Cargando…

Detection of Mycobacterium tuberculosis purified ESAT-6 (Rv3875) by magnetic bead-coupled gold nanoparticle-based immuno-PCR assay

PURPOSE: Immuno-PCR (I-PCR), an ultrasensitive method, combines the versatility of ELISA with the exponential amplification capacity of PCR. Coupling of detection antibodies with the reporter DNA is a critical step of I-PCR. Gold nanoparticles (GNPs) and magnetic beads (MBs) are relatively easy to a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Singh, Netrapal, Dahiya, Bhawna, Radhakrishnan, Venkatraman Srinivasan, Prasad, Tulika, Mehta, Promod K
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Dove Medical Press 2018
Materias:	Original Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296691/ https://www.ncbi.nlm.nih.gov/pubmed/30587975 http://dx.doi.org/10.2147/IJN.S181052

Ejemplares similares

The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis
por: Mahmood, Nasir, et al.
Publicado: (2022)

Evaluation of in silico designed inhibitors targeting MelF (Rv1936) against Mycobacterium marinum within macrophages
por: Dharra, Renu, et al.
Publicado: (2019)

Diagnosis of genitourinary tuberculosis: detection of mycobacterial lipoarabinomannan and MPT-64 biomarkers within urine extracellular vesicles by nano-based immuno-PCR assay
por: Kamra, Ekta, et al.
Publicado: (2023)

Transcriptional analysis of ESAT-6 cluster 3 in Mycobacterium smegmatis
por: Maciąg, Anna, et al.
Publicado: (2009)

Development of ESAT-6 Based Immunosensor for the Detection of Mycobacterium tuberculosis
por: Omar, Rishabh Anand, et al.
Publicado: (2021)

ESAT-6 a Major Virulence Factor of Mycobacterium tuberculosis
por: Anes, Elsa, et al.
Publicado: (2023)

Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem
por: Sepulveda, Danna, et al.
Publicado: (2017)

Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of Mycobacterium tuberculosis for serodiagnosis of tuberculosis
por: You, Xiaolong, et al.
Publicado: (2017)

Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins
por: Seghatoleslam, Atefeh, et al.
Publicado: (2016)

Oral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10
por: Uvarova, Elena A., et al.
Publicado: (2013)

Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens
por: Mahmoudi, Shima, et al.
Publicado: (2013)

Human papillomavirus 16 and 18 in squamous cell carcinoma of oral cavity and sexual practices: A pilot study at a Tertiary Care Hospital of North India
por: Parshad, Sanjeev, et al.
Publicado: (2015)

Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients
por: Hemmati, M, et al.
Publicado: (2011)

Prevalence of Mycobacterium tuberculosis infection as measured by the QuantiFERON-TB Gold assay and ESAT-6 free IGRA among adolescents in Mwanza, Tanzania
por: Jeremiah, Kidola, et al.
Publicado: (2021)

Purifying Gold Scrap
por: Evans, E. F.
Publicado: (1906)

Mycobacterium tuberculosis Rv0366c-Rv0367c encodes a non-canonical PezAT-like toxin-antitoxin pair
por: Tandon, Himani, et al.
Publicado: (2019)

Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028–Rv2031 (HspX) operon
por: Mushtaq, Khurram, et al.
Publicado: (2015)

BAT3 Regulates Mycobacterium tuberculosis Protein ESAT-6-Mediated Apoptosis of Macrophages
por: Grover, Ajay, et al.
Publicado: (2012)

An N-acetyltransferase required for ESAT-6 N-terminal acetylation and virulence in Mycobacterium marinum
por: Collars, Owen A., et al.
Publicado: (2023)

ESAT-6 Targeting to DEC205+ Antigen Presenting Cells Induces Specific-T Cell Responses against ESAT-6 and Reduces Pulmonary Infection with Virulent Mycobacterium tuberculosis
por: Silva-Sánchez, Aarón, et al.
Publicado: (2015)

Rv2299c, a novel dendritic cell-activating antigen of Mycobacterium tuberculosis, fused-ESAT-6 subunit vaccine confers improved and durable protection against the hypervirulent strain HN878 in mice
por: Choi, Han-Gyu, et al.
Publicado: (2017)

Investigation of the Rv3065, Rv2942, Rv1258c, Rv1410c, and Rv2459 efflux pump genes expression among multidrug-resistant Mycobacterium tuberculosis clinical isolates
por: Shahi, Fatemeh, et al.
Publicado: (2021)

The Effect of Delivery Systems on the Induction of T Helper 1 Cell Response to an ESAT6-Like Protein Rv3619c and Identification of Its Immunodominant Peptides
por: Safar, Hussain A., et al.
Publicado: (2022)

Inhibition of B6RV2 Leukemia Growth by Immunization with Purified Unique Antigen
por: Uenaka, Akiko, et al.
Publicado: (1992)

Network analysis identifies Rv0324 and Rv0880 as regulators of bedaquiline tolerance in Mycobacterium tuberculosis
por: Peterson, Eliza J.R., et al.
Publicado: (2016)

The structure of Rv3717 reveals a novel amidase from Mycobacterium tuberculosis
por: Kumar, Atul, et al.
Publicado: (2013)

Triclosan-induced genes Rv1686c-Rv1687c and Rv3161c are not involved in triclosan resistance in Mycobacterium tuberculosis
por: Gomez, Andromeda, et al.
Publicado: (2016)

Enhanced serodiagnostic potential of a fusion molecule consisting of Rv1793, Rv2628 and a truncated Rv2608 of Mycobacterium tuberculosis
por: Sulman, Sadaf, et al.
Publicado: (2021)

Introducing the ESAT-6 free IGRA, a companion diagnostic for TB vaccines based on ESAT-6
por: Ruhwald, Morten, et al.
Publicado: (2017)

Fusion of Dendritic Cells Activating Rv2299c Protein Enhances the Protective Immunity of Ag85B-ESAT6 Vaccine Candidate against Tuberculosis
por: Back, Yong Woo, et al.
Publicado: (2020)

Transcription factors Rv0081 and Rv3334 connect the early and the enduring hypoxic response of Mycobacterium tuberculosis
por: Sun, Xian, et al.
Publicado: (2018)

Rv1453 is associated with clofazimine resistance in Mycobacterium tuberculosis
por: Zhang, Lei, et al.
Publicado: (2023)

The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria
por: Gey van Pittius, Nico C, et al.
Publicado: (2001)

Preclinical study and phase I clinical safety evaluation of recombinant Mycobacterium tuberculosis ESAT6 protein
por: Du, Wei-Xin, et al.
Publicado: (2013)

Role of Immuno-Polymerase Chain Reaction (I-PCR) in Resolving Diagnostic Dilemma Between Tuberculoma and Neurocysticercosis: A Case Report
por: Ahlawat, Sonia, et al.
Publicado: (2018)

Humoral Responses to Rv1733c, Rv0081, Rv1735c, and Rv1737c DosR Regulon-Encoded Proteins of Mycobacterium tuberculosis in Individuals with Latent Tuberculosis Infection
por: Kimuda, Simon G., et al.
Publicado: (2017)

A novel recombinant DNA vaccine encoding Mycobacterium tuberculosis ESAT-6 and FL protects against Mycobacterium tuberculosis challenge in mice
por: Jiang, Qingtao, et al.
Publicado: (2013)

Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c Proteins Participate in the Suppression of Macrophage Apoptosis
por: Danelishvili, Lia, et al.
Publicado: (2010)

Antigens Rv0310c and Rv1255c are promising novel biomarkers for the diagnosis of Mycobacterium tuberculosis infection
por: Luo, Liulin, et al.
Publicado: (2017)

MiaA (Rv2727c) mediated tRNA isopentenylation of Mycobacterium tuberculosis H37Rv
por: Soman, Smitha, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_kbg96im0v0karto49jl91h4t47