue can act as an important tool for understanding FTE cell interactions with their environment. AIP Publishing LLC 2018-04-11 /pmc/articles/PMC6294138/ /pubmed/30556046 http://dx.doi.org/10.1063/1.5022595 Text en © 2018 Author(s). 2473-2877/2018/2(3)/031902/15 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Special Topic: Bioengineering of Cancer Fleszar, Andrew J. Walker, Alyssa Porubsky, Veronica Flanigan, Will James, Darian Campagnola, Paul J. Weisman, Paul S. Kreeger, Pamela K. The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title | The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title_full | The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title_fullStr | The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title_full_unstemmed | The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title_short | The extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
title_sort | extracellular matrix of ovarian cortical inclusion cysts modulates invasion of fallopian tube epithelial cells |
topic | Special Topic: Bioengineering of Cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294138/ https://www.ncbi.nlm.nih.gov/pubmed/30556046 http://dx.doi.org/10.1063/1.5022595 |
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