FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function

FLIRT (Fast Local InfraRed Thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function during cellular behaviors while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature sensitive (ts) mutant proteins, using non...

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Detalles Bibliográficos
Autores principales: Hirsch, Sophia M., Sundaramoorthy, Sriramkumar, Davies, Tim, Zhuravlev, Yelena, Waters, Jennifer C., Shirasu-Hiza, Mimi, Dumont, Julien, Canman, Julie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295154/
https://www.ncbi.nlm.nih.gov/pubmed/30377360
http://dx.doi.org/10.1038/s41592-018-0168-y
Descripción
Sumario:FLIRT (Fast Local InfraRed Thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function during cellular behaviors while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature sensitive (ts) mutant proteins, using non-ts mutants as controls. We demonstrate that FLIRT can control ts proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in C. elegans with cell-specific and even subcellular precision.

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