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Whole-genome of Mexican-crAssphage isolated from the human gut microbiome

OBJECTIVES: crAssphage is a newly found phage described as the most abundant virus in the human gut microbiome. The majority of the crAssphage proteins are unknown in sequences databases, and its pathogenicity and epidemiology in humans are yet unclear. Hence, being one of the most abundant phages i...

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Autores principales: Cervantes-Echeverría, Melany, Equihua-Medina, Edgar, Cornejo-Granados, Fernanda, Hernández-Reyna, Abigail, Sánchez, Filiberto, López-Contreras, Blanca Estela, Canizales-Quinteros, Samuel, Ochoa-Leyva, Adrián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296078/
https://www.ncbi.nlm.nih.gov/pubmed/30558657
http://dx.doi.org/10.1186/s13104-018-4010-5
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author Cervantes-Echeverría, Melany
Equihua-Medina, Edgar
Cornejo-Granados, Fernanda
Hernández-Reyna, Abigail
Sánchez, Filiberto
López-Contreras, Blanca Estela
Canizales-Quinteros, Samuel
Ochoa-Leyva, Adrián
author_facet Cervantes-Echeverría, Melany
Equihua-Medina, Edgar
Cornejo-Granados, Fernanda
Hernández-Reyna, Abigail
Sánchez, Filiberto
López-Contreras, Blanca Estela
Canizales-Quinteros, Samuel
Ochoa-Leyva, Adrián
author_sort Cervantes-Echeverría, Melany
collection PubMed
description OBJECTIVES: crAssphage is a newly found phage described as the most abundant virus in the human gut microbiome. The majority of the crAssphage proteins are unknown in sequences databases, and its pathogenicity and epidemiology in humans are yet unclear. Hence, being one of the most abundant phages in the human gut microbiome more investigation at the genomic level is necessary to improve our understanding, especially in the Latin American population. DATA DESCRIPTION: In this article, we provide the whole genome of a crAssphage isolated from the human gut microbiome of the Mexican population, which was named Mexican-crAssphage. The genome consists of 96,283 bp, G+C content of 29.24% and 87 coding sequences. Notably, we did not find any transfer RNA genes in the genome sequence. We also sequenced viral-like enriched particles from 28 fecal samples, and we detected the presence of the Mexican-crAssphage genome in 8 samples (28.5%). To our knowledge, our data is the first whole genome report of the crAssphage isolated from the Latin American Population and provides valuable information for the experimental characterization of the most abundant human gut bacteriophage. The whole genome shotgun project of the Mexican-crAssphage is available at DDBJ/ENA/GenBank under the GenBank MK069403.
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spelling pubmed-62960782018-12-18 Whole-genome of Mexican-crAssphage isolated from the human gut microbiome Cervantes-Echeverría, Melany Equihua-Medina, Edgar Cornejo-Granados, Fernanda Hernández-Reyna, Abigail Sánchez, Filiberto López-Contreras, Blanca Estela Canizales-Quinteros, Samuel Ochoa-Leyva, Adrián BMC Res Notes Data Note OBJECTIVES: crAssphage is a newly found phage described as the most abundant virus in the human gut microbiome. The majority of the crAssphage proteins are unknown in sequences databases, and its pathogenicity and epidemiology in humans are yet unclear. Hence, being one of the most abundant phages in the human gut microbiome more investigation at the genomic level is necessary to improve our understanding, especially in the Latin American population. DATA DESCRIPTION: In this article, we provide the whole genome of a crAssphage isolated from the human gut microbiome of the Mexican population, which was named Mexican-crAssphage. The genome consists of 96,283 bp, G+C content of 29.24% and 87 coding sequences. Notably, we did not find any transfer RNA genes in the genome sequence. We also sequenced viral-like enriched particles from 28 fecal samples, and we detected the presence of the Mexican-crAssphage genome in 8 samples (28.5%). To our knowledge, our data is the first whole genome report of the crAssphage isolated from the Latin American Population and provides valuable information for the experimental characterization of the most abundant human gut bacteriophage. The whole genome shotgun project of the Mexican-crAssphage is available at DDBJ/ENA/GenBank under the GenBank MK069403. BioMed Central 2018-12-17 /pmc/articles/PMC6296078/ /pubmed/30558657 http://dx.doi.org/10.1186/s13104-018-4010-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Data Note
Cervantes-Echeverría, Melany
Equihua-Medina, Edgar
Cornejo-Granados, Fernanda
Hernández-Reyna, Abigail
Sánchez, Filiberto
López-Contreras, Blanca Estela
Canizales-Quinteros, Samuel
Ochoa-Leyva, Adrián
Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title_full Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title_fullStr Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title_full_unstemmed Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title_short Whole-genome of Mexican-crAssphage isolated from the human gut microbiome
title_sort whole-genome of mexican-crassphage isolated from the human gut microbiome
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296078/
https://www.ncbi.nlm.nih.gov/pubmed/30558657
http://dx.doi.org/10.1186/s13104-018-4010-5
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