Cargando…

Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective

OBJECTIVES: Candidiasis is a common human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome-associated opportunistic mycoses. The present study ascertained the species spectrum of Candida strains recovered from different clinical samples from symptomatic HIV-positive individuals and de...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaur, Ravinder, Mehra, Bhanu, Dhakad, Megh Singh, Goyal, Ritu, Bhalla, Preena, Dewan, Richa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298152/
https://www.ncbi.nlm.nih.gov/pubmed/30623182
http://dx.doi.org/10.4103/ijstd.IJSTD_89_17
_version_ 1783381262070710272
author Kaur, Ravinder
Mehra, Bhanu
Dhakad, Megh Singh
Goyal, Ritu
Bhalla, Preena
Dewan, Richa
author_facet Kaur, Ravinder
Mehra, Bhanu
Dhakad, Megh Singh
Goyal, Ritu
Bhalla, Preena
Dewan, Richa
author_sort Kaur, Ravinder
collection PubMed
description OBJECTIVES: Candidiasis is a common human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome-associated opportunistic mycoses. The present study ascertained the species spectrum of Candida strains recovered from different clinical samples from symptomatic HIV-positive individuals and determined the antifungal susceptibility profile of the isolates. MATERIALS AND METHODS: A variety of specimens were collected from 234 symptomatic HIV seropositive individuals depending on their clinical manifestations and subjected to direct microscopic examination. Blood samples were inoculated in biphasic blood culture medium and all other specimens on Sabouraud dextrose agar with chloramphenicol and incubated at 35°C–37°C. Species identification of the recovered Candida isolates was attempted on the basis of germ tube production, micromorphology on corn meal agar, color and morphology on HiCrome Candida Differential agar, and carbohydrate fermentation and assimilation tests. Susceptibility testing of the isolates was performed employing the VITEK 2 system. RESULTS: A total of 167 Candida isolates were obtained; Candida albicans (136), Candida tropicalis (13), Candida krusei (8), Candida parapsilosis (5), Candida glabrata (4), and Candida kefyr (1). Fluconazole resistance was more frequent among nonalbicans species, and significantly higher 5-fluorocytosine resistance compared to C. albicans was also observed. Eight Candida strains (six C. krusei, one C. kefyr, and one C. albicans) were multidrug resistant. CONCLUSION: Although C. albicans continues to be the leading etiological agent of candidiasis, the incidence of nonalbicans species among HIV-positive Indian individuals is rising. Antifungal resistance was higher among nonalbicans Candida species. Another issue of therapeutic concern is the possible emergence of multidrug-resistant Candida strains among these patients.
format Online
Article
Text
id pubmed-6298152
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-62981522019-01-08 Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective Kaur, Ravinder Mehra, Bhanu Dhakad, Megh Singh Goyal, Ritu Bhalla, Preena Dewan, Richa Indian J Sex Transm Dis AIDS Original Article OBJECTIVES: Candidiasis is a common human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome-associated opportunistic mycoses. The present study ascertained the species spectrum of Candida strains recovered from different clinical samples from symptomatic HIV-positive individuals and determined the antifungal susceptibility profile of the isolates. MATERIALS AND METHODS: A variety of specimens were collected from 234 symptomatic HIV seropositive individuals depending on their clinical manifestations and subjected to direct microscopic examination. Blood samples were inoculated in biphasic blood culture medium and all other specimens on Sabouraud dextrose agar with chloramphenicol and incubated at 35°C–37°C. Species identification of the recovered Candida isolates was attempted on the basis of germ tube production, micromorphology on corn meal agar, color and morphology on HiCrome Candida Differential agar, and carbohydrate fermentation and assimilation tests. Susceptibility testing of the isolates was performed employing the VITEK 2 system. RESULTS: A total of 167 Candida isolates were obtained; Candida albicans (136), Candida tropicalis (13), Candida krusei (8), Candida parapsilosis (5), Candida glabrata (4), and Candida kefyr (1). Fluconazole resistance was more frequent among nonalbicans species, and significantly higher 5-fluorocytosine resistance compared to C. albicans was also observed. Eight Candida strains (six C. krusei, one C. kefyr, and one C. albicans) were multidrug resistant. CONCLUSION: Although C. albicans continues to be the leading etiological agent of candidiasis, the incidence of nonalbicans species among HIV-positive Indian individuals is rising. Antifungal resistance was higher among nonalbicans Candida species. Another issue of therapeutic concern is the possible emergence of multidrug-resistant Candida strains among these patients. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6298152/ /pubmed/30623182 http://dx.doi.org/10.4103/ijstd.IJSTD_89_17 Text en Copyright: © 2018 Indian Journal of Sexually Transmitted Diseases and AIDS http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kaur, Ravinder
Mehra, Bhanu
Dhakad, Megh Singh
Goyal, Ritu
Bhalla, Preena
Dewan, Richa
Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title_full Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title_fullStr Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title_full_unstemmed Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title_short Clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: An Indian perspective
title_sort clinico-mycological analysis and antifungal resistance pattern in human immunodeficiency virus-associated candidiasis: an indian perspective
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298152/
https://www.ncbi.nlm.nih.gov/pubmed/30623182
http://dx.doi.org/10.4103/ijstd.IJSTD_89_17
work_keys_str_mv AT kaurravinder clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective
AT mehrabhanu clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective
AT dhakadmeghsingh clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective
AT goyalritu clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective
AT bhallapreena clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective
AT dewanricha clinicomycologicalanalysisandantifungalresistancepatterninhumanimmunodeficiencyvirusassociatedcandidiasisanindianperspective