Cargando…
Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures
A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compa...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295745/ https://www.ncbi.nlm.nih.gov/pubmed/30573968 http://dx.doi.org/10.1177/1178636118817738 |
_version_ | 1783380920741396480 |
---|---|
author | Morales-Soto, Nydia Cao, Tianyuan Baig, Nameera F Kramer, Kristen M Bohn, Paul W Shrout, Joshua D |
author_facet | Morales-Soto, Nydia Cao, Tianyuan Baig, Nameera F Kramer, Kristen M Bohn, Paul W Shrout, Joshua D |
author_sort | Morales-Soto, Nydia |
collection | PubMed |
description | A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compare variations in the alkyl quinolone (AQ) family of molecules during the transition between surface-attached motile-swarming and stationary biofilm communities. The AQs have been established previously as important to Pseudomonas aeruginosa biofilms, interspecies competition, and virulence. The AQ Pseudomonas quinolone signal (PQS) is also a known quorum-sensing signal. We detail spatial identification of AQ, PQS, and 2-alkyl-4-hydroxyquinoline N-oxide (AQNO) metabolites in both swarm and biofilm communities. We find that AQNO metabolites are abundant signatures in active swarming communities. |
format | Online Article Text |
id | pubmed-6295745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-62957452018-12-20 Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures Morales-Soto, Nydia Cao, Tianyuan Baig, Nameera F Kramer, Kristen M Bohn, Paul W Shrout, Joshua D Microbiol Insights Brief Report A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compare variations in the alkyl quinolone (AQ) family of molecules during the transition between surface-attached motile-swarming and stationary biofilm communities. The AQs have been established previously as important to Pseudomonas aeruginosa biofilms, interspecies competition, and virulence. The AQ Pseudomonas quinolone signal (PQS) is also a known quorum-sensing signal. We detail spatial identification of AQ, PQS, and 2-alkyl-4-hydroxyquinoline N-oxide (AQNO) metabolites in both swarm and biofilm communities. We find that AQNO metabolites are abundant signatures in active swarming communities. SAGE Publications 2018-12-16 /pmc/articles/PMC6295745/ /pubmed/30573968 http://dx.doi.org/10.1177/1178636118817738 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Brief Report Morales-Soto, Nydia Cao, Tianyuan Baig, Nameera F Kramer, Kristen M Bohn, Paul W Shrout, Joshua D Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title | Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title_full | Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title_fullStr | Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title_full_unstemmed | Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title_short | Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures |
title_sort | surface-growing communities of pseudomonas aeruginosa exhibit distinct alkyl quinolone signatures |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295745/ https://www.ncbi.nlm.nih.gov/pubmed/30573968 http://dx.doi.org/10.1177/1178636118817738 |
work_keys_str_mv | AT moralessotonydia surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures AT caotianyuan surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures AT baignameeraf surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures AT kramerkristenm surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures AT bohnpaulw surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures AT shroutjoshuad surfacegrowingcommunitiesofpseudomonasaeruginosaexhibitdistinctalkylquinolonesignatures |