First study of sperm mediated gene transfer in Egyptian river buffalo

The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmi...

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Autores principales: Hassanane, Mohamed S., El Makawy, Aida I., Helalia, Sahar M., Abdoon, Ahmed S., Khalil, Kamal M.A., Ghanem, Tharwat A., Tohamy, Amany M., Sun, Xiao-Feng, Shen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2017
Materias:
Animal Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296624/
https://www.ncbi.nlm.nih.gov/pubmed/30647689
http://dx.doi.org/10.1016/j.jgeb.2017.06.003
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author Hassanane, Mohamed S.
El Makawy, Aida I.
Helalia, Sahar M.
Abdoon, Ahmed S.
Khalil, Kamal M.A.
Ghanem, Tharwat A.
Tohamy, Amany M.
Sun, Xiao-Feng
Shen, Wei
author_facet Hassanane, Mohamed S.
El Makawy, Aida I.
Helalia, Sahar M.
Abdoon, Ahmed S.
Khalil, Kamal M.A.
Ghanem, Tharwat A.
Tohamy, Amany M.
Sun, Xiao-Feng
Shen, Wei
author_sort Hassanane, Mohamed S.
collection PubMed
description The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfecting agent concentration: Dimethyle sulphoxide (DMSO) and time of transfection. The study proved that the best conditions for producing transgenic embryos were incubation sperm solution its concentration is 10(7)/ml sperm with 3% DMSO: with 20 µg/ml from the linarized DNA, for 15 min at 4 °C are the best conditions to produce transgenic buffalo embryo using sperm mediated gene transfer.
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spelling pubmed-62966242019-01-15 First study of sperm mediated gene transfer in Egyptian river buffalo Hassanane, Mohamed S. El Makawy, Aida I. Helalia, Sahar M. Abdoon, Ahmed S. Khalil, Kamal M.A. Ghanem, Tharwat A. Tohamy, Amany M. Sun, Xiao-Feng Shen, Wei J Genet Eng Biotechnol Animal Biotechnology The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfecting agent concentration: Dimethyle sulphoxide (DMSO) and time of transfection. The study proved that the best conditions for producing transgenic embryos were incubation sperm solution its concentration is 10(7)/ml sperm with 3% DMSO: with 20 µg/ml from the linarized DNA, for 15 min at 4 °C are the best conditions to produce transgenic buffalo embryo using sperm mediated gene transfer. Academy of Scientific Research and Technology, Egypt 2017-12 2017-06-22 /pmc/articles/PMC6296624/ /pubmed/30647689 http://dx.doi.org/10.1016/j.jgeb.2017.06.003 Text en © 2017 Production and hosting by Elsevier B.V. on behalf of Academy of Scientific Research & Technology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Animal Biotechnology
Hassanane, Mohamed S.
El Makawy, Aida I.
Helalia, Sahar M.
Abdoon, Ahmed S.
Khalil, Kamal M.A.
Ghanem, Tharwat A.
Tohamy, Amany M.
Sun, Xiao-Feng
Shen, Wei
First study of sperm mediated gene transfer in Egyptian river buffalo
title First study of sperm mediated gene transfer in Egyptian river buffalo
title_full First study of sperm mediated gene transfer in Egyptian river buffalo
title_fullStr First study of sperm mediated gene transfer in Egyptian river buffalo
title_full_unstemmed First study of sperm mediated gene transfer in Egyptian river buffalo
title_short First study of sperm mediated gene transfer in Egyptian river buffalo
title_sort first study of sperm mediated gene transfer in egyptian river buffalo
topic Animal Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296624/
https://www.ncbi.nlm.nih.gov/pubmed/30647689
http://dx.doi.org/10.1016/j.jgeb.2017.06.003
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