Cargando…

Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract

INTRODUCTION: There is emerging interest in medicinal plants in the biomedical field, due to their multitude of chemicals which show anti-inflammatory, antimicrobial, antiviral, or antitumoral potential. Research on medicinal plants has shown that nanotechnology could offer new solutions in the qual...

Descripción completa

Detalles Bibliográficos
Autores principales: Kedi, Philippe Belle Ebanda, Meva, Francois Eya’ane, Kotsedi, Lebogang, Nguemfo, Edwige Laure, Zangueu, Calvin Bogning, Ntoumba, Agnes Antoinette, Mohamed, Hamza Elsayed Ahmed, Dongmo, Alain Bertrand, Maaza, Malik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296690/
https://www.ncbi.nlm.nih.gov/pubmed/30587976
http://dx.doi.org/10.2147/IJN.S174530
_version_ 1783381096403042304
author Kedi, Philippe Belle Ebanda
Meva, Francois Eya’ane
Kotsedi, Lebogang
Nguemfo, Edwige Laure
Zangueu, Calvin Bogning
Ntoumba, Agnes Antoinette
Mohamed, Hamza Elsayed Ahmed
Dongmo, Alain Bertrand
Maaza, Malik
author_facet Kedi, Philippe Belle Ebanda
Meva, Francois Eya’ane
Kotsedi, Lebogang
Nguemfo, Edwige Laure
Zangueu, Calvin Bogning
Ntoumba, Agnes Antoinette
Mohamed, Hamza Elsayed Ahmed
Dongmo, Alain Bertrand
Maaza, Malik
author_sort Kedi, Philippe Belle Ebanda
collection PubMed
description INTRODUCTION: There is emerging interest in medicinal plants in the biomedical field, due to their multitude of chemicals which show anti-inflammatory, antimicrobial, antiviral, or antitumoral potential. Research on medicinal plants has shown that nanotechnology could offer new solutions in the quality control, delivery aspects, or in sustaining herbal biological activities. This work reports on the preparation and characterization of silver nanoparticle-mediated Selaginella myosurus plant extract. METHODS: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, energy dispersive X-ray spectroscopy, high-resolution scanning electron microscopy, high-resolution transmission electron microscopy, and selected area electron diffraction have been used to characterize the prepared silver nanoparticles. The synthetic stability was studied by varying concentrations and pH of reactants. Egg albumin denaturation and carrageenan-induced rat paw edema model were used to ascertain the anti-inflammation. RESULTS: Ultraviolet-visible spectroscopy gave plasmon resonance ranging between 420 and 480 nm while Fourier transform infrared spectroscopy proved nano interface functionalized with organics. The powder X-ray diffraction pattern is in agreement with silver and silver chloride nanoparticles of crystallite size 33.7 nm and 44.2 nm for silver and silver chloride, respectively. Energy dispersive X-ray spectroscopy enables elemental characterization of the particles consisting of silver and silver chloride among main elements. Spherical silver grain of 58.81 nm average size has been depicted with high-resolution scanning electron microscopy and high-resolution transmission electron microscopy. Inhibitions of 99% and 60% were obtained in vitro and in vivo, respectively. CONCLUSION: The albumin denaturation and carrageenan-induced rat hind paw edema model to assess the anti-inflammatory potential of generated nanoparticles suggests that the silver nanoparticles may act as reducing/inhibiting agents on the release of acute inflammatory mediators. Hence, this work clearly demonstrated that silver nanoparticles mediated-Selaginella myosurus could be considered as a potential source for anti-inflammatory drugs.
format Online
Article
Text
id pubmed-6296690
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-62966902018-12-26 Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract Kedi, Philippe Belle Ebanda Meva, Francois Eya’ane Kotsedi, Lebogang Nguemfo, Edwige Laure Zangueu, Calvin Bogning Ntoumba, Agnes Antoinette Mohamed, Hamza Elsayed Ahmed Dongmo, Alain Bertrand Maaza, Malik Int J Nanomedicine Original Research INTRODUCTION: There is emerging interest in medicinal plants in the biomedical field, due to their multitude of chemicals which show anti-inflammatory, antimicrobial, antiviral, or antitumoral potential. Research on medicinal plants has shown that nanotechnology could offer new solutions in the quality control, delivery aspects, or in sustaining herbal biological activities. This work reports on the preparation and characterization of silver nanoparticle-mediated Selaginella myosurus plant extract. METHODS: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, powder X-ray diffraction, energy dispersive X-ray spectroscopy, high-resolution scanning electron microscopy, high-resolution transmission electron microscopy, and selected area electron diffraction have been used to characterize the prepared silver nanoparticles. The synthetic stability was studied by varying concentrations and pH of reactants. Egg albumin denaturation and carrageenan-induced rat paw edema model were used to ascertain the anti-inflammation. RESULTS: Ultraviolet-visible spectroscopy gave plasmon resonance ranging between 420 and 480 nm while Fourier transform infrared spectroscopy proved nano interface functionalized with organics. The powder X-ray diffraction pattern is in agreement with silver and silver chloride nanoparticles of crystallite size 33.7 nm and 44.2 nm for silver and silver chloride, respectively. Energy dispersive X-ray spectroscopy enables elemental characterization of the particles consisting of silver and silver chloride among main elements. Spherical silver grain of 58.81 nm average size has been depicted with high-resolution scanning electron microscopy and high-resolution transmission electron microscopy. Inhibitions of 99% and 60% were obtained in vitro and in vivo, respectively. CONCLUSION: The albumin denaturation and carrageenan-induced rat hind paw edema model to assess the anti-inflammatory potential of generated nanoparticles suggests that the silver nanoparticles may act as reducing/inhibiting agents on the release of acute inflammatory mediators. Hence, this work clearly demonstrated that silver nanoparticles mediated-Selaginella myosurus could be considered as a potential source for anti-inflammatory drugs. Dove Medical Press 2018-12-12 /pmc/articles/PMC6296690/ /pubmed/30587976 http://dx.doi.org/10.2147/IJN.S174530 Text en © 2018 Belle Ebanda Kedi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Kedi, Philippe Belle Ebanda
Meva, Francois Eya’ane
Kotsedi, Lebogang
Nguemfo, Edwige Laure
Zangueu, Calvin Bogning
Ntoumba, Agnes Antoinette
Mohamed, Hamza Elsayed Ahmed
Dongmo, Alain Bertrand
Maaza, Malik
Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title_full Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title_fullStr Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title_full_unstemmed Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title_short Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated Selaginella myosurus aqueous extract
title_sort eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated selaginella myosurus aqueous extract
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296690/
https://www.ncbi.nlm.nih.gov/pubmed/30587976
http://dx.doi.org/10.2147/IJN.S174530
work_keys_str_mv AT kediphilippebelleebanda ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT mevafrancoiseyaane ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT kotsedilebogang ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT nguemfoedwigelaure ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT zangueucalvinbogning ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT ntoumbaagnesantoinette ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT mohamedhamzaelsayedahmed ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT dongmoalainbertrand ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract
AT maazamalik ecofriendlysynthesischaracterizationinvitroandinvivoantiinflammatoryactivityofsilvernanoparticlemediatedselaginellamyosurusaqueousextract