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The role of RNA structure in translational regulation by L7Ae protein in archaea

A recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5′-leader of the mRNA of its structural gene to regulate translation. To function as a regulator, the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem–loop on bin...

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Detalles Bibliográficos
Autores principales: Huang, Lin, Ashraf, Saira, Lilley, David M.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298567/
https://www.ncbi.nlm.nih.gov/pubmed/30327333
http://dx.doi.org/10.1261/rna.068510.118
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author Huang, Lin
Ashraf, Saira
Lilley, David M.J.
author_facet Huang, Lin
Ashraf, Saira
Lilley, David M.J.
author_sort Huang, Lin
collection PubMed
description A recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5′-leader of the mRNA of its structural gene to regulate translation. To function as a regulator, the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem–loop on binding L7Ae. Sequence analysis of UTR sequences indicates that their k-turn elements will be unable to fold in the absence of L7Ae, and we have demonstrated this experimentally in solution using FRET for the Archaeoglobus fulgidus sequence. We have solved the X-ray crystal structure of the complex of the A. fulgidus RNA bound to its cognate L7Ae protein. The RNA adopts a standard k-turn conformation that is specifically recognized by the L7Ae protein, so stabilizing the stem–loop. In-line probing of the natural-sequence UTR shows that the RNA is unstructured in the absence of L7Ae binding, but folds on binding the protein such that the ribosome binding site is occluded. Thus, L7Ae regulates its own translation by switching the conformation of the RNA to alter accessibility.
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spelling pubmed-62985672019-01-01 The role of RNA structure in translational regulation by L7Ae protein in archaea Huang, Lin Ashraf, Saira Lilley, David M.J. RNA Article A recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5′-leader of the mRNA of its structural gene to regulate translation. To function as a regulator, the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem–loop on binding L7Ae. Sequence analysis of UTR sequences indicates that their k-turn elements will be unable to fold in the absence of L7Ae, and we have demonstrated this experimentally in solution using FRET for the Archaeoglobus fulgidus sequence. We have solved the X-ray crystal structure of the complex of the A. fulgidus RNA bound to its cognate L7Ae protein. The RNA adopts a standard k-turn conformation that is specifically recognized by the L7Ae protein, so stabilizing the stem–loop. In-line probing of the natural-sequence UTR shows that the RNA is unstructured in the absence of L7Ae binding, but folds on binding the protein such that the ribosome binding site is occluded. Thus, L7Ae regulates its own translation by switching the conformation of the RNA to alter accessibility. Cold Spring Harbor Laboratory Press 2019-01 /pmc/articles/PMC6298567/ /pubmed/30327333 http://dx.doi.org/10.1261/rna.068510.118 Text en © 2019 Huang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Huang, Lin
Ashraf, Saira
Lilley, David M.J.
The role of RNA structure in translational regulation by L7Ae protein in archaea
title The role of RNA structure in translational regulation by L7Ae protein in archaea
title_full The role of RNA structure in translational regulation by L7Ae protein in archaea
title_fullStr The role of RNA structure in translational regulation by L7Ae protein in archaea
title_full_unstemmed The role of RNA structure in translational regulation by L7Ae protein in archaea
title_short The role of RNA structure in translational regulation by L7Ae protein in archaea
title_sort role of rna structure in translational regulation by l7ae protein in archaea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298567/
https://www.ncbi.nlm.nih.gov/pubmed/30327333
http://dx.doi.org/10.1261/rna.068510.118
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