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Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML
CD25 is expressed on leukemic cells in 10–20% cases of acute myeloid leukemia (AML), and its expression is associated with poor prognosis. We reevaluated the relationship between CD25 expression and the leukemia-initiating cell (LIC) properties of AML using a patient-derived xenograft model. We divi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294374/ https://www.ncbi.nlm.nih.gov/pubmed/30550585 http://dx.doi.org/10.1371/journal.pone.0209295 |
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author | Kageyama, Yuki Miwa, Hiroshi Arakawa, Rino Tawara, Isao Ohishi, Kohshi Masuya, Masahiro Nakase, Kazunori Katayama, Naoyuki |
author_facet | Kageyama, Yuki Miwa, Hiroshi Arakawa, Rino Tawara, Isao Ohishi, Kohshi Masuya, Masahiro Nakase, Kazunori Katayama, Naoyuki |
author_sort | Kageyama, Yuki |
collection | PubMed |
description | CD25 is expressed on leukemic cells in 10–20% cases of acute myeloid leukemia (AML), and its expression is associated with poor prognosis. We reevaluated the relationship between CD25 expression and the leukemia-initiating cell (LIC) properties of AML using a patient-derived xenograft model. We divided lineage marker-negative (Lin(–)) CD34(+)CD38(–) or Lin(–)CD34(+) cells from CD25-positive AML into CD25-positive and -negative populations, and then transplanted each population into NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/Sz mice. Leukemic engraftment was observed with both CD25-positive and -negative populations from three of nine CD25-positive AML patients. In two of those three patients, CD25-positive and -negative Lin(–)CD34(+) cells engrafted at the primary transplantation led to leukemic engraftment at the secondary transplantation, in which engrafted cells contained both CD25-positive and -negative Lin(–)CD34(+) AML cells. In an in vitro culture system, expression of CD25 was considerably induced in the CD25-negative population of Lin(–)CD34(+) cells from two cases of CD25-positive AML. In one case, CD25-positive Lin(–)CD34(+) cells gave rise to CD25-negative as well as -positive CD34(+) cells. These observations suggest that there exist CD25-positive and -negative populations that can reconstitute CD25-positive AML in a patient-derived xenograft model, and that CD25 expression fluctuates in the LICs of AML. |
format | Online Article Text |
id | pubmed-6294374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62943742018-12-28 Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML Kageyama, Yuki Miwa, Hiroshi Arakawa, Rino Tawara, Isao Ohishi, Kohshi Masuya, Masahiro Nakase, Kazunori Katayama, Naoyuki PLoS One Research Article CD25 is expressed on leukemic cells in 10–20% cases of acute myeloid leukemia (AML), and its expression is associated with poor prognosis. We reevaluated the relationship between CD25 expression and the leukemia-initiating cell (LIC) properties of AML using a patient-derived xenograft model. We divided lineage marker-negative (Lin(–)) CD34(+)CD38(–) or Lin(–)CD34(+) cells from CD25-positive AML into CD25-positive and -negative populations, and then transplanted each population into NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/Sz mice. Leukemic engraftment was observed with both CD25-positive and -negative populations from three of nine CD25-positive AML patients. In two of those three patients, CD25-positive and -negative Lin(–)CD34(+) cells engrafted at the primary transplantation led to leukemic engraftment at the secondary transplantation, in which engrafted cells contained both CD25-positive and -negative Lin(–)CD34(+) AML cells. In an in vitro culture system, expression of CD25 was considerably induced in the CD25-negative population of Lin(–)CD34(+) cells from two cases of CD25-positive AML. In one case, CD25-positive Lin(–)CD34(+) cells gave rise to CD25-negative as well as -positive CD34(+) cells. These observations suggest that there exist CD25-positive and -negative populations that can reconstitute CD25-positive AML in a patient-derived xenograft model, and that CD25 expression fluctuates in the LICs of AML. Public Library of Science 2018-12-14 /pmc/articles/PMC6294374/ /pubmed/30550585 http://dx.doi.org/10.1371/journal.pone.0209295 Text en © 2018 Kageyama et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kageyama, Yuki Miwa, Hiroshi Arakawa, Rino Tawara, Isao Ohishi, Kohshi Masuya, Masahiro Nakase, Kazunori Katayama, Naoyuki Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title | Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title_full | Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title_fullStr | Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title_full_unstemmed | Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title_short | Expression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML |
title_sort | expression of cd25 fluctuates in the leukemia-initiating cell population of cd25-positive aml |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294374/ https://www.ncbi.nlm.nih.gov/pubmed/30550585 http://dx.doi.org/10.1371/journal.pone.0209295 |
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