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Effect of CFIm25 knockout on RNA polymerase II transcription

OBJECTIVES: Transcription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is a highly regulated process. Most human genes have multiple poly(A) sites, which define different possible mRNA ends, suggesting the existence of mechanisms that regulate which poly(A) site is used. Poly(A)...

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Autores principales: Tellier, Michael, Hardy, Jessica G., Norbury, Chris J., Murphy, Shona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295108/
https://www.ncbi.nlm.nih.gov/pubmed/30547832
http://dx.doi.org/10.1186/s13104-018-4006-1
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author Tellier, Michael
Hardy, Jessica G.
Norbury, Chris J.
Murphy, Shona
author_facet Tellier, Michael
Hardy, Jessica G.
Norbury, Chris J.
Murphy, Shona
author_sort Tellier, Michael
collection PubMed
description OBJECTIVES: Transcription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is a highly regulated process. Most human genes have multiple poly(A) sites, which define different possible mRNA ends, suggesting the existence of mechanisms that regulate which poly(A) site is used. Poly(A) site selection may be mediated by cleavage factor I (CFIm), which is part of the cleavage and polyadenylation (CPA) complex. CFIm comprises CFIm25, CFIm59 and CFim68 subunits. It has been documented that the CPA complex also regulates pol II transcription at the start of genes. We therefore investigated whether CFIm, in addition to its role in poly(A) site selection, is involved in the regulation of pol II transcription. DATA DESCRIPTION: We provide genome-wide data of the effect of reducing by 90% expression of the CFIm25 constituent of CFIm, which is involved in pre-mRNA cleavage and polyadenylation, on pol II transcription in human cells. We performed pol II ChIP-seq in the presence or absence of CFIm25 and with or without an inhibitor of the cyclin-dependent kinase (CDK)9, which regulates the entry of pol II into productive elongation.
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spelling pubmed-62951082018-12-18 Effect of CFIm25 knockout on RNA polymerase II transcription Tellier, Michael Hardy, Jessica G. Norbury, Chris J. Murphy, Shona BMC Res Notes Data Note OBJECTIVES: Transcription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is a highly regulated process. Most human genes have multiple poly(A) sites, which define different possible mRNA ends, suggesting the existence of mechanisms that regulate which poly(A) site is used. Poly(A) site selection may be mediated by cleavage factor I (CFIm), which is part of the cleavage and polyadenylation (CPA) complex. CFIm comprises CFIm25, CFIm59 and CFim68 subunits. It has been documented that the CPA complex also regulates pol II transcription at the start of genes. We therefore investigated whether CFIm, in addition to its role in poly(A) site selection, is involved in the regulation of pol II transcription. DATA DESCRIPTION: We provide genome-wide data of the effect of reducing by 90% expression of the CFIm25 constituent of CFIm, which is involved in pre-mRNA cleavage and polyadenylation, on pol II transcription in human cells. We performed pol II ChIP-seq in the presence or absence of CFIm25 and with or without an inhibitor of the cyclin-dependent kinase (CDK)9, which regulates the entry of pol II into productive elongation. BioMed Central 2018-12-14 /pmc/articles/PMC6295108/ /pubmed/30547832 http://dx.doi.org/10.1186/s13104-018-4006-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Data Note
Tellier, Michael
Hardy, Jessica G.
Norbury, Chris J.
Murphy, Shona
Effect of CFIm25 knockout on RNA polymerase II transcription
title Effect of CFIm25 knockout on RNA polymerase II transcription
title_full Effect of CFIm25 knockout on RNA polymerase II transcription
title_fullStr Effect of CFIm25 knockout on RNA polymerase II transcription
title_full_unstemmed Effect of CFIm25 knockout on RNA polymerase II transcription
title_short Effect of CFIm25 knockout on RNA polymerase II transcription
title_sort effect of cfim25 knockout on rna polymerase ii transcription
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295108/
https://www.ncbi.nlm.nih.gov/pubmed/30547832
http://dx.doi.org/10.1186/s13104-018-4006-1
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