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How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb
Intrauterine crowding (IUC) considerably influences postnatal traits in a polytocous species such as the pig. Previously, our group described how IUC affects locomotion during the piglet’s first days of life (until 96 h after birth). We noted a reduced motor performance in piglets with a low birth w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294349/ https://www.ncbi.nlm.nih.gov/pubmed/30550550 http://dx.doi.org/10.1371/journal.pone.0209233 |
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author | Vanden Hole, Charlotte Cleuren, Silke Van Ginneken, Chris Prims, Sara Ayuso, Miriam Van Cruchten, Steven Aerts, Peter |
author_facet | Vanden Hole, Charlotte Cleuren, Silke Van Ginneken, Chris Prims, Sara Ayuso, Miriam Van Cruchten, Steven Aerts, Peter |
author_sort | Vanden Hole, Charlotte |
collection | PubMed |
description | Intrauterine crowding (IUC) considerably influences postnatal traits in a polytocous species such as the pig. Previously, our group described how IUC affects locomotion during the piglet’s first days of life (until 96 h after birth). We noted a reduced motor performance in piglets with a low birth weight and low vitality (L piglets), compared to piglets with a normal birth weight and normal vitality (N piglets), indicating L piglets are unable to produce enough force. Our current study investigates whether this observed force deficit in L piglets is caused by a reduced force generating capacity in the muscles and/or a lower percentage of type II (fast-contracting) fibers. Volume and fiber length of the main extensor muscles of the hind limb were used to estimate the physiological cross-sectional area (PSCA) and hence calculate the maximal isometric force generating capacity (F(iso-max)) of the hind limb. To check for developmental differences between the muscles of L and N piglets, F(iso-max) was normalized to body weight (BW), thus yielding a dimensionless variable F’(iso-max). To check for differences in muscle composition, m. vastus lateralis was stained immunohistochemically in order to determine the percentage of type II fibers through image analysis. Our results indicate that L piglets have a reduced absolute force generating capacity due to a lesser muscle mass, compared to N piglets. However, when normalized to BW L piglets actually show a larger force generating capacity, suggesting their muscles are more voluminous, given their body mass, than those of N piglets. However, no differences between L and N piglets were detected with regard to muscle composition of the m. vastus lateralis. Based on our data, we can say that neither normalized force generating capacity, nor muscle composition (of the m. vastus lateralis) can explain the observed force deficit in L piglets and as such the effect of IUC on locomotor performance. |
format | Online Article Text |
id | pubmed-6294349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62943492018-12-28 How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb Vanden Hole, Charlotte Cleuren, Silke Van Ginneken, Chris Prims, Sara Ayuso, Miriam Van Cruchten, Steven Aerts, Peter PLoS One Research Article Intrauterine crowding (IUC) considerably influences postnatal traits in a polytocous species such as the pig. Previously, our group described how IUC affects locomotion during the piglet’s first days of life (until 96 h after birth). We noted a reduced motor performance in piglets with a low birth weight and low vitality (L piglets), compared to piglets with a normal birth weight and normal vitality (N piglets), indicating L piglets are unable to produce enough force. Our current study investigates whether this observed force deficit in L piglets is caused by a reduced force generating capacity in the muscles and/or a lower percentage of type II (fast-contracting) fibers. Volume and fiber length of the main extensor muscles of the hind limb were used to estimate the physiological cross-sectional area (PSCA) and hence calculate the maximal isometric force generating capacity (F(iso-max)) of the hind limb. To check for developmental differences between the muscles of L and N piglets, F(iso-max) was normalized to body weight (BW), thus yielding a dimensionless variable F’(iso-max). To check for differences in muscle composition, m. vastus lateralis was stained immunohistochemically in order to determine the percentage of type II fibers through image analysis. Our results indicate that L piglets have a reduced absolute force generating capacity due to a lesser muscle mass, compared to N piglets. However, when normalized to BW L piglets actually show a larger force generating capacity, suggesting their muscles are more voluminous, given their body mass, than those of N piglets. However, no differences between L and N piglets were detected with regard to muscle composition of the m. vastus lateralis. Based on our data, we can say that neither normalized force generating capacity, nor muscle composition (of the m. vastus lateralis) can explain the observed force deficit in L piglets and as such the effect of IUC on locomotor performance. Public Library of Science 2018-12-14 /pmc/articles/PMC6294349/ /pubmed/30550550 http://dx.doi.org/10.1371/journal.pone.0209233 Text en © 2018 Vanden Hole et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vanden Hole, Charlotte Cleuren, Silke Van Ginneken, Chris Prims, Sara Ayuso, Miriam Van Cruchten, Steven Aerts, Peter How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title | How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title_full | How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title_fullStr | How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title_full_unstemmed | How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title_short | How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb |
title_sort | how does intrauterine crowding affect locomotor performance in newborn pigs? a study of force generating capacity and muscle composition of the hind limb |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294349/ https://www.ncbi.nlm.nih.gov/pubmed/30550550 http://dx.doi.org/10.1371/journal.pone.0209233 |
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