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Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study

BACKGROUND: Angiogenesis plays a crucial role in myocardial infarction (MI) treatment by ameliorating myocardial remodeling, thus improving cardiac function and preventing heart failure. Muscone has been reported to have beneficial effects on cardiac remodeling in MI mice. However, the effects of mu...

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Autores principales: Du, Yingqiang, Ge, Yingbin, Xu, Zhihui, Aa, Nan, Gu, Xin, Meng, Haoyu, Lin, Zhou, Zhu, Dongxiao, Shi, Jingjing, Zhuang, Ruijuan, Wu, Xueming, Wang, Xiaoyan, Yang, Zhijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295139/
https://www.ncbi.nlm.nih.gov/pubmed/30531686
http://dx.doi.org/10.12659/MSM.912051
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author Du, Yingqiang
Ge, Yingbin
Xu, Zhihui
Aa, Nan
Gu, Xin
Meng, Haoyu
Lin, Zhou
Zhu, Dongxiao
Shi, Jingjing
Zhuang, Ruijuan
Wu, Xueming
Wang, Xiaoyan
Yang, Zhijian
author_facet Du, Yingqiang
Ge, Yingbin
Xu, Zhihui
Aa, Nan
Gu, Xin
Meng, Haoyu
Lin, Zhou
Zhu, Dongxiao
Shi, Jingjing
Zhuang, Ruijuan
Wu, Xueming
Wang, Xiaoyan
Yang, Zhijian
author_sort Du, Yingqiang
collection PubMed
description BACKGROUND: Angiogenesis plays a crucial role in myocardial infarction (MI) treatment by ameliorating myocardial remodeling, thus improving cardiac function and preventing heart failure. Muscone has been reported to have beneficial effects on cardiac remodeling in MI mice. However, the effects of muscone on angiogenesis in MI mice and its underlying mechanisms remain unknown. MATERIAL/METHODS: Mice were randomly divided into sham, MI, and MI+muscone groups. The MI mouse model was established by ligating the left anterior descending coronary artery. Mice in the sham group received the same procedure except for ligation. Mice were administered muscone or an equivalent volume of saline for 4 consecutive weeks. Cardiac function was evaluated by echocardiograph after MI for 2 and 4 weeks. Four weeks later, all mice were sacrificed and Masson’s trichrome staining was used to assess myocardial fibrosis. Isolectin B4 staining was applied to evaluate the angiogenesis in mouse hearts. Immunohistochemistry, Western blot analysis, and quantitative real-time polymerase chain reaction (qPCR) were performed to analyze expression levels of HIF-1α and its downstream genes. RESULTS: Compared with the MI group, muscone treatment significantly improved cardiac function and reduced myocardial fibrosis. Moreover, muscone enhanced angiogenesis in the peri-infarct region and p-VEGFR2 expression in the vascular endothelial cells. Western blot analysis and qPCR showed that muscone upregulated expression levels of HIF-1α and VEGFA. CONCLUSIONS: Muscone improved cardiac function in MI mice through augmented angiogenesis. The potential mechanism of muscone treatment in regulating angiogenesis of MI mice was upregulating expression levels of HIF-1α and VEGFA.
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spelling pubmed-62951392019-01-08 Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study Du, Yingqiang Ge, Yingbin Xu, Zhihui Aa, Nan Gu, Xin Meng, Haoyu Lin, Zhou Zhu, Dongxiao Shi, Jingjing Zhuang, Ruijuan Wu, Xueming Wang, Xiaoyan Yang, Zhijian Med Sci Monit Animal Study BACKGROUND: Angiogenesis plays a crucial role in myocardial infarction (MI) treatment by ameliorating myocardial remodeling, thus improving cardiac function and preventing heart failure. Muscone has been reported to have beneficial effects on cardiac remodeling in MI mice. However, the effects of muscone on angiogenesis in MI mice and its underlying mechanisms remain unknown. MATERIAL/METHODS: Mice were randomly divided into sham, MI, and MI+muscone groups. The MI mouse model was established by ligating the left anterior descending coronary artery. Mice in the sham group received the same procedure except for ligation. Mice were administered muscone or an equivalent volume of saline for 4 consecutive weeks. Cardiac function was evaluated by echocardiograph after MI for 2 and 4 weeks. Four weeks later, all mice were sacrificed and Masson’s trichrome staining was used to assess myocardial fibrosis. Isolectin B4 staining was applied to evaluate the angiogenesis in mouse hearts. Immunohistochemistry, Western blot analysis, and quantitative real-time polymerase chain reaction (qPCR) were performed to analyze expression levels of HIF-1α and its downstream genes. RESULTS: Compared with the MI group, muscone treatment significantly improved cardiac function and reduced myocardial fibrosis. Moreover, muscone enhanced angiogenesis in the peri-infarct region and p-VEGFR2 expression in the vascular endothelial cells. Western blot analysis and qPCR showed that muscone upregulated expression levels of HIF-1α and VEGFA. CONCLUSIONS: Muscone improved cardiac function in MI mice through augmented angiogenesis. The potential mechanism of muscone treatment in regulating angiogenesis of MI mice was upregulating expression levels of HIF-1α and VEGFA. International Scientific Literature, Inc. 2018-12-08 /pmc/articles/PMC6295139/ /pubmed/30531686 http://dx.doi.org/10.12659/MSM.912051 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Animal Study
Du, Yingqiang
Ge, Yingbin
Xu, Zhihui
Aa, Nan
Gu, Xin
Meng, Haoyu
Lin, Zhou
Zhu, Dongxiao
Shi, Jingjing
Zhuang, Ruijuan
Wu, Xueming
Wang, Xiaoyan
Yang, Zhijian
Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title_full Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title_fullStr Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title_full_unstemmed Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title_short Hypoxia-Inducible Factor 1 alpha (HIF-1α)/Vascular Endothelial Growth Factor (VEGF) Pathway Participates in Angiogenesis of Myocardial Infarction in Muscone-Treated Mice: Preliminary Study
title_sort hypoxia-inducible factor 1 alpha (hif-1α)/vascular endothelial growth factor (vegf) pathway participates in angiogenesis of myocardial infarction in muscone-treated mice: preliminary study
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295139/
https://www.ncbi.nlm.nih.gov/pubmed/30531686
http://dx.doi.org/10.12659/MSM.912051
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