FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function

FLIRT (Fast Local InfraRed Thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function during cellular behaviors while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature sensitive (ts) mutant proteins, using non...

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Autores principales: Hirsch, Sophia M., Sundaramoorthy, Sriramkumar, Davies, Tim, Zhuravlev, Yelena, Waters, Jennifer C., Shirasu-Hiza, Mimi, Dumont, Julien, Canman, Julie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295154/
https://www.ncbi.nlm.nih.gov/pubmed/30377360
http://dx.doi.org/10.1038/s41592-018-0168-y
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author Hirsch, Sophia M.
Sundaramoorthy, Sriramkumar
Davies, Tim
Zhuravlev, Yelena
Waters, Jennifer C.
Shirasu-Hiza, Mimi
Dumont, Julien
Canman, Julie C.
author_facet Hirsch, Sophia M.
Sundaramoorthy, Sriramkumar
Davies, Tim
Zhuravlev, Yelena
Waters, Jennifer C.
Shirasu-Hiza, Mimi
Dumont, Julien
Canman, Julie C.
author_sort Hirsch, Sophia M.
collection PubMed
description FLIRT (Fast Local InfraRed Thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function during cellular behaviors while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature sensitive (ts) mutant proteins, using non-ts mutants as controls. We demonstrate that FLIRT can control ts proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in C. elegans with cell-specific and even subcellular precision.
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spelling pubmed-62951542019-04-30 FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function Hirsch, Sophia M. Sundaramoorthy, Sriramkumar Davies, Tim Zhuravlev, Yelena Waters, Jennifer C. Shirasu-Hiza, Mimi Dumont, Julien Canman, Julie C. Nat Methods Article FLIRT (Fast Local InfraRed Thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function during cellular behaviors while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature sensitive (ts) mutant proteins, using non-ts mutants as controls. We demonstrate that FLIRT can control ts proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in C. elegans with cell-specific and even subcellular precision. 2018-10-30 2018-11 /pmc/articles/PMC6295154/ /pubmed/30377360 http://dx.doi.org/10.1038/s41592-018-0168-y Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hirsch, Sophia M.
Sundaramoorthy, Sriramkumar
Davies, Tim
Zhuravlev, Yelena
Waters, Jennifer C.
Shirasu-Hiza, Mimi
Dumont, Julien
Canman, Julie C.
FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title_full FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title_fullStr FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title_full_unstemmed FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title_short FLIRT: Fast Local InfraRed Thermogenetics for subcellular control of protein function
title_sort flirt: fast local infrared thermogenetics for subcellular control of protein function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295154/
https://www.ncbi.nlm.nih.gov/pubmed/30377360
http://dx.doi.org/10.1038/s41592-018-0168-y
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