Cargando…

Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures

A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compa...

Descripción completa

Detalles Bibliográficos
Autores principales: Morales-Soto, Nydia, Cao, Tianyuan, Baig, Nameera F, Kramer, Kristen M, Bohn, Paul W, Shrout, Joshua D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295745/
https://www.ncbi.nlm.nih.gov/pubmed/30573968
http://dx.doi.org/10.1177/1178636118817738
_version_ 1783380920741396480
author Morales-Soto, Nydia
Cao, Tianyuan
Baig, Nameera F
Kramer, Kristen M
Bohn, Paul W
Shrout, Joshua D
author_facet Morales-Soto, Nydia
Cao, Tianyuan
Baig, Nameera F
Kramer, Kristen M
Bohn, Paul W
Shrout, Joshua D
author_sort Morales-Soto, Nydia
collection PubMed
description A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compare variations in the alkyl quinolone (AQ) family of molecules during the transition between surface-attached motile-swarming and stationary biofilm communities. The AQs have been established previously as important to Pseudomonas aeruginosa biofilms, interspecies competition, and virulence. The AQ Pseudomonas quinolone signal (PQS) is also a known quorum-sensing signal. We detail spatial identification of AQ, PQS, and 2-alkyl-4-hydroxyquinoline N-oxide (AQNO) metabolites in both swarm and biofilm communities. We find that AQNO metabolites are abundant signatures in active swarming communities.
format Online
Article
Text
id pubmed-6295745
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-62957452018-12-20 Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures Morales-Soto, Nydia Cao, Tianyuan Baig, Nameera F Kramer, Kristen M Bohn, Paul W Shrout, Joshua D Microbiol Insights Brief Report A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compare variations in the alkyl quinolone (AQ) family of molecules during the transition between surface-attached motile-swarming and stationary biofilm communities. The AQs have been established previously as important to Pseudomonas aeruginosa biofilms, interspecies competition, and virulence. The AQ Pseudomonas quinolone signal (PQS) is also a known quorum-sensing signal. We detail spatial identification of AQ, PQS, and 2-alkyl-4-hydroxyquinoline N-oxide (AQNO) metabolites in both swarm and biofilm communities. We find that AQNO metabolites are abundant signatures in active swarming communities. SAGE Publications 2018-12-16 /pmc/articles/PMC6295745/ /pubmed/30573968 http://dx.doi.org/10.1177/1178636118817738 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Report
Morales-Soto, Nydia
Cao, Tianyuan
Baig, Nameera F
Kramer, Kristen M
Bohn, Paul W
Shrout, Joshua D
Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title_full Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title_fullStr Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title_full_unstemmed Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title_short Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
title_sort surface-growing communities of pseudomonas aeruginosa exhibit distinct alkyl quinolone signatures
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295745/
https://www.ncbi.nlm.nih.gov/pubmed/30573968
http://dx.doi.org/10.1177/1178636118817738
work_keys_str_mv AT moralessotonydia surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures
AT caotianyuan surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures
AT baignameeraf surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures
AT kramerkristenm surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures
AT bohnpaulw surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures
AT shroutjoshuad surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures